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Blood vessel imaging using radiofrequency-induced second harmonic acoustic response.

Yuanhui Huang | Stephan Kellnberger | George Sergiadis | Vasilis Ntziachristos
Scientific reports | 2018

We introduce a contrast mechanism for visualizing blood vessels based on radiofrequency-induced second harmonic acoustic (RISHA) signals sensing blood conductivity. We develop a novel imaging system using commonly available inexpensive components, and demonstrate in vivo RISHA visualization of blood vessels based on low-power quasi-continuous radiofrequency excitation of tissue at frequencies of a few MHz. We show how the novel approach also implicitly enables radiofrequency-induced passive ultrasound imaging and can be readily applied to non-invasive imaging of blood vessels ex vivo and in vivo. We discuss the implications of non-invasive conductivity measurements in the context of biomedical applications.

Pubmed ID: 30341349

Research resources used in this publication

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Antibodies used in this publication

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Associated grants

  • Agency: Alexander von Humboldt-Stiftung (Alexander von Humboldt Foundation), International
    Id: Feodor Lynen Research Fellowship
  • Agency: Deutsche Forschungsgemeinschaft (German Research Foundation), International
    Id: Reinhard Kosselleck Award NT 3/9-1

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MATLAB (tool)

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Agilent Technologies (tool)

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Crl:CD1(ICR) (tool)

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Mus musculus with name Crl:CD1(ICR) from IMSR.

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