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Standard colonic lavage alters the natural state of mucosal-associated microbiota in the human colon.

Laura Harrell | Yunwei Wang | Dionysios Antonopoulos | Vincent Young | Lev Lichtenstein | Yong Huang | Stephen Hanauer | Eugene Chang
PloS one | 2012

Past studies of the human intestinal microbiota are potentially confounded by the common practice of using bowel-cleansing preparations. We examined if colonic lavage changes the natural state of enteric mucosal-adherent microbes in healthy human subjects.

Pubmed ID: 22389708

Research resources used in this publication

None found

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Antibodies used in this publication

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Associated grants

  • Agency: NIDDK NIH HHS, United States
    Id: R37 DK47722
  • Agency: NIDDK NIH HHS, United States
    Id: P30 DK042086
  • Agency: NHGRI NIH HHS, United States
    Id: R21 HG004858
  • Agency: NIDDK NIH HHS, United States
    Id: DK-42086
  • Agency: NIDDK NIH HHS, United States
    Id: UH3 DK083993
  • Agency: NIDDK NIH HHS, United States
    Id: R37 DK047722
  • Agency: NIDDK NIH HHS, United States
    Id: UH2 DK083993
  • Agency: NHGRI NIH HHS, United States
    Id: HG004858
  • Agency: NIDDK NIH HHS, United States
    Id: DK083993

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GeneMapper (tool)

RRID:SCR_014290

Genotyping software package that provides DNA sizing and quality allele calls for all Applied Biosystems electrophoresis-based genotyping systems. GeneMapper specializes in multiapplication functionality, including amplified fragment length polymorphism, loss of heterozygosity, microsatellite, and SNP genotyping analysis. The software provides remote auto-analysis and command line operation, and allows for multiuser, client-server deployment.

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Simrank (tool)

RRID:SCR_014614

A rapid and sensitive general-purpose k-mer search tool.

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Unifrac (tool)

RRID:SCR_014616

THIS RESOURCE IS NO LONGER IN SERVICE, documented Setember 8, 2016. A suite of tools for the comparison of microbial communities using phylogenetic information. It takes as input a single phylogenetic tree that contains sequences derived from at least two different environmental samples and a file describing which sequences came from which sample.

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