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URL: http://www.addgene.org/128254
Proper Citation: RRID:Addgene_128254
Insert Name: rtTA-Advanced::P2A::EGFP
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:31235692
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5070; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Comments: The plasmid encodes a mammalian synthetic gene circuit that contains the regulator reverse tetracycline Trans Activator (rtTA) and the reporter EGFP, which is controlled by a tight TRE promoter that rtTA binds to activate its own expression in a positive feedback loop. The backbone consists of the Flp-In expression vector (pcDNA5/FRT). The synthetic gene circuit is also called mPF-GFP.
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Source: Addgene
