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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00050754
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: lucEx825.
Alternate IDs:
Notes: lucEx825 [tbx-34::T2A::GFP::H2B::tbx-34 3'UTR + ttx-3p::mCherry]. Pick mCherry+ animals to maintain. Wild-type morphology. Extrachromosomal tbx-34 reporter includes 755 bp of tbx-34 upstream region and 4.4 kb of downstream region. Reference: Charest J, et al. Dev Cell. 2020 Sep 24;S1534-5807(20)30672-9. PMID: 33002421|"Made_by: Julien Charest"
Proper citation: RRID:WB-STRAIN:WBStrain00050754 Copy
http://www.wormbase.org/db/get?name=WBStrain00050753
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: lucEx824.
Alternate IDs:
Notes: lucEx824 [mab-9::T2A::GFP::H2B::mab-9 3'UTR + ttx-3p::mCherry]. Pick mCherry+ animals to maintain. Wild-type morphology. Extrachromosomal mab-9 reporter includes 3.1 kb of mab-9 upstream region and 1.5 kb of downstream region. Reference: Charest J, et al. Dev Cell. 2020 Sep 24;S1534-5807(20)30672-9. PMID: 33002421|"Made_by: Julien Charest"
Proper citation: RRID:WB-STRAIN:WBStrain00050753 Copy
http://www.wormbase.org/db/get?name=WBStrain00050740
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001078(dpy-19)|WBGene00001609(glp-1)|WBGene00006556(tbx-37)|WBGene00006557(tbx-38)
Genomic Alteration: WBGene00001078(dpy-19), WBGene00001609(glp-1), WBGene00006556(tbx-37), WBGene00006557(tbx-38)
Availability: unknown
References:
Synonyms: tbx-37(tm314) tbx-38(tm581)/qC1[dpy-19(e1259) glp-1(q339) qIs26] III.
Alternate IDs:
Notes: qIs26 [lag-2::GFP + rol-6(su1006)]. Heterozygote animals show roller phenotype and express GFP in the distal tip cells. Segregate roller and GFP(+) heterozygotes, embryonic lethal qC1 homozygotes and embryonic lethal tbx-37/38 homozygotes. Reference: Charest J, et al. Dev Cell. 2020 Sep 24;S1534-5807(20)30672-9. PMID: 33002421
Proper citation: RRID:WB-STRAIN:WBStrain00050740 Copy
http://www.wormbase.org/db/get?name=WBStrain00050744
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003280(mir-52)
Genomic Alteration: WBGene00003280(mir-52)
Availability: unknown
References:
Synonyms: nDf67 mir-52(n4100) IV/nT1 [qIs51] (IV;V); nDf58 X, lucIs24.
Alternate IDs:
Notes: lucIs24 [mir-52p::mirtron-51 + elt-2::dsRed + myo-2::mCherry]. Pick GFP+ animals to maintain balanced line. Balanced mir-51 family mutant expressing a mirtron-version of mir-51. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP, arrested nT1[qIs51] aneuploids, and non-GFP mir-51 family homozygous mutants. Pick wild-type GFP+ and check for correct segregation of progeny to maintain. Non-GFP mir-51 family homozygous mutants rescued by mirtron-51 transgene are viable, but slow-growing and sick. Strain is derived from injection into parental strain MT17143. lucIs24 is a spontaneous integrant originating from a complex extra-chromosomal array, the genomic location of the transgene is unknown. Reference: Dexheimer, PJ, et al. Curr Biol. 2020. in press.|"Made_by: Philipp Dexheimer"
Proper citation: RRID:WB-STRAIN:WBStrain00050744 Copy
http://www.wormbase.org/db/get?name=WBStrain00050748
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: lucSi100 II; unc-119(ed3) III.
Alternate IDs:
Notes: lucSi100 [hsp16.41::vhhGFP4::zif-1::SL2::mCherry::his-11::tbb-2 3'UTR] II. Superficially wild-type morphology. Single-copy insertion of a GFP-nanobody::zif-1 fusion transgene under a heat-shock promoter for timely controlled degradation of GFP-tagged proteins (Wang et al. (2017). A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans. Development 144, 2694-2701.)
Proper citation: RRID:WB-STRAIN:WBStrain00050748 Copy
http://www.wormbase.org/db/get?name=WBStrain00050747
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)|WBGene00011908(pash-1)
Genomic Alteration: WBGene00006843(unc-119), WBGene00011908(pash-1)
Availability: unknown
References:
Synonyms: pash-1(luc71[pash-1::2xGGSG::3xFLAG::AID::myc]) I; ieSi57 II; unc-119(ed3) III; ieSi38 IV.
