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Monitoring casbene synthase in Jatropha curcas tissues using targeted proteomics.

Natália Pinto de Almeida | Domingos Ferreira Mélo Neto | Gabriel Reis Alves Carneiro | Andreza Raquel Barbosa de Farias | Gilberto Barbosa Domont | Francisco de Assis de Paiva Campos | Fábio César Sousa Nogueira
Plant methods | 2021

Casbene synthase (CS) is responsible for the first committed step in the biosynthesis of phorbol esters (PE) in the Euphorbiaceae. PE are abundant in the seeds of the biofuel crop Jatropha curcas and its toxicity precludes the use of the protein-rich cake obtained after oil extraction as an animal feed and the toxicity of the fumes derived from burning PE containing biofuel is also a matter of concern. This toxicity is a major hindrance to exploit the potential of this crop as a source of raw material to produce biodiesel. For this reason, the current research on J. curcas is mainly focused on the understanding of the biosynthesis and site of synthesis of PE, as an avenue for the development of genotypes unable to synthesize PE in its seeds.

Pubmed ID: 33549129

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Skyline (tool)

RRID:SCR_014080

Software tool as Windows client application for targeted proteomics method creation and quantitative data analysis. Open source document editor for creating and analyzing targeted proteomics experiments. Used for large scale quantitative mass spectrometry studies in life sciences.

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Thermo Xcalibur (tool)

RRID:SCR_014593

A software which acquires and processes data sets, primarily through the Xcalibur system.

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