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http://www.wormbase.org/db/get?name=WBStrain00051949

Source Database: WormBase (WB)
Affected Genes: WBGene00002245(lag-1)
Genomic Alteration: WBGene00002245(lag-1)
Availability: unknown
Source References: EMPTY
Synonyms: lag-1(st12314[lag-1::TY1::EGFP::3xFLAG]) IV.
Notes: CRISPR/Cas9 engineered tagged endogenous locus.|"Made_by: DKV/EF"

Proper citation: RRID:WB-STRAIN:WBStrain00051949 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052067

Source Database: WormBase (WB)
Affected Genes: WBGene00001332(eri-1)|WBGene00011333(nrde-2)
Genomic Alteration: WBGene00001332(eri-1), WBGene00011333(nrde-2)
Availability: unknown
Source References: EMPTY
Synonyms: vbaIs56 I; nrde-2(gg95) vbaIs55 II; eri-1(mg366) IV.
Notes: vbaIs56 [eef-1A.1p::VenusN::nrde-3] I. vbaIs55 [eef-1A.1p::VenusC::nrde-3] II. Maintain at 20C or cooler; germline mortal (Mrt) at 25C. N-terminal and C-terminal fragments of the fluorescent protein Venus are fused to NRDE-3 to facilitate trimolecular fluorescence complementation. In the cytoplasm or nucleus, local concentration of NRDE-3 molecules does not allow fluorescence complementation, thus reducing background fluorescence; once bound on the target transcript, VenusN::NRDE-3 and VenusC::NRDE-3 are in sufficient proximity to allow for fluorescence complementation, labeling transcription sites of dsRNA gene targets. Superficially wild-type. Reference: Toudji-Zouaz A, et al. Nucleic Acids Research. 2021 Jun 9;