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Welcome to the PRECISE-TBI preclinical TBI protocol resource page!
This resource contains curated lists of preclinical TBI model protocols to encourage reproducibility in preclinical TBI research. The curated preclinical TBI protocols are dynamic centralized lists of preclinical protocols that the PRECISE-TBI team has reviewed. These lists consist of protocols assembled from (1) peer-reviewed literature and (2) created and standardized within. Please select text to expand the list.
We are inviting investigators to submit protocols to the PRECISE-TBI !!!
Please provide your contact information here to start the process.
FAQ - protocols.io
- What is protocols.io?
Protocols.io is an open-access repository for sharing and collaborating on experimental methods and protocols. Currently, there are over 450 journals linked to protocols.io where authors can deposit their full protocol and obtain a Digital Object Identifier, DOI, that can be cited in scholarly articles. - Why is PRECISE-TBI using protocols.io?
To enhance Findable, Accessible, Interoperable, Reproducible (FAIR) data standards, protocols.io provides a unique DOI to facilitate citations in the methods sections of research manuscripts the ability for protocols to copy, version, or fork be forked or cloned in protocol.io. - Version - A protocol version allows “anyone to create a new version of a protocol. However, only the owner of the original protocol can publish new versions. All versions of a protocol will be linked with each other. If another user makes a new version of your protocol, they must request that the version being merged. The protocol owner will see a request for the merge and can decide if they want to accept the new version and whether to publish it or not.”
- Fork - “A protocol fork is an editable version of the protocol that you can modify to your needs. The fork will include a note under the protocol’s title that links back to the protocol it was forked from; this ensures that the original protocol author can track reuse and receive credit for it.”
- Copy - A protocol copy is a functionality that only exists for the contact/co-admins on the protocol. You can duplicate your protocol and modify it as needed.”
Versions and forks can be assigned a unique DOI to facilitate citations in the methods sections of research manuscripts.
Please see the protocols.io website for more information on this functionality.
All tutorial videos: https://www.protocols.io/tutorials
Webinars: https://www.protocols.io/webinars
Additional protocols.io FAQ: https://www.protocols.io/help/faq
PRECISE-TBI protocols on Protocols.io
The protocols below are created in the PRECISE-TBI workspace in Protocols.io.
Controlled Cortical Impact Model Protocol
Fluid Percussion Model Protocol
- Fluid Percussion Model in rat - Lyeth Lab - dx.doi.org/10.17504/protocols.io.rm7vzb2r5vx1/v1
Blast Injury Model Protocol
- Open-field blast (OFB) model in mice- Available at dx.doi.org/10.17504/protocols.io.yxmvm2kwog3p/v1
- Advanced Blast Stimulator in rat - VandeVord Lab - Coming soon
Weight-Drop Model Protocol
- Closed Head Weight Drop Mmodel in mice - Whalen Lab - dx.doi.org/10.17504/protocols.io.j8nlkw2pdl5r/v1
Published peer-reviewed protocols
The protocols below were assembled from previously published literature to unveil these protocols in a centralized place.
Controlled Cortical Impact Model Protocol
- Alluri H, Shaji CA, Davis ML, Tharakan B. A Mouse Controlled Cortical Impact Model of Traumatic Brain Injury for Studying Blood-Brain Barrier Dysfunctions. Methods Mol Biol. 2018;1717:37-52. doi: 10.1007/978-1-4939-7526-6_4. PMID: 29468582.
- Baker EW, Kinder HA, Hutcheson JM, Duberstein KJJ, Platt SR, Howerth EW, West FD. Controlled Cortical Impact Severity Results in Graded Cellular, Tissue, and Functional Responses in a Piglet Traumatic Brain Injury Model. J Neurotrauma. 2019 Jan 1;36(1):61-73. doi: 10.1089/neu.2017.5551. Epub 2018 Aug 21. PMID: 29916303.
- Brody DL, Mac Donald C, Kessens CC, Yuede C, Parsadanian M, Spinner M, Kim E, Schwetye KE, Holtzman DM, Bayly PV. Electromagnetic controlled cortical impact device for precise, graded experimental traumatic brain injury. J Neurotrauma. 2007 Apr;24(4):657-73. doi: 10.1089/neu.2006.0011. PMID: 17439349; PMCID: PMC2435168.
