Oncogenic transformation is associated with profound changes in cellular metabolism, but whether tracking these can improve disease stratification or influence therapy decision-making is largely unknown. Using the iKnife to sample the aerosol of cauterized specimens, we demonstrate a new mode of real-time diagnosis, coupling metabolic phenotype to mutant PIK3CA genotype. Oncogenic PIK3CA results in an increase in arachidonic acid and a concomitant overproduction of eicosanoids, acting to promote cell proliferation beyond a cell-autonomous manner. Mechanistically, mutant PIK3CA drives a multimodal signaling network involving mTORC2-PKCζ-mediated activation of the calcium-dependent phospholipase A2 (cPLA2). Notably, inhibiting cPLA2 synergizes with fatty acid-free diet to restore immunogenicity and selectively reduce mutant PIK3CA-induced tumorigenicity. Besides highlighting the potential for metabolic phenotyping in stratified medicine, this study reveals an important role for activated PI3K signaling in regulating arachidonic acid metabolism, uncovering a targetable metabolic vulnerability that largely depends on dietary fat restriction. VIDEO ABSTRACT.
Pubmed ID: 32559461 RIS Download
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Multi paradigm numerical computing environment and fourth generation programming language developed by MathWorks. Allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. Used to explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.
View all literature mentionsGlobal nonprofit biological resource center (BRC) and research organization that provides biological products, technical services and educational programs to private industry, government and academic organizations. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement and application of scientific knowledge. The primary purpose of ATCC is to use its resources and experience as a BRC to become the world leader in standard biological reference materials management, intellectual property resource management and translational research as applied to biomaterial development, standardization and certification. ATCC characterizes cell lines, bacteria, viruses, fungi and protozoa, as well as develops and evaluates assays and techniques for validating research resources and preserving and distributing biological materials to the public and private sector research communities.
View all literature mentionsBioinformatics resource system including web server and web service for functional annotation and enrichment analyses of gene lists. Consists of comprehensive knowledgebase and set of functional analysis tools. Includes gene centered database integrating heterogeneous gene annotation resources to facilitate high throughput gene functional analysis.
View all literature mentionsSoftware environment and programming language for statistical computing and graphics. R is integrated suite of software facilities for data manipulation, calculation and graphical display. Can be extended via packages. Some packages are supplied with the R distribution and more are available through CRAN family.It compiles and runs on wide variety of UNIX platforms, Windows and MacOS.
View all literature mentionsNon-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
View all literature mentionsA free reference manager and academic social network to organize your research, collaborate with others online, and discover the latest research. Automatically generate bibliographies, Collaborate easily with other researchers online, Easily import papers from other research software, Find relevant papers based on what you're reading, Access your papers from anywhere online, Read papers on the go with the iPhone app. The software, Mendeley Desktop, offers: * Automatic extraction of document details * Efficient management of your papers * Sharing and synchronization of your library (or parts of it) * Additional features: A plug-in for citing your articles in Microsoft Word, OCR (image-to-text conversion, so you can full-text search all your scanned PDFs), etc The website, Mendeley Web, complements Mendeley Desktop by offering these features: * An online back up of your library * Statistics of all things interesting * A research network that allows you to keep track of your colleagues' publications, conference participations, awards etc * A recommendation engine for papers that might interest you
View all literature mentionsPrivately held company that develops and produces antibodies, ELISA kits, ChIP kits, proteomic kits, and other related reagents used to study cell signaling pathways that impact human health.
View all literature mentionsA multi-institutional effort to identify and quantitate, using a systems biology approach and sophisticated mass spectrometers, all of the major - and many minor - lipid species in mammalian cells, as well as to quantitate the changes in these species in response to perturbation. The goal of their research is to better understand lipid metabolism and the active role lipids play in diabetes, stroke, cancer, arthritis, Alzheimer's and other lipid-based diseases in order to facilitate development of more effective treatments. Resources available include: LIPID MAPS publications, detailed biochemical pathways, improved protocols for lipid separation and quantification, analytical tools for determining lipid quantitation, structure drawing tools for automatically drawing lipid molecular structures in stereochemical detail, and experimental data. The LIPID MAPS organization includes six lipidomics core laboratories, each specialized in extracting, identifying, and quantifying one of the major categories of mammalian lipids: fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterol lipids, and prenol lipids. Other core laboratories and bridge projects include bioinformatics, mass spectrometric imaging, lipid synthesis, oxidized lipids, and macrophage biology and genomics.
View all literature mentionsA commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.
View all literature mentionsSoftware that enables rapid tool creation by providing a robust, pluggable development framework that simplifies and unifies data file access, and performs standard proteomics and LCMS dataset computations.
View all literature mentionsCell line MCF-10A is a Spontaneously immortalized cell line with a species of origin Homo sapiens
View all literature mentionslaboratory mouse with name BALB/cAnNCrl from MGI.
