URL: https://cm.jefferson.edu/Off-Spotter//
Proper Citation: Off-Spotter (RRID:SCR_015739)
Description: Web application that identifies genomic instances for a given combination of gRNA(s), PAM, number of mismatches, and seed. This tool is limited to a single 1,000 nucleotides sequence or fewer than twenty CR-separated 20-mers.
Synonyms: Off-Spotter: tool for CRISPR/Cas design, Off-spotter sgRNA algorithm
Resource Type: algorithm resource, software resource, web application
Defining Citation: PMID:25630343
Keywords: CRISPR, Cas, sgrna, prokaryotic immune system, genetic engineering, bio.tools
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