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Monitoring translation in synaptic fractions using a ribosome profiling strategy.

Konstanze Simbriger | Inês S Amorim | Kleanthi Chalkiadaki | Gilliard Lach | Seyed Mehdi Jafarnejad | Arkady Khoutorsky | Christos G Gkogkas
Journal of neuroscience methods | 2020

The aim of this study was to develop a method to study genome-wide local translation in biochemically isolated synaptic fractions (synaptoneurosomes). This methodology is of particular interest for neurons, due to the cardinal role of local translational control in neuronal sub-compartments, such as dendrites, for plasticity, learning, memory, and for disorders of the nervous system.

Pubmed ID: 31610213

Research resources used in this publication

None found

Antibodies used in this publication

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Associated grants

  • Agency: Wellcome Trust, United Kingdom
    Id: 107687/Z/15/Z

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


DAVID (tool)

RRID:SCR_001881

Bioinformatics resource system including web server and web service for functional annotation and enrichment analyses of gene lists. Consists of comprehensive knowledgebase and set of functional analysis tools. Includes gene centered database integrating heterogeneous gene annotation resources to facilitate high throughput gene functional analysis.

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New England Biolabs (tool)

RRID:SCR_013517

An Antibody supplier

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Agilent Technologies (tool)

RRID:SCR_013575

Company provides laboratories worldwide with analytical instruments and supplies, clinical and diagnostic testing services, consumables, applications and expertise in life sciences and applied chemical markets.

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C57BL/6J (tool)

RRID:IMSR_JAX:000664

Mus musculus with name C57BL/6J from IMSR.

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C57BL/6J (tool)

RRID:IMSR_JAX:000664

Mus musculus with name C57BL/6J from IMSR.

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