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Transcriptomics and proteomics analyses of the PACAP38 influenced ischemic brain in permanent middle cerebral artery occlusion model mice.

Motohide Hori | Tomoya Nakamachi | Randeep Rakwal | Junko Shibato | Tetsuo Ogawa | Toshihiro Aiuchi | Tatsuaki Tsuruyama | Keiji Tamaki | Seiji Shioda
Journal of neuroinflammation | 2012

The neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) is considered to be a potential therapeutic agent for prevention of cerebral ischemia. Ischemia is a most common cause of death after heart attack and cancer causing major negative social and economic consequences. This study was designed to investigate the effect of PACAP38 injection intracerebroventrically in a mouse model of permanent middle cerebral artery occlusion (PMCAO) along with corresponding SHAM control that used 0.9% saline injection.

Pubmed ID: 23176072

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MatrixScience (tool)

RRID:SCR_000307

A collection of both commercial and noncommercial software products which includes: Mascot Distiller, Mascot Parser, and Mascot Server. Mascot Distiller is commercial and provides a single interface to process raw data into de-isotoped peak lists. This tool can also be used for the easy distribution of search and quantitative results to colleagues. The non-commercial Mascot Parser software provides an API (Application Programmer Interface) that makes it easier to access search results written in C++, Java, Python and Perl. Mascot Server is non-commercial, and is a collection of peptide mass fingerprints as well as a MS/MS database. A selection of popular sequence databases are available online and include SwissProt, NCBInr, and the EST divisions of EMBL. This server is best used for evaluating and searching for smaller data sets.

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NCBI (tool)

RRID:SCR_006472

A portal to biomedical and genomic information. NCBI creates public databases, conducts research in computational biology, develops software tools for analyzing genome data, and disseminates biomedical information for the better understanding of molecular processes affecting human health and disease.

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SABiosciences (tool)

RRID:SCR_008578

SABiosciences, as part of QIAGEN, offers nucleic acid (DNA, RNA, miRNA) and protein isolation, preparation and purification services, as well as assay,array, sequencing, NGS and biomarker services and lab essentials.Offers PCR services, assays, array systems, and expression vectors. Acquired by QIAGEN in 2009.

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C57BL/6J (tool)

RRID:IMSR_JAX:000664

Mus musculus with name C57BL/6J from IMSR.

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