View all literature mentionsThis unknown targets PLC gamma 1
View all literature mentionsThis recombinant monoclonal targets GAPDH
View all literature mentionsThis monoclonal targets HA-Tag
View all literature mentionsThis unknown targets normal rabbit IgG
View all literature mentionsThis monoclonal targets p-RKIP (80.Ser 153)
View all literature mentionsThis polyclonal targets Pla2g1b
View all literature mentionsThis polyclonal targets p38 MAPK
View all literature mentionsThis monoclonal targets Human PRKCB
View all literature mentionsThis monoclonal targets 4E-BP1 (53H11) Rabbit mAb
View all literature mentionsThis polyclonal targets Pla2g1b
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets p38 MAPK, phospho (Thr180 / Tyr182)
View all literature mentionsThis polyclonal targets Erk1/2
View all literature mentionsThis polyclonal targets p44/42 MAPK (Erk1/2)
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis polyclonal targets Mouse NKp46 / NCR1
View all literature mentionsThis monoclonal targets Akt1s1
View all literature mentionsThis polyclonal targets PKC epsilon (phospho S729) antibody
View all literature mentionsThis monoclonal targets mTOR
View all literature mentionsThis polyclonal targets Phospho-MEK1/2 (Ser217/221)
View all literature mentionsThis polyclonal targets Rictor
View all literature mentionsThis polyclonal targets MEK1/2
View all literature mentionsThis monoclonal targets Raptor (24C12) Rabbit mAb
View all literature mentionsThis monoclonal targets c-Raf, phospho (Ser338)
View all literature mentionsThis polyclonal targets PKC epsilon
View all literature mentionsThis monoclonal targets PKC zeta (phospho T560) antibody [EP2037AY]
View all literature mentionsThis polyclonal targets c-Raf
View all literature mentionsThis monoclonal targets PRKD1
View all literature mentionsThis monoclonal targets Phospho-NDRG1 (Thr346) (D98G11) XP Rabbit mAb
View all literature mentionsThis polyclonal targets PKC zeta
View all literature mentionsThis polyclonal targets NDRG1
View all literature mentionsThis polyclonal targets Lipocalin 2 antibody
View all literature mentionsThis polyclonal targets RKIP
View all literature mentionsThis polyclonal targets Phospho-S6 Ribosomal Protein (Ser235/236)
View all literature mentionsThis monoclonal targets Rb (4H1) Mouse mAb
View all literature mentionsThis polyclonal targets Prkcd
View all literature mentionsThis unknown targets Goat Mouse IgG (H L)-HRP Conjugate
View all literature mentionsThis monoclonal targets S6 Ribosomal Protein (54D2) Mouse mAb
View all literature mentionsThis unknown targets PKD / PKC mu, phospho (Ser744 / Ser748)
View all literature mentionsThis unknown targets PKC delta
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Phospho-PKC (pan) (βII Ser660)
View all literature mentionsThis monoclonal targets Phospho-PKC zeta (Thr410)
View all literature mentionsThis polyclonal targets Phospholipase A2 (iPLA2) antibody produced in rabbit
View all literature mentionsThis monoclonal targets Lamin B1
View all literature mentionsThis polyclonal targets Phospho-4E-BP1 (Ser65)
View all literature mentionsThis polyclonal targets Phospho-p70 S6 Kinase (Thr389)
View all literature mentionsThis polyclonal targets Phospho-Stat3 (Ser727)
View all literature mentionsThis unknown targets PRAS40
View all literature mentionsThis polyclonal targets p70 S6 Kinase
View all literature mentionsThis monoclonal targets Phospho-Akt (Ser473)
View all literature mentionsThis recombinant monoclonal targets Stat3
View all literature mentionsThis polyclonal targets β-actin
View all literature mentionsThis monoclonal targets Phospho-I B (Ser32)
View all literature mentionsThis unknown targets IkappaB-alpha
View all literature mentionsThis polyclonal targets Phospho-Threonine/Tyrosine
View all literature mentionsThis polyclonal targets Phospho-PLCgamma1 (Tyr783)
View all literature mentionsCell line HCC70 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line CAMA-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line CAL-51 is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsCell line HCC38 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line BT-549 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line BT-474 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC202 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line BT-20 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line AU565 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line VP229 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1954 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1937 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line UACC-812 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1569 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line T-47D is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1500 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SK-BR-3 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line S68 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-361 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1428 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MFM-223 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-468 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1395 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-453 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-436 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCC1143 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line BT-483 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SUM229PE is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SUM225CWN is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-231 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-157 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SUM149PT is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SUM159PT is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-134-VI is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SUM44PE is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MCF-7 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line ZR-75-30 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line KPL-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line JIMT-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line ZR-75-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line Hs 578T is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line EFM-19 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SUM52PE is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SUM44PE is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentions