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Comparison of total and cytoplasmic mRNA reveals global regulation by nuclear retention and miRNAs.

Beata Werne Solnestam | Henrik Stranneheim | Jimmie Hällman | Max Käller | Emma Lundberg | Joakim Lundeberg | Pelin Akan
BMC genomics | 2012

The majority of published gene-expression studies have used RNA isolated from whole cells, overlooking the potential impact of including nuclear transcriptome in the analyses. In this study, mRNA fractions from the cytoplasm and from whole cells (total RNA) were prepared from three human cell lines and sequenced using massive parallel sequencing.

Pubmed ID: 23110385

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U-251MG (tool)

RRID:CVCL_0021

Cell line U-251MG is a Cancer cell line with a species of origin Homo sapiens (Human)

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A-431 (tool)

RRID:CVCL_0037

Cell line A-431 is a Cancer cell line with a species of origin Homo sapiens (Human)

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