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Two subtractive cDNA libraries from banana leaves (cultivar 'Williams', genotype AAA) after biostimulant application on the leaf (library 1) or the substrate (library 2), with Pseudocercospora fijiensis infection were generated. The banana plants were applied first with the biostimulant and later the inoculation of P. fijiensis was performed on the leaves after one week. The suppression subtractive hybridization was performed by using as tester the treatments with biostimulant application by sampling banana leaves after two weeks of P. fijiensis inoculation, and every two weeks for two months (four time points); while the driver were collected on the same dates on independent banana plants that were only inoculated with P. fijiensis (no biostimulant application). The plants were maintained in the greenhouse for the entire assay.
Pubmed ID: 31656831
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