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Publication

Generation of six human induced pluripotent stem cell sublines (MZT01E, MZT01F, MZT01N and MZT02D, MZT02G and MZT02H) for reproductive science research.

Erica C Pandolfi | Enrique Sosa | Timothy J Hunt | Sierra Goldsmith | Kellie Hurlbut | Sherman J Silber | Amander T Clark
Stem cell research | 2021

Six human induced pluripotent stem cell sublines (hiPSCs) were generated from human dermal fibroblasts (HDFs) derived from skin biopsies donated from monozygotic twin women wherein one woman had proven fertility and her sister was infertile due to ovarian failure. Three hiPSC sublines were created from each twin's HDFs. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was further verified using PluriTest. We show here that the hiPSC lines created from the twins are equivalent in measures of pluripotency and self-renewal, despite their differential diagnosis.

Pubmed ID: 33548810

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.

Mouse Anti-Human Tra-1-85 Monoclonal antibody, Phycoerythrin Conjugated, Clone tra-1-85 (antibody)

RRID:AB_2066683

This monoclonal targets Human TRA-1-85

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Mouse Anti-Human SSEA-4 (carbohydrate from human embryonal carcinoma) Monoclonal Antibody, Unconjugated (antibody)

RRID:AB_528477

This monoclonal targets Mouse Human SSEA-4 (carbohydrate from human embryonal carcinoma)

View all literature mentions

Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)

RRID:AB_2535771

This polyclonal secondary targets IgG2a

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Alexa Fluor 488-AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot) (antibody)

RRID:AB_2340430

This unknown targets Goat IgG (H+L)

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Anti-Human TRA-1-81 Antibody, Clone TRA-1-81 (antibody)

RRID:AB_2721032

This monoclonal targets TRA-1-81

View all literature mentions

TRA-1-81 (Podocalyxin) Monoclonal Antibody (TRA-1-81), eBioscience (antibody)

RRID:AB_891614

This monoclonal targets TRA-1-81 (Podocalyxin)

View all literature mentions

Human/Mouse SSEA-4 Allophycocyanin MAb (Cl MC-813-70) (antibody)

RRID:AB_494994

This monoclonal targets Human/Mouse SSEA-4 Allophycocyanin MAb (Cl MC-813-70)

View all literature mentions

Human Nanog Antibody (antibody)

RRID:AB_355097

This polyclonal targets Nanog

View all literature mentions

Oct-3/4 (N-19) (antibody)

RRID:AB_653551

This polyclonal targets POU5F1

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The PRECISE-TBI toolbox are a collection tools and resources to increase rigor and reproducibility with Traumatic Brain Injury within the PRE Clinical Interagency reSearch resourcE - Traumatic Brain Injury (PRECISE-TBI). Please learn more about PRECISE-TBI at https://www.precise-tbi.org

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