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An efficient and improved method for virus-induced gene silencing in sorghum.

Dharmendra Kumar Singh | Hee-Kyung Lee | Ismail Dweikat | Kirankumar S Mysore
BMC plant biology | 2018

Although the draft genome of sorghum is available, the understanding of gene function is limited due to the lack of extensive mutant resources. Virus-induced gene silencing (VIGS) is an alternative to mutant resources to study gene function. This study reports an improved and efficient method for Brome mosaic virus (BMV)-based VIGS in sorghum.

Pubmed ID: 29914379

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QIAGEN (tool)

RRID:SCR_008539

A commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.

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