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Metagenome Profiling Identifies Potential Biocontrol Agents for Selaginella kraussiana in New Zealand.

Zhenhua Dang | Patricia A McLenachan | Peter J Lockhart | Nick Waipara | Orhan Er | Christy Reynolds | Dan Blanchon
Genes | 2019

Metagenomics can be used to identify potential biocontrol agents for invasive species and was used here to identify candidate species for biocontrol of an invasive club moss in New Zealand. Profiles were obtained for Selaginella kraussiana collected from nine geographically disjunct locations in Northern New Zealand. These profiles were distinct from those obtained for the exotic club moss Selaginella moellendorffii and the native club mosses Lycopodium deuterodensum and Lycopodium volubile also collected in Northern New Zealand. Fungi and bacteria implicated elsewhere in causing plant disease were identified on plants of Selaginella that exhibited signs of necrosis. Most notably, high densities of sequence reads from Xanthomonas translucens and Pseudomonas syringae were associated with some populations of Selaginella but not Lycopodium. Since these bacteria are already in use as biocontrol agents elsewhere, further investigation into their potential as biocontrol of Selaginella in New Zealand is suggested.

Pubmed ID: 30709012

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This is a list of tools and resources that we have found mentioned in this publication.


SolexaQA (tool)

RRID:SCR_005421

Software package to calculate sequence quality statistics and create visual representations of data quality for Illumina's second-generation sequencing technology.

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DIAMOND (tool)

RRID:SCR_009457

Software to: view dicom files and assemble them into 3D volumes. View and convert between Analyze, Nifti, and Interfile. Classify and organize dicoms and 3D volumes using metadata. Search and report on a collection of scans.

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Trimmomatic (tool)

RRID:SCR_011848

Software Java pipeline for trimming tasks for Illumina paired end and single ended data. Flexible Trimmer for Illumina Sequence Data. Pair aware preprocessing tool optimized for Illumina next generation sequencing data. Includes several processing steps for read trimming and filtering. Operating systems Unix/Linux, Mac OS, Windows.

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MEGAN (tool)

RRID:SCR_011942

Software for analyzing metagenomes.

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Microsoft Excel (tool)

RRID:SCR_016137

Software application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.

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