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Modulation of intestinal health and metabolism by dietary Portulaca oleracea L. extract enhances growth performance, immune function, and meat quality in Wenchang chickens.

Yu Zhang | Yaxian Yang | Yinming Li | Yanling Sun | Xiaoyun Han | Hailong Liu | Xinghua Zhao | Yan Zhang | Xin He
Poultry science | 2026

Portulaca oleracea L. extract (POE) shows potential for enhancing poultry production, but its effects on Wenchang chickens are unclear. A total of 90 one-day-old female Wenchang chickens were randomly allocated into three treatment groups: a control group (CON) receiving a basal diet, and two experimental groups (POL and POH) receiving the basal diet supplemented with 0.2 % and 0.4 % POE, respectively. Compared to the CON group, the POL group exhibited significantly higher average daily gain (ADG) and lower feed conversion ratio (FCR) (P < 0.05). POL also significantly enhanced immune function: increased serum immunoglobulins (IgG, IgA, IgM), cytokines (IL-1, IL-4, IFN-γ), secretory IgA (sIgA), and spleen index (P < 0.05). Meat quality improved in POL, with reduced drip loss, cooking loss, and shear force, alongside increased flavor-enhancing amino acids and inosine monophosphate (IMP) (P < 0.05). Jejunal morphology (villus height, VH/CD ratio) and tight junction protein (Claudin-1, Occludin, ZO-1) expression were upregulated in POL (P < 0.05). Gut microbiota analysis revealed POL increased Bacteroides and Faecalibacterium abundance while lowering the Firmicutes / Bacteroidota ratio (P < 0.05). Metabolomics indicated enrichment in tryptophan, arginine, and proline metabolism pathways in POL. Dietary 0.2 % POE improved growth, immunity, and meat quality in Wenchang chickens, potentially mediated by enhanced intestinal health and modulation of tryptophan, arginine, and proline metabolism.

Pubmed ID: 41265143

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