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Three-dimensional volume fluorescence-imaging of vascular plasticity in adipose tissues.

Ying Cao | Huanhuan Wang | Qi Wang | Xiangli Han | Wenwen Zeng
Molecular metabolism | 2018

The vascular system is central to sustaining tissue survival and homeostasis. Blood vessels are densely present in adipose tissues and exert essential roles in their metabolism. However, conventional immunohistochemistry methods have intrinsic limitations in examining the 3D vascular network in adipose tissues as well as other organs in general.

Pubmed ID: 29914852

Research resources used in this publication

Associated grants

None

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

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BioLegend (tool)

RRID:SCR_001134

Commercial antibody supplier and developer for biomedical research. These products are compatible with use in flow cytometry and mass cytometry, immunoprecipitation and chip, western blotting, immunofluorescence microscopy, and quantitative multiplexing.

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GraphPad Prism (tool)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

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Imaris (tool)

RRID:SCR_007370

Imaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.

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BD Biosciences (tool)

RRID:SCR_013311

An Antibody supplier

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C57BL/6J (tool)

RRID:IMSR_JAX:000664

Mus musculus with name C57BL/6J from IMSR.

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RFP Antibody Pre-adsorbed (antibody)

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Purified anti-mouse CD105 (antibody)

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Goat Anti-Human Perilipin A Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_944751

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FVB/N-Adrb3tm1Lowl/J (organism)

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RRID:IMSR_JAX:007914

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RRID:IMSR_JAX:003810

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B6;129S4-Gt(ROSA)26Sortm9(EGFP/Rpl10a)Amc/J (organism)

RRID:IMSR_JAX:024750

Mus musculus with name B6;129S4-Gt(ROSA)26Sortm9(EGFP/Rpl10a)Amc/J from IMSR.

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RRID:AB_390204

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Purified anti-mouse CD105 (antibody)

RRID:AB_961070

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RFP Antibody Pre-adsorbed (antibody)

RRID:AB_2209751

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RRID:IMSR_JAX:022362

Mus musculus with name B6.129P2(SJL)-Ntrk1tm1Ddg/J from IMSR.

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B6.Cg-Tg(Tek-cre)1Ywa/J (organism)

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RRID:IMSR_JAX:024750

Mus musculus with name B6;129S4-Gt(ROSA)26Sortm9(EGFP/Rpl10a)Amc/J from IMSR.

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B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (organism)

RRID:IMSR_JAX:007914

Mus musculus with name B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J from IMSR.

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Goat Anti-Human Perilipin A Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_944751

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RRID:IMSR_JAX:008863

Mus musculus with name B6.Cg-Tg(Tek-cre)1Ywa/J from IMSR.

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RRID:IMSR_JAX:008601

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RRID:IMSR_JAX:000697

Mus musculus with name B6.BKS(D)-Leprdb/J from IMSR.

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RRID:IMSR_JAX:000632

Mus musculus with name B6.Cg-Lepob/J from IMSR.

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STOCK Adrb1tm1Bkk Adrb2/J (organism)

RRID:IMSR_JAX:003810

Mus musculus with name STOCK Adrb1tm1Bkk Adrb2/J from IMSR.

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CD 31 Platelet Endothelial Cell Adhesion Molecule (PECAM) (antibody)

RRID:AB_394816

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