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Rhodopsin is a light receptor comprised of an opsin protein and a light-sensitive retinal chromophore. Despite more than a century of scrutiny, there is no evidence that opsins function in chemosensation. Here, we demonstrate that three Drosophila opsins, Rh1, Rh4, and Rh7, are needed in gustatory receptor neurons to sense a plant-derived bitter compound, aristolochic acid (ARI). The gustatory requirements for these opsins are light-independent and do not require retinal. The opsins enabled flies to detect lower concentrations of aristolochic acid by initiating an amplification cascade that includes a G-protein, phospholipase Cβ, and the TRP channel, TRPA1. In contrast, responses to higher levels of the bitter compound were mediated through direct activation of TRPA1. Our study reveals roles for opsins in chemosensation and raise questions concerning the original roles for these classical G-protein-coupled receptors.
Pubmed ID: 32243853
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Cell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
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View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS05933}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; In(2LR)Gla, wg[Gla-1] PPO1[Bc]/CyO; TI{w[+mW.hs]=GAL4}TrpA1-CD TrpA1[-CD-G4] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)BSC730/TM6C, Sb[1] cu[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{y[+t7.7] w[+mC]=15XUAS-IVS-mCD8::GFP}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name cn[1] Galphaq[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3R)BSC636/TM6C, cu[1] Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; PBac{y[+mDint2] w[+mC]=hh-sfGFP}VK00037 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)Exel9003/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; Df(3L)ex83 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; sr[1] ninaE[17] e[s] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; TI{w[+mW.hs]=TI}TrpA1[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; sna[Sco]/CyO; P{w[+mC]=tubP-GAL80[ts]}ncd[GAL80ts-7] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=UAS-mCD8.mRFP.LG}18a from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; In(2LR)Gla, wg[Gla-1] PPO1[Bc]/CyO; TI{w[+mW.hs]=GAL4}TrpA1[-AB-G4] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; TI{w[+mW.hs]=GAL4}Gr33a[GAL4] from BDSC.
View all literature mentionsDrosophila melanogaster with name Rh6[1] from DGGR.
View all literature mentionsDrosophila melanogaster with name w[*]; P{y[+t7.7] w[+mC]=40XUAS-IVS-mCD8::GFP}attP2 from BDSC.
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View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF01438}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMC05183}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Gr5a-GAL4.8.5}6; Dr[1]/TM3, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; ninaD[1]/SM1 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{ry[+t7.2]=neoFRT}42D Df(2R)Galphaq1.3/CyO, P{y[+t7.7] ry[+t7.2]=Car20y}EW1 from BDSC.
View all literature mentionsDiscontinued
View all literature mentionsDrosophila melanogaster with name w[*] norpA[P24] from BDSC.
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View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Gr66a-GAL4.D}2; TI{w[+mW.hs]=GAL4}Gr93a[3] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
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