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Relative quantification is a commonly used method for assessing gene expression, however its accuracy and reliability is dependent upon the choice of an optimal endogenous control gene, and such choice cannot be made a priori. There is limited information available on suitable reference genes to be used for studies involving human epicardial adipose tissue. The objective of the current study was to evaluate and identify optimal reference genes for use in the relative quantification of gene expression in human epicardial fat depots of lean, overweight and obese subjects.
Pubmed ID: 22511915
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A public database holding PCR primers for popular DNA methylation analysis methods to prevent time-consuming primer design and experimental optimisation.
View all literature mentionsSoftware to determine most stable reference (housekeeping) genes from set of tested candidate reference genes in given sample panel. From this, gene expression normalization factor can be calculated for each sample based geometric mean of user-defined number of reference genes.
View all literature mentionsDesktop software application for bacterial identification, pathogen detection, or species identification. It can be used for cross-species identification for microarrays and real time PCR primers. It uses ClustalW to align sequences then analyzes conserved and species specific regions within those sequences.
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