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Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation.

Adila A Hamid | Ruszymah Bt Hj Idrus | Aminuddin Bin Saim | Somasumdaram Sathappan | Kien-Hui Chua
Clinics (Sao Paulo, Brazil) | 2012

Understanding the changes in chondrogenic gene expression that are involved in the differentiation of human adipose-derived stem cells to chondrogenic cells is important prior to using this approach for cartilage repair. The aims of the study were to characterize human adipose-derived stem cells and to examine chondrogenic gene expression after one, two, and three weeks of induction.

Pubmed ID: 22358233

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Primer3 (tool)

RRID:SCR_003139

Tool used to design PCR primers from DNA sequence - often in high-throughput genomics applications. It does everything from mispriming libraries to sequence quality data to the generation of internal oligos.

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LSU AgCenter Biotechnology Laboratory (tool)

RRID:SCR_012536

The LSU AgCenter Biotechnology Laboratory (ABL) is a core facility that provides basic and applied research expertise to support researchers in the LSU system as well as those in other academic institutions and industry. The ABL was formed in 1998 to provide support to faculty in performing biomolecular research. In 2010, the ABL was merged with the Protein Facility in LSU''''s College of Basic Sciences. Today, the ABL consists of three units: the Protein Facility, the Plant Transformation Facility and the Animal Cell Culture Facility.

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