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URL: http://www.addgene.org/128255
Proper Citation: RRID:Addgene_128255
Insert Name: hTetR::P2A::EGFP::T2A::PuroR
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:31235692
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:7547; Vector Backbone:pDN-D2irTN2aG5kwh; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Comments: The plasmid encodes a synthetic gene circuit controlling the PuroR gene using negative feedback through a humanized tetracycline repressor (hTetR) self-repressing a CMV-derived promoter with 2 tetO2 sites (CMV-D2ir). The circuit is also called mNF-PuroR.
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No rating or validation information has been found for pKF-D2irTNP2AG-T2APuroR-5kwh.
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Source: Addgene