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Recent advances have made modeling human small intestines in vitro possible, but it remains a challenge to recapitulate fully their structural and functional characteristics. We suspected interstitial flow within the intestine, powered by circulating blood plasma during embryonic organogenesis, to be a vital factor. We aimed to construct an in vivo-like multilayered small intestinal tissue by incorporating interstitial flow into the system and, in turn, developed the micro-small intestine system by differentiating definitive endoderm and mesoderm cells from human pluripotent stem cells simultaneously on a microfluidic device capable of replicating interstitial flow. This approach enhanced cell maturation and led to the development of a three-dimensional small intestine-like tissue with villi-like epithelium and an aligned mesenchymal layer. Our micro-small intestine system not only overcomes the limitations of conventional intestine models but also offers a unique opportunity to gain insights into the detailed mechanisms underlying intestinal tissue development.
Pubmed ID: 38996472
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This monoclonal targets Vimentin (V9)
View all literature mentionsThis monoclonal targets Villin antibody [SP145]
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis monoclonal targets Villin antibody [EPR3490]
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis monoclonal targets CDX2
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Raised against amino acids 680-781 mapping at the C-terminus of b-Cat o human origin
View all literature mentionsThis polyclonal targets Chromogranin A antibody
View all literature mentionsThis monoclonal targets Lysozyme antibody [EPR2994(2)]
View all literature mentionsThis monoclonal targets Villin (1D2C3)
View all literature mentionsThis monoclonal targets Mucin 2
View all literature mentionsThis monoclonal targets slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH
View all literature mentionsThis polyclonal targets Collagen Type I
View all literature mentionsThis recombinant monoclonal targets Ki67
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsCell line L-WRN is a Spontaneously immortalized cell line with a species of origin Mus musculus (Mouse)
View all literature mentionsCell line MRC-5 is a Finite cell line with a species of origin Homo sapiens
View all literature mentions