Searching the Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Extracellular vesicles (EVs) consist of lipid bilayers, occur in various biofluids, and are invaluable in biomarker screening. Liquid chromatography coupled with high-resolution mass spectrometry (LC-MS) was recently used to study comprehensive EV lipid profiles in vitro. The aim of this study was to establish a lipidomics platform for human plasma and serum EVs for comprehensive characterization of their lipid profiles, and to compare them with those of other lipid-containing particles, such as high-density lipoproteins (HDL), and low/very low-density lipoproteins (LDL/VLDL). Isolation was validated by specific protein markers; CD9 and MHC class for EVs, apoA-I for HDL, and apoB-100 for LDL/VLDL. Lipidomics identified 264 lipids from isolated plasma EVs, HDL, and LDL/VLDL. The absolute lipid levels per unit protein content in the EVs were more than eight times lower than those of the lipoproteins. Moreover, the EVs had higher lysoglycerophospholipid levels than HDL or LDL/VLDL. Similar profiles were also determined for human serum. The present study found that the lipid profiles of EVs are unique and distinctly different from those of lipoproteins. The lipidomics platform applied to human plasma and serum EVs could generate important information for the exploration and qualification of biomarkers in disease diagnosis.
Pubmed ID: 31683897
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Global nonprofit biological resource center (BRC) and research organization that provides biological products, technical services and educational programs to private industry, government and academic organizations. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement and application of scientific knowledge. The primary purpose of ATCC is to use its resources and experience as a BRC to become the world leader in standard biological reference materials management, intellectual property resource management and translational research as applied to biomaterial development, standardization and certification. ATCC characterizes cell lines, bacteria, viruses, fungi and protozoa, as well as develops and evaluates assays and techniques for validating research resources and preserving and distributing biological materials to the public and private sector research communities.
View all literature mentionsCommercial instrument and chemical vendor. Developer and manufacturer of specialized technological products for life science research and clinical diagnostics markets.
View all literature mentionsAs the integral bridge between researcher and patient, ProMedDx helps the world''s leading in-vitro diagnostic and research organizations fulfill the promise of disease eradication and healthier living. From a constantly evolving, high-grade Specimen Bank to a complete range of customized Clinical Services to a secure, state-of-the-art BioStorage, ProMedDx delivers the industry''s most trusted and comprehensive human biological solutions from collection to handling, storage, and transportation. Human biologics such as blood, plasma, urine, and tissue specimens possess crucial clues that can unlock the mystery of disease and lead to life altering treatments. As one of the most respected suppliers of human clinical specimens and patient samples, we are entrusted with a vital role in the advancement of medical research. ProMedDx is proud to further the scientific quest for discovery. That''s why we maintain an inventory of more than 1.8 million high quality healthy, and diseased human clinical specimens collected under IRB, ICH, and GCP guidelines and in adherence with HIPAA privacy regulations. Although our extensive Specimen Bank offers a wide ranging inventory, ProMedDx experts are also poised to help clients with clinical trial services or custom sample collections. ProMedDx has created one of the industry''s most secure, cost-efficient, and state-of-the-art specimen and tissue banks for for the storage of biological specimens. Strategically located between Boston, Massachusetts and Providence, Rhode Island, our on-site facility offers uncompromised Safety & Security. Best-in-class Technology runs our BioStorage facility and our centralized Logistics procedures have set new industry standards in packaging and shipping.
View all literature mentionsA commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentions