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Measurement of Myofilament-Localized Calcium Dynamics in Adult Cardiomyocytes and the Effect of Hypertrophic Cardiomyopathy Mutations.

Alexander J Sparrow | Kolja Sievert | Suketu Patel | Yu-Fen Chang | Connor N Broyles | Frances A Brook | Hugh Watkins | Michael A Geeves | Charles S Redwood | Paul Robinson | Matthew J Daniels
Circulation research | 2019

Subcellular Ca2+ indicators have yet to be developed for the myofilament where disease mutation or small molecules may alter contractility through myofilament Ca2+ sensitivity. Here, we develop and characterize genetically encoded Ca2+ indicators restricted to the myofilament to directly visualize Ca2+ changes in the sarcomere.

Pubmed ID: 30732532

Research resources used in this publication

None found

Antibodies used in this publication

None found

Associated grants

  • Agency: Wellcome Trust, United Kingdom
    Id: 098519
  • Agency: British Heart Foundation, United Kingdom
    Id: RE/13/1/30181
  • Agency: British Heart Foundation, United Kingdom
    Id: PG/18/68/33883
  • Agency: British Heart Foundation, United Kingdom
    Id: RG/18/9/33887
  • Agency: British Heart Foundation, United Kingdom
    Id: RG/12/16/29939
  • Agency: British Heart Foundation, United Kingdom
    Id: RE/08/004/23915

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Image Lab Software (tool)

RRID:SCR_014210

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KaleidaGraph (tool)

RRID:SCR_014980

Data analysis, graphing, and management application that allows users to import, manipulate, analyze data, and create customized plots. Plots include x-y probability, histogram, box, percentile, horizontal bar, stack bar, column, stack column, polar, and pie. Binned data can be exported to a histogram, step plot, or spike plot. KaleidaGraph works with Windows and Macintosh systems.

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Microsoft Excel (tool)

RRID:SCR_016137

Software application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.

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