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Validation of reference genes for expression analysis in the salivary gland and the intestine of Rhodnius prolixus (Hemiptera, Reduviidae) under different experimental conditions by quantitative real-time PCR.

Rafaela M Paim | Marcos H Pereira | Raffaello Di Ponzio | Juliana O Rodrigues | Alessandra A Guarneri | Nelder F Gontijo | Ricardo N Araújo
BMC research notes | 2012

Rhodnius prolixus is a blood-feeding insect that can transmit Trypanosoma cruzi and Trypanosoma rangeli to vertebrate hosts. Recently, genomic resources for invertebrate vectors of human pathogens have increased significantly, and R. prolixus has been one of the main species studied among the triatomines. However, the paucity of information on many of the fundamental molecular aspects of this species limits the use of the available genomic information. The present study aimed to facilitate gene expression studies by identifying the most suitable reference genes for the normalization of mRNA expression data from qPCR.

Pubmed ID: 22395020

Research resources used in this publication

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This is a list of tools and resources that we have found mentioned in this publication.


BLAST Assembled RefSeq Genomes (tool)

RRID:SCR_008420

This portal takes you to the NCBI''s BLAST Assembled RefSeq Genomes. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families. Sponsors: This resource is supported by the National Institutes of Health. Keywords: BLAST, Genome, Search engine, Sequence, Biological, Local, Alignment, Nucleotide, Protein, Program, Database, Stastical, Functional, Evolutionaary, Gene,

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Genome Institue at Washington University (tool)

RRID:SCR_010259

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on March 17, 2022. Genome databases housed at the Genome Institute at Washington University. Included are genome databases from Humans/Primates, other vertebrates, microorganisms, plants and invertebrates.

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Primer3 (tool)

RRID:SCR_003139

Tool used to design PCR primers from DNA sequence - often in high-throughput genomics applications. It does everything from mispriming libraries to sequence quality data to the generation of internal oligos.

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Consed (tool)

RRID:SCR_005650

A graphical tool for sequence finishing (BAM File Viewer, Assembly Editor, Autofinish, Autoreport, Autoedit, and Align Reads To Reference Sequence)

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geNORM (tool)

RRID:SCR_006763

Software to determine most stable reference (housekeeping) genes from set of tested candidate reference genes in given sample panel. From this, gene expression normalization factor can be calculated for each sample based geometric mean of user-defined number of reference genes.

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