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A high-efficiency transient expression system mediated by Agrobacterium tumefaciens in Spinacia oleracea leaves.

Yumeng Zhang | Liuliu Qiu | Yongxue Zhang | Yiran Wang | Chunxiang Fu | Shaojun Dai | Meihong Sun
Plant methods | 2024

Optimization of a highly efficient transient expression system is critical for the study of gene function, particularly in those plants in which stable transformation methods are not widely available. Agrobacterium tumefaciens‑mediated transient transformation is a simple and low-cost method that has been developed and applied to a wide variety of plant species. However, the transient expression in spinach (Spinacia oleracea L.) is still not reported.

Pubmed ID: 38956683

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Associated grants

  • Agency: the National Natural Science Foundation of China,
    Id: No. 32070300
  • Agency: the Foundation of Shanghai Science and Technology Committee, China,
    Id: No.17391900600
  • Agency: the Fund of Shanghai Engineering Research Center of Plant Germplasm Resources,
    Id: No.17DZ2252700
  • Agency: the National Nature Science Foundation of China,
    Id: No.31902012
  • Agency: the Natural Science Foundation of Shanghai,
    Id: No. 22ZR1445700
  • Agency: Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education,
    Id: No. 0924210102

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Thermo Fisher Scientific (tool)

RRID:SCR_008452

Commercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.

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