Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
URL: http://www.addgene.org/118155
Proper Citation: RRID:Addgene_118155
Insert Name: KRAB-dCas9-P2A-BlastR
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:31253982
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #52961); Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: We have also generated a version of this backbone with the core EF1a promoter driving the expression of KRAB-dCas9. We advise users to test both backbones in their cell type of interest. In our experience this backbone yields better knockdown in liver and pancreatic beta cells. Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint. We have also made available plasmids to be used as negative controls, which contain non-human genome target sequences (BFP or EGFP). Please see Addgene #118161, #118162, #118163 and #118164. There is a third BsmBI site in the promoter region, which does not have an impact on the GoldenGate cloning. We recommend using the provided cloning protocol. Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.
Expand AllWe found {{ ctrl2.mentions.all_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR.
No alerts have been found for Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR.
Source: Addgene
