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A novel method for cloning of coding sequences of highly toxic proteins.

Rafal Krela | Elzbieta Poreba | Martyna Weglewska | Tomasz Skrzypczak | Krzysztof Lesniewicz
Biochimica et biophysica acta. General subjects | 2019

During standard gene cloning, the recombinant protein appearing in bacteria as the result of expression leakage very often inhibits cell proliferation leading to blocking of the cloning procedure. Although different approaches can reduce transgene basal expression, the recombinant proteins, which even in trace amounts inhibit bacterial growth, can completely prevent the cloning process.

Pubmed ID: 30578833

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This is a list of tools and resources that we have found mentioned in this publication.


AxioVision Imaging System (tool)

RRID:SCR_002677

Digital image processing system where microscope settings and processing steps may be adjusted in single user interface. Can acquire images from variety of cameras. Includes software package for capturing, archiving and preparing images for publication. Allows users to visualize and present images in several dimensions. Functionality of imaging toolbox expands constantly with wide range of different modules that are tailored to specific applications or microscope accessories. This resource is duplicated by SCR_018376

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