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Publication

A novel approach for transcription factor analysis using SELEX with high-throughput sequencing (TFAST).

Daniel J Reiss | Frederick M Howard | Harry L T Mobley
PloS one | 2012

In previous work, we designed a modified aptamer-free SELEX-seq protocol (afSELEX-seq) for the discovery of transcription factor binding sites. Here, we present original software, TFAST, designed to analyze afSELEX-seq data, validated against our previously generated afSELEX-seq dataset and a model dataset. TFAST is designed with a simple graphical interface (Java) so that it can be installed and executed without extensive expertise in bioinformatics. TFAST completes analysis within minutes on most personal computers.

Pubmed ID: 22956994

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Associated grants

  • Agency: NIAID NIH HHS, United States
    Id: T32 AI007528
  • Agency: NIAID NIH HHS, United States
    Id: AI59722
  • Agency: NIGMS NIH HHS, United States
    Id: T32 GM007863
  • Agency: NIAID NIH HHS, United States
    Id: AI007528
  • Agency: NIAID NIH HHS, United States
    Id: R01 AI059722
  • Agency: NIAID NIH HHS, United States
    Id: R56 AI043363
  • Agency: NIAID NIH HHS, United States
    Id: R01 AI043363
  • Agency: NIAID NIH HHS, United States
    Id: AI43363
  • Agency: NIGMS NIH HHS, United States
    Id: T32 GM07863

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