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The nuclear-encoded chloroplast NADP-dependent malate dehydrogenase (NADP-MDH) is a key enzyme controlling the malate valve, to allow the indirect export of reducing equivalents. Arabidopsis thaliana (L.) Heynh. T-DNA insertion mutants of NADP-MDH were used to assess the role of the light-activated NADP-MDH in a typical C(3) plant. Surprisingly, even when exposed to high-light conditions in short days, nadp-mdh knockout mutants were phenotypically indistinguishable from the wild type. The photosynthetic performance and typical antioxidative systems, such as the Beck-Halliwell-Asada pathway, were barely affected in the mutants in response to high-light treatment. The reactive oxygen species levels remained low, indicating the apparent absence of oxidative stress, in the mutants. Further analysis revealed a novel combination of compensatory mechanisms in order to maintain redox homeostasis in the nadp-mdh plants under high-light conditions, particularly an increase in the NTRC/2-Cys peroxiredoxin (Prx) system in chloroplasts. There were indications of adjustments in extra-chloroplastic components of photorespiration and proline levels, which all could dissipate excess reducing equivalents, sustain photosynthesis, and prevent photoinhibition in nadp-mdh knockout plants. Such metabolic flexibility suggests that the malate valve acts in concert with other NADPH-consuming reactions to maintain a balanced redox state during photosynthesis under high-light stress in wild-type plants.
Pubmed ID: 22140244
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International functional genomics data collection generated from microarray or next-generation sequencing (NGS) platforms. Repository of functional genomics data supporting publications. Provides genes expression data for reuse to the research community where they can be queried and downloaded. Integrated with the Gene Expression Atlas and the sequence databases at the European Bioinformatics Institute. Contains a subset of curated and re-annotated Archive data which can be queried for individual gene expression under different biological conditions across experiments. Data collected to MIAME and MINSEQE standards. Data are submitted by users or are imported directly from the NCBI Gene Expression Omnibus.
View all literature mentionsA biology and bioinformatics laboratory at the Salk containing the following databases: SIGnAL T-DNA Express Arabidopsis Tiling Array Transcriptome Salk Insertion Sequence Database Arabidopsis Gene ORFeome Collection Salk Homozygote T-DNA Collection High Resolution Arabidopsis Methylome Perlegen Arabidopsis Resequencing Arabidopsis GMUCT Uncapped & Cleaved Transcripts Single Feature Polvmorphism Database High Resolution Arabidopsis Exosome Arabidopsis Interactome Single-base Resolution Epigenome Maps Transgenerational Inheritance of Methylation Variants Arabidopsis Cyclome Rice Functional Genomics Database Salk Arabidopsis 1,001 Genomes Arabidopsis Population Epigenomic Diversity Arabidopsis Biotic Stress Epigenome EIN3 -Ethylene Chip-seq/RNA-seq browser Soybean Epigenome Browser Human Human DNA Methylomes Human Pluripotent Cell Methylomes Human/Mouse Brain Methylomes Mouse Circadian Epigenome
View all literature mentionsSoftware package for the analysis of gene expression microarray data, especially the use of linear models for analyzing designed experiments and the assessment of differential expression.
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