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URL: http://www.addgene.org/127520
Proper Citation: RRID:Addgene_127520
Insert Name: CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
Organism: Arabidopsis thaliana
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32444777
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Comments: The CaMV 35S promoter in a reverse complement sequence orientation came from iGEM registry (BBa_K1547006 ) and the attB/P integrases 2, 4 and 5 attatchment sites flanking this sequence came from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014). This cassete was previously synthesized in pBluescript II SK(-) and after cloned using HindIII and BamHI replacing the forward CaMV 35S promoter sequence from a 35S-GFP plasmid construction of Chiu, W. et al. Curr. Biol. 6, 325-330 (1996).
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No rating or validation information has been found for INCTbiosyn-p35S(rc)2_4_5-GFP.
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Source: Addgene
