URL: http://last.cbrc.jp/
Proper Citation: LAST (RRID:SCR_006119)
Description: THIS RESOURCE IS NO LONGER IN SERVICE. Documented on February 28,2023. Software tool for aligning sequences, similar to BLAST 2 sequences that colour-codes the alignments by reliability. Another useful feature of LAST is that it can compare huge (vertebrate-genome-sized) datasets. Unfortunately, this only applies to the downloadable version of LAST, not the web service. The web service can just about handle bacterial genomes, but it will take a few minutes and the output will be large. LAST can: * Handle big sequence data, e.g: ** Compare two vertebrate genomes ** Align billions of DNA reads to a genome * Indicate the reliability of each aligned column. * Use sequence quality data properly. * Compare DNA to proteins, with frameshifts. * Compare PSSMs to sequences * Calculate the likelihood of chance similarities between random sequences. LAST cannot (yet): * Do spliced alignment.
Abbreviations: LAST
Resource Type: software resource, software application, analysis service resource, service resource, production service resource, data processing software, data analysis service
Defining Citation: PMID:21209072, PMID:20144198, PMID:20110255, DOI:10.1093/nar/gkq010
Keywords: sequence alignment, align, vertebrate, genome, sequence, alignment, bio.tools
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National Institute of Advanced Industrial Science and Technology |
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