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Clinical evaluation of cell-free and cellular metagenomic next-generation sequencing of infected body fluids.

Hongbin Chen | Yafeng Zheng | Xiaoyang Zhang | Si Liu | Yuyao Yin | Yifan Guo | Xiaojuan Wang | Yawei Zhang | Chunjiang Zhao | Wei Gai | Hui Wang
Journal of advanced research | 2024

Previous studies have evaluated metagenomic next-generation sequencing (mNGS) of cell-free DNA (cfDNA) for pathogen detection in blood and body fluid samples. However, no study has assessed the diagnostic efficacy of mNGS using cellular DNA.

Pubmed ID: 36889461

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This is a list of tools and resources that we have found mentioned in this publication.


Trimmomatic (tool)

RRID:SCR_011848

Software Java pipeline for trimming tasks for Illumina paired end and single ended data. Flexible Trimmer for Illumina Sequence Data. Pair aware preprocessing tool optimized for Illumina next generation sequencing data. Includes several processing steps for read trimming and filtering. Operating systems Unix/Linux, Mac OS, Windows.

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kraken2 (tool)

RRID:SCR_026838

Software tool as second version of Kraken taxonomic sequence classification system.

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