- Campolo M, Esposito E, Cuzzocrea S. A Controlled Cortical Impact Preclinical Model of Traumatic Brain Injury. Methods Mol Biol. 2018;1727:385-391. doi: 10.1007/978-1-4939-7571-6_30. PMID: 29222798.
- Chen Y, Mao H, Yang KH, Abel T, Meaney DF. A modified controlled cortical impact technique to model mild traumatic brain injury mechanics in mice. Front Neurol. 2014 Jun 18;5:100. doi: 10.3389/fneur.2014.00100. PMID: 24994996; PMCID: PMC4061598.
- Dean DD, Frank JA, Turtzo LC. Controlled Cortical Impact in the Rat. Curr Protoc Neurosci. 2017 Oct 23;81:9.62.1-9.62.12. doi: 10.1002/cpns.37. PMID: 29058772; PMCID: PMC5701654.
- Fournier ML, Clément T, Aussudre J, Plesnila N, Obenaus A, Badaut J. Contusion Rodent Model of Traumatic Brain Injury: Controlled Cortical Impact. Methods Mol Biol. 2021;2193:49-65. doi: 10.1007/978-1-0716-0845-6_6. PMID: 32808258.
- Jiang J, Dai C, Niu X, Sun H, Cheng S, Zhang Z, Zhu X, Wang Y, Zhang T, Duan F, Chen X, Zhang S. Establishment of a precise novel brain trauma model in a large animal based on injury of the cerebral motor cortex. J Neurosci Methods. 2018 Sep 1;307:95-105. doi: 10.1016/j.jneumeth.2018.06.025. Epub 2018 Jun 28. PMID: 29960029.
- Kim Y, Fu AH, Tucker LB, Liu J, McCabe JT. Characterization of controlled cortical impact devices by high-speed image analysis. J Neurosci Res. 2018 Apr;96(4):501-511. doi: 10.1002/jnr.24099. Epub 2017 Jul 4. PMID: 28675644.
- Lee HF, Chen CH, Chang CF. A Preclinical Controlled Cortical Impact Model for Traumatic Hemorrhage Contusion and Neuroinflammation. J Vis Exp. 2020 Jun 10;(160). doi: 10.3791/61393. PMID: 32597878.
- Manley GT, Rosenthal G, Lam M, Morabito D, Yan D, Derugin N, Bollen A, Knudson MM, Panter SS. Controlled cortical impact in swine: pathophysiology and biomechanics. J Neurotrauma. 2006 Feb;23(2):128-39. doi: 10.1089/neu.2006.23.128. PMID: 16503797.
- Marklund N. Rodent Models of Traumatic Brain Injury: Methods and Challenges. Methods Mol Biol. 2016;1462:29-46. doi: 10.1007/978-1-4939-3816-2_3. PMID: 27604711.Note; methods for CCI LFP, and weight drip models.
- Osier N, Dixon CE. The Controlled Cortical Impact Model of Experimental Brain Trauma: Overview, Research Applications, and Protocol. Methods Mol Biol. 2016;1462:177-92. doi: 10.1007/978-1-4939-3816-2_11. PMID: 27604719; PMCID: PMC5271598.
- Osier ND, Dixon CE. The Controlled Cortical Impact Model: Applications, Considerations for Researchers, and Future Directions. Front Neurol. 2016 Aug 17;7:134. doi: 10.3389/fneur.2016.00134. PMID: 27582726; PMCID: PMC4987613.
- Pareja JC, Keeley K, Duhaime AC, Dodge CP. Modeling Pediatric Brain Trauma: Piglet Model of Controlled Cortical Impact. Methods Mol Biol. 2016;1462:345-56. doi: 10.1007/978-1-4939-3816-2_19. PMID: 27604727.