Alternate IDs:
Notes: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Endogenous pash-1 tagged with the auxin-inducible-degron (AID) peptide at the C-terminus. Strain expresses modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and soma. Animals are superficially wild-type; addition of auxin induces embryonic lethality and larval arrest phenotypes. Reference: Dexheimer, PJ, et al. Curr Biol. 2020. in press.|"Made_by: Philipp Dexheimer"
Proper citation: RRID:WB-STRAIN:WBStrain00050747 Copy
http://www.wormbase.org/db/get?name=WBStrain00050738
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00000991(dhs-28)
Genomic Alteration: WBGene00000991(dhs-28)
Availability: unknown
References:
Synonyms: dhs-28(ldr6) X; ldrIs1; ldrIs2.
Alternate IDs:
Notes: ldrIs1 [dhs-3p::dhs-3::GFP + unc-76(+)]. ldrIs2 [mdt-28p::mdt-28::mCherry + unc-76(+)]. ldr6 is G-to-A causing a G158E substitution. Super-sized lipid droplets. [NOTE: The positions indicated in the original Figure 1C of Xie, et al. (2019) are based on an incorrect sequence map and do not reflect the position of the affected amino acid or position in a spliced transcript. The G158E substitution site of the ldr6 mutant is correct and has been independently confirmed by sequence analysis in another lab.] Reference: Xie K, et al. Sci Rep. 2019 Oct 17;9(1):14902. doi: 10.1038/s41598-019-51399-z. PMID: 31624276|"ldrIs1 [dhs-3p::dhs-3::GFP + unc-76(+)]. ldrIs2 [mdt-28p::mdt-28::mCherry + unc-76(+)]. ldr6 is G158A (Gly-Arg). Super-sized lipid droplets. Reference: Xie K, et al. Sci Rep. 2019 Oct 17;9(1):14902. doi: 10.1038/s41598-019-51399-z. PMID: 31624276"
Proper citation: RRID:WB-STRAIN:WBStrain00050738 Copy
http://www.wormbase.org/db/get?name=WBStrain00050843
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004010(pha-1)
Genomic Alteration: WBGene00004010(pha-1)
Availability: unknown
References:
Synonyms: pha-1(e2123) III; otEx7225.
Alternate IDs:
Notes: otEx7225 [eat-5(fosmid WRM0621dG04)::SL2::NLS::YFP::H2B + pha-1(+) + myo-2p::BFP]. Maintain at 25C or pick BFP+ to retain array. Reference: Bhattacharya A, et al. Cell. 2019 Feb 21;176(5):1174-1189.e16.
Proper citation: RRID:WB-STRAIN:WBStrain00050843 Copy
http://www.wormbase.org/db/get?name=WBStrain00050842
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: otIs439; hdIs32.
Alternate IDs:
Notes: Made_by: Oliver Hobert Lab|"otIs439 [lad-2p::GFP + pha-1(+)]. hdIs32 [glr-1::DsRed2]. SMD neurons are labeled with GFP and DsRed2. Can be used to isolate SMD by FACS. Used by CeNGEN project for RNA-Seq (https:"
Proper citation: RRID:WB-STRAIN:WBStrain00050842 Copy
http://www.wormbase.org/db/get?name=WBStrain00050841
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004010(pha-1)
Genomic Alteration: WBGene00004010(pha-1)
Availability: unknown
References:
Synonyms: pha-1(e2123) III; otEx7121.
Alternate IDs:
Notes: otEx7121 [inx-3(fosmid WRM0636dA10)::SL2::NLS::YFP::H2B + pha-1(+) + myo-2p::BFP]. Maintain at 25C or pick BFP+ to retain array. Reference: Bhattacharya A, et al. Cell. 2019 Feb 21;176(5):1174-1189.e16.
Proper citation: RRID:WB-STRAIN:WBStrain00050841 Copy
http://www.wormbase.org/db/get?name=WBStrain00050848
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004010(pha-1)
Genomic Alteration: WBGene00004010(pha-1)
Availability: unknown
References:
Synonyms: pha-1(e2123) III; otEx7292.
Alternate IDs:
Notes: otEx7292 [inx-7(fosmid WRM0631dH08)::SL2::NLS::YFP::H2B + pha-1(+) + myo-2p::BFP]. Maintain at 25C or pick BFP+ to retain array. Reference: Bhattacharya A, et al. Cell. 2019 Feb 21;176(5):1174-1189.e16.
Proper citation: RRID:WB-STRAIN:WBStrain00050848 Copy
http://www.wormbase.org/db/get?name=WBStrain00050847
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00002124(inx-2)
Genomic Alteration: WBGene00002124(inx-2)
Availability: unknown
References:
Synonyms: inx-2(ot906 [inx-2::SL2::NLS::yfp::H2B]) X.
Alternate IDs:
Notes: inx-2(ot906) was generated by the insertion of SL2::NLS::YFP::H2B into the endogenous inx-2 locus. Reference: Bhattacharya A, et al. Cell. 2019 Feb 21;176(5):1174-1189.e16.
