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Publication

Endomucin marks quiescent long-term multi-lineage repopulating hematopoietic stem cells and is essential for their transendothelial migration.

Sophia Engelhard | Montserrat Estruch | Shuyu Qin | Christoph A Engelhard | Francisco G Rodriguez-Gonzalez | Martine Drilsvik | Javier Martin-Gonzalez | Jeng-Wei Lu | David Bryder | Claus Nerlov | Joachim Weischenfeldt | Kristian Reckzeh | Kim Theilgaard-Mönch
Cell reports | 2024

Endomucin (EMCN) currently represents the only hematopoietic stem cell (HSC) marker expressed by both murine and human HSCs. Here, we report that EMCN+ long-term repopulating HSCs (LT-HSCs; CD150+CD48-LSK) have a higher long-term multi-lineage repopulating capacity compared to EMCN- LT-HSCs. Cell cycle analyses and transcriptional profiling demonstrated that EMCN+ LT-HSCs were more quiescent compared to EMCN- LT-HSCs. Emcn-/- and Emcn+/+ mice displayed comparable steady-state hematopoiesis, as well as frequencies, transcriptional programs, and long-term multi-lineage repopulating capacity of their LT-HSCs. Complementary functional analyses further revealed increased cell cycle entry upon treatment with 5-fluorouracil and reduced granulocyte colony-stimulating factor (GCSF) mobilization of Emcn-/- LT-HSCs, demonstrating that EMCN expression by LT-HSCs associates with quiescence in response to hematopoietic stress and is indispensable for effective LT-HSC mobilization. Transplantation of wild-type bone marrow cells into Emcn-/- or Emcn+/+ recipients demonstrated that EMCN is essential for endothelial cell-dependent maintenance/self-renewal of the LT-HSC pool and sustained blood cell production post-transplant.

Pubmed ID: 38996072

Research resources used in this publication

Additional research tools detected in this publication

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Antibodies used in this publication

Associated grants

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Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.

Brilliant Violet 711(TM) anti-mouse CD150 (SLAM) (antibody)

RRID:AB_2629660

This monoclonal targets CD150

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Ki-67 Monoclonal Antibody (SolA15), FITC, eBioscience (antibody)

RRID:AB_11151330

This monoclonal targets Ki-67

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Alexa Fluor(R) 488 Goat anti-rat IgG (minimal x-reactivity) (antibody)

RRID:AB_2563120

This unknown targets IgG

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CD135 (FLT3) (antibody)

RRID:AB_2737639

This monoclonal targets CD135 (FLT3)

View all literature mentions

CD201 (EPCR) Monoclonal Antibody (eBio1560 (1560)), APC, eBioscience (antibody)

RRID:AB_10717805

This monoclonal targets CD201 (EPCR)

View all literature mentions

Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience (antibody)

RRID:AB_1834375

This isotype control targets Not Applicable

View all literature mentions

PE/Cyanine5 anti-mouse/human CD11b (antibody)

RRID:AB_312793

This monoclonal targets CD11b

View all literature mentions

FITC anti-mouse CD45.1 (antibody)

RRID:AB_313495

This monoclonal targets CD45.1

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FITC anti-mouse CD45.2 (antibody)

RRID:AB_313443

This monoclonal targets CD45.2

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CD11b Monoclonal Antibody (M1/70), PE-Cyanine7, eBioscience (antibody)

RRID:AB_469588

This monoclonal targets CD11b

View all literature mentions

PE/Cyanine5 anti-mouse TER-119/Erythroid Cells (antibody)

RRID:AB_313711

This monoclonal targets TER-119

View all literature mentions

PE/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) (antibody)

RRID:AB_313381

This monoclonal targets Ly-6G Ly-6C

View all literature mentions

CD117 (c-Kit) Monoclonal Antibody (2B8), APC-eFluor™ 780, eBioscience (antibody)

RRID:AB_1272177

This monoclonal targets CD117 (c-Kit)

View all literature mentions

CD45 Monoclonal Antibody (30-F11), PE-Cyanine5, eBioscience (antibody)

RRID:AB_468752

This monoclonal targets CD45

View all literature mentions

Brilliant Violet 650(TM) anti-mouse CD150 (SLAM) (antibody)

RRID:AB_2715765

This monoclonal targets CD150

View all literature mentions

PE/Cyanine5 anti-mouse Ly-6G/Ly-6C (Gr-1) (antibody)

RRID:AB_313375

This monoclonal targets Ly-6G Ly-6C

View all literature mentions

CD34 Monoclonal Antibody (RAM34), FITC, eBioscience (antibody)

RRID:AB_465022

This monoclonal targets CD34

View all literature mentions

CD31 (PECAM-1) Monoclonal Antibody (390), FITC, eBioscience (antibody)

RRID:AB_465012

This monoclonal targets CD31 (PECAM-1)

View all literature mentions

Endomucin Monoclonal Antibody (eBioV.7C7 (V.7C7)), PE, eBioscience (antibody)

RRID:AB_2784627

This monoclonal targets Endomucin

View all literature mentions

Endomucin Monoclonal Antibody (eBioV.7C7 (V.7C7)), eBioscience (antibody)

RRID:AB_891527

This monoclonal targets Endomucin

View all literature mentions

CD16/CD32 Monoclonal Antibody (93), Alexa Fluor™ 700, eBioscience (antibody)

RRID:AB_493994

This monoclonal targets CD16/CD32

View all literature mentions

Ig (antibody)

RRID:AB_393876

This polyclonal targets Ig

View all literature mentions

CD3e Monoclonal Antibody (145-2C11), PE-Cyanine5, eBioscience (antibody)

RRID:AB_468690

This monoclonal targets CD3e

View all literature mentions

PE Rat IgG2a, κ Isotype Ctrl Antibody (antibody)

RRID:AB_326530

This monoclonal secondary targets

View all literature mentions

Pacific Blue(TM) anti-mouse Ly-6A/E (Sca-1) (antibody)

RRID:AB_493273

This monoclonal targets Ly-6A/E

View all literature mentions

PE/Cyanine7 anti-mouse CD48 (antibody)

RRID:AB_2075049

This monoclonal targets CD48

View all literature mentions

APC anti-mouse CD45.1 (antibody)

RRID:AB_313503

This monoclonal targets CD45.1

View all literature mentions

PE anti-mouse CD3epsilon (antibody)

RRID:AB_312673

This monoclonal targets CD3epsilon

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C57BL/6NTac (organism)

RRID:IMSR_TAC:B6

Mus musculus with name C57BL/6NTac from IMSR.

View all literature mentions

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The PRECISE-TBI toolbox are a collection tools and resources to increase rigor and reproducibility with Traumatic Brain Injury within the PRE Clinical Interagency reSearch resourcE - Traumatic Brain Injury (PRECISE-TBI). Please learn more about PRECISE-TBI at https://www.precise-tbi.org

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