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Draper antibody - Logan, M.; Oregon Health and Science University

RRID:AB_2618106

Antibody ID

AB_2618106

Target Antigen

Draper drosophila

Proper Citation

(DSHB Cat# Draper 8A1, RRID:AB_2618106)

Clonality

monoclonal antibody

Comments

Application(s): Immunofluorescence,Western Blot; Date Deposited: 12/13/2012

Host Organism

mouse

Vendor

DSHB Go To Vendor

Cat Num

Draper 8A1

Publications that use this research resource

A novel Drosophila injury model reveals severed axons are cleared through a Draper/MMP-1 signaling cascade.

  • Purice MD
  • Elife
  • 2017 Aug 21

Literature context:


Abstract:

Neural injury triggers swift responses from glia, including glial migration and phagocytic clearance of damaged neurons. The transcriptional programs governing these complex innate glial immune responses are still unclear. Here, we describe a novel injury assay in adult Drosophila that elicits widespread glial responses in the ventral nerve cord (VNC). We profiled injury-induced changes in VNC gene expression by RNA sequencing (RNA-seq) and found that responsive genes fall into diverse signaling classes. One factor, matrix metalloproteinase-1 (MMP-1), is induced in Drosophila ensheathing glia responding to severed axons. Interestingly, glial induction of MMP-1 requires the highly conserved engulfment receptor Draper, as well as AP-1 and STAT92E. In MMP-1 depleted flies, glia do not properly infiltrate neuropil regions after axotomy and, as a consequence, fail to clear degenerating axonal debris. This work identifies Draper-dependent activation of MMP-1 as a novel cascade required for proper glial clearance of severed axons.