Proper citation: RRID:WB-STRAIN:WBStrain00050847 Copy
http://www.wormbase.org/db/get?name=WBStrain00050846
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004010(pha-1)
Genomic Alteration: WBGene00004010(pha-1)
Availability: unknown
References:
Synonyms: pha-1(e2123) III; otEx7233.
Alternate IDs:
Notes: otEx7233 [inx-19(extended fosmid WRM0632bE10)::SL2::NLS::YFP::H2B + pha-1(+) + myo-2p::BFP]. Maintain at 25C or pick BFP+ to retain array. Reference: Bhattacharya A, et al. Cell. 2019 Feb 21;176(5):1174-1189.e16.
Proper citation: RRID:WB-STRAIN:WBStrain00050846 Copy
http://www.wormbase.org/db/get?name=WBStrain00050845
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004010(pha-1)
Genomic Alteration: WBGene00004010(pha-1)
Availability: unknown
References:
Synonyms: pha-1(e2123) III; otEx7232.
Alternate IDs:
Notes: otEx7232 [inx-15(fosmid WRM0619cH12)::SL2::NLS::YFP::H2B + pha-1(+) + myo-2p::BFP]. Maintain at 25C or pick BFP+ to retain array. Reference: Bhattacharya A, et al. Cell. 2019 Feb 21;176(5):1174-1189.e16.
Proper citation: RRID:WB-STRAIN:WBStrain00050845 Copy
http://www.wormbase.org/db/get?name=WBStrain00050849
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: otEx7294.
Alternate IDs:
Notes: otEx7294 [inx-8(fosmid WRM0632dA04)::SL2::NLS::YFP::H2B + rol-6(su1006)]. Pick Rollers to maintain.
Proper citation: RRID:WB-STRAIN:WBStrain00050849 Copy
http://www.wormbase.org/db/get?name=WBStrain00050838
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004010(pha-1)
Genomic Alteration: WBGene00004010(pha-1)
Availability: unknown
References:
Synonyms: pha-1(e2123) III; otEx7113.
Alternate IDs:
Notes: otEx7113 [inx-12(fosmid WRM0621dC07)::SL2::NLS::YFP::H2B + pha-1(+) + myo-2p::BFP]. Maintain at 25C or pick BFP+ to retain array. Reference: Bhattacharya A, et al. Cell. 2019 Feb 21;176(5):1174-1189.e16.
Proper citation: RRID:WB-STRAIN:WBStrain00050838 Copy
http://www.wormbase.org/db/get?name=WBStrain00050890
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00021911(affl-1)
Genomic Alteration: WBGene00021911(affl-1)
Availability: unknown
References:
Synonyms: Y55B1BR.1(sy1201) III.
Alternate IDs:
Notes: Made_by: Heenam Park/Mandy Tan|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of Y55B1BR.1; Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).Left flanking sequence: CCGTACCCGTAGAATGCTTGAAGAAATGGCCGGCCRight flanking sequence: TCGTGGGAACTAAACCATTGAGCCAGCTTCCTCGAAGinserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : TGAAGAAATGGCCGGCCTCGMethod Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00050890 Copy
http://www.wormbase.org/db/get?name=WBStrain00050895
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: nlp-77(sy1216) II.
Alternate IDs:
Notes: Made_by: Heenam Park/Mandy Tan|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of nlp-77; Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).Left flanking sequence: GACAACCAGCCGGAGGTCAAGATGTTCCACCATTCRight flanking sequence: CTTCGTAATGCCACTCCAGCTCAACTTCAGAGCTTCinserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : GGAGTGGCATTACGAAGGAAMethod Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00050895 Copy
http://www.wormbase.org/db/get?name=WBStrain00050894
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00045386(nlp-76)
Genomic Alteration: WBGene00045386(nlp-76)
Availability: unknown
References:
Synonyms: nlp-76(sy1214) X.
Alternate IDs:
Notes: Made_by: Heenam Park/Mandy Tan|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of nlp-76; Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).Left flanking sequence: cagTGTTCATCGCAATCTGCGTGCTCTCCCAAAARight flanking sequence: CGCTATGGCCCTCCGTGGTGCACTATTCCGTTCTGinserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : CACGGAGGGCCATAGCGTTTMethod Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00050894 Copy
http://www.wormbase.org/db/get?name=WBStrain00050892
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00022337(pals-16)
Genomic Alteration: WBGene00022337(pals-16)
Availability: unknown
References:
Synonyms: pals-16(sy1207) III.
Alternate IDs:
Notes: Made_by: Heenam Park/Mandy Tan|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of pals-16; Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).Left flanking sequence: GGAATCATTGACAAATTGCAGAACATCAACACCTTRight flanking sequence: Ggtaggttgaagaagttattattggaatttgaaatinserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : CAGAACATCAACACCTTGGTMethod Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00050892 Copy
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