- Schwerin SC, Hutchinson EB, Radomski KL, Ngalula KP, Pierpaoli CM, Juliano SL. Establishing the ferret as a gyrencephalic animal model of traumatic brain injury: Optimization of controlled cortical impact procedures. J Neurosci Methods. 2017 Jun 15;285:82-96. doi: 10.1016/j.jneumeth.2017.05.010. Epub 2017 May 10. PMID: 28499842; PMCID: PMC6320441.
- Schwulst SJ, Islam MBAR. Murine Model of Controlled Cortical Impact for the Induction of Traumatic Brain Injury. J Vis Exp. 2019 Aug 16;(150):10.3791/60027. doi: 10.3791/60027. PMID: 31475969; PMCID: PMC7046175.
- Sellappan P, Cote J, Kreth PA, Schepkin VD, Darkazalli A, Morris DR, Alvi FS, Levenson CW. Variability and uncertainty in the rodent controlled cortical impact model of traumatic brain injury. J Neurosci Methods. 2019 Jan 15;312:37-42. doi: 10.1016/j.jneumeth.2018.10.027. Epub 2018 Nov 10. PMID: 30423350.
- Siebold L, Obenaus A, Goyal R. Criteria to define mild, moderate, and severe traumatic brain injury in the mouse controlled cortical impact model. Exp Neurol. 2018 Dec;310:48-57. doi: 10.1016/j.expneurol.2018.07.004. Epub 2018 Jul 12. PMID: 30017882.
Fluid Percussion Model Protocol
- Rowe RK, Griffiths DR, Lifshitz J. Midline (Central) Fluid Percussion Model of Traumatic Brain Injury. Methods Mol Biol. 2016;1462:211-30. doi: 10.1007/978-1-4939-3816-2_13. PMID: 27604721.
- Van KC, Lyeth BG. Lateral (Parasagittal) Fluid Percussion Model of Traumatic Brain Injury. Methods Mol Biol. 2016;1462:231-51. doi: 10.1007/978-1-4939-3816-2_14. PMID: 27604722.
Blast Injury Model Protocol
- Heldt SA, Elberger AJ, Deng Y, Guley NH, Del Mar N, Rogers J, Choi GW, Ferrell J, Rex TS, Honig MG, Reiner A. A novel closed-head model of mild traumatic brain injury caused by primary overpressure blast to the cranium produces sustained emotional deficits in mice. Front Neurol. 2014 Jan 22;5:2. doi: 10.3389/fneur.2014.00002. PMID: 24478749; PMCID: PMC3898331.
- Guley NH, Rogers JT, Del Mar NA, Deng Y, Islam RM, D'Surney L, Ferrell J, Deng B, Hines-Beard J, Bu W, Ren H, Elberger AJ, Marchetta JG, Rex TS, Honig MG, Reiner A. A Novel Closed-Head Model of Mild Traumatic Brain Injury Using Focal Primary Overpressure Blast to the Cranium in Mice. J Neurotrauma. 2016 Feb 15;33(4):403-22. doi: 10.1089/neu.2015.3886. Epub 2015 Dec 17. PMID: 26414413; PMCID: PMC4761824.
Weight-Drop Model Protocol
- Büchele F, Morawska MM, Schreglmann SR, Penner M, Muser M, Baumann CR, Noain D. Novel Rat Model of Weight Drop-Induced Closed Diffuse Traumatic Brain Injury Compatible with Electrophysiological Recordings of Vigilance States. J Neurotrauma. 2016 Jul 1;33(13):1171-80. doi: 10.1089/neu.2015.4001. Epub 2015 Nov 13. PMID: 26414556.
- Chen Y, Mao H, Yang KH, Abel T, Meaney DF. A modified controlled cortical impact technique to model mild traumatic brain injury mechanics in mice. Front Neurol. 2014 Jun 18;5:100. doi: 10.3389/fneur.2014.00100. PMID: 24994996; PMCID: PMC4061598.
- Hellewell SC, Ziebell JM, Lifshitz J, Morganti-Kossmann MC. Impact Acceleration Model of Diffuse Traumatic Brain Injury. Methods Mol Biol. 2016;1462:253-66. doi: 10.1007/978-1-4939-3816-2_15. PMID: 27604723.
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