Burdock miR8175 in diet improves insulin resistance induced by obesity in mice through food absorption.
The incidence of type 2 diabetes mellitus (T2DM) induced by obesity is rapidly increasing. Although there are many synthetic drugs for treating T2DM, they have various side effects. Here, we report that miR8175, a plant miRNA from burdock root, has effective antidiabetic activity. After administration of burdock decoction or synthetic miR8175 by gavage, both burdock decoction and miR8175 can significantly improve the impaired glucose metabolism of diabetic mice induced by a high-fat diet (HFD). Our results demonstrate that burdock decoction and miR8175 enhance the insulin sensitivity of the hepatic insulin signaling pathway by targeting Ptprf and Ptp1b, which may be the reason for the improvement in metabolism. This study provides a theoretical basis for the main active component and molecular mechanism of burdock to improve insulin resistance. And the study also suggests that plant miRNA may be an indispensable nutrient for maintaining human health.
Pubmed ID: 38660399 RIS Download
Research resources used in this publication
Additional research tools detected in this publication
Antibodies used in this publication
- Rabbit Anti-IRS1, phospho (Tyr612) Polyclonal Antibody, Unconjugated (RRID:AB_1140753)
- donkey anti-mouse IgG-HRP (RRID:AB_641170)
- donkey anti-rabbit IgG-HRP (RRID:AB_631745)
- actin (RRID:AB_2313904)
- PTP1B Antibody (RRID:AB_10695100)
- PTPRF/LAR Antibody (RRID:AB_2798778)
- Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (RRID:AB_2315049)
- IRS1 (phospho Y896) antibody [EP260Y] (RRID:AB_881460)
- IGF-I Receptor (D23H3) XP Rabbit mAb (RRID:AB_10950969)
- Phospho-IGF-I Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151) (19H7) Rabbit mAb (RRID:AB_331253)
- Akt Antibody (RRID:AB_329827)
- SOCS3 (D6E1T) Rabbit mAb (RRID:AB_2799408)
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ATCC (tool)
RRID:SCR_001672
Global nonprofit biological resource center (BRC) and research organization that provides biological products, technical services and educational programs to private industry, government and academic organizations. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement and application of scientific knowledge. The primary purpose of ATCC is to use its resources and experience as a BRC to become the world leader in standard biological reference materials management, intellectual property resource management and translational research as applied to biomaterial development, standardization and certification. ATCC characterizes cell lines, bacteria, viruses, fungi and protozoa, as well as develops and evaluates assays and techniques for validating research resources and preserving and distributing biological materials to the public and private sector research communities.
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RRID:SCR_002750
A free reference manager and academic social network to organize your research, collaborate with others online, and discover the latest research. Automatically generate bibliographies, Collaborate easily with other researchers online, Easily import papers from other research software, Find relevant papers based on what you're reading, Access your papers from anywhere online, Read papers on the go with the iPhone app. The software, Mendeley Desktop, offers: * Automatic extraction of document details * Efficient management of your papers * Sharing and synchronization of your library (or parts of it) * Additional features: A plug-in for citing your articles in Microsoft Word, OCR (image-to-text conversion, so you can full-text search all your scanned PDFs), etc The website, Mendeley Web, complements Mendeley Desktop by offering these features: * An online back up of your library * Statistics of all things interesting * A research network that allows you to keep track of your colleagues' publications, conference participations, awards etc * A recommendation engine for papers that might interest you
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RRID:IMSR_JAX:000664
Mus musculus with name C57BL/6J from IMSR.
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RRID:AB_1140753
This polyclonal targets IRS1 (phospho Y612)
View all literature mentionsdonkey anti-mouse IgG-HRP (antibody)
RRID:AB_641170
This polyclonal targets donkey anti-mouse IgG-HRP
View all literature mentionsdonkey anti-rabbit IgG-HRP (antibody)
RRID:AB_631745
This polyclonal targets Rabbit IgG
View all literature mentionsPTP1B Antibody (antibody)
RRID:AB_10695100
This polyclonal targets PTP1B
View all literature mentionsPTPRF/LAR Antibody (antibody)
RRID:AB_2798778
This unknown targets PTPRF
View all literature mentionsPhospho-Akt (Ser473) (D9E) XP® Rabbit mAb (antibody)
RRID:AB_2315049
This monoclonal targets Phospho-Akt (Ser473)
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RRID:AB_881460
This monoclonal targets IRS1 (phospho Y896) antibody [EP260Y]
View all literature mentionsIGF-I Receptor (D23H3) XP Rabbit mAb (antibody)
RRID:AB_10950969
This monoclonal targets IGF-I Receptor (D23H3) XP Rabbit mAb
View all literature mentionsPhospho-IGF-I Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151) (19H7) Rabbit mAb (antibody)
RRID:AB_331253
This monoclonal targets Phospho-IGF-I Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151) (19H7) Rabbit mAb
View all literature mentionsSOCS3 (D6E1T) Rabbit mAb (antibody)
RRID:AB_2799408
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RRID:SCR_003070
Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
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RRID:SCR_002798
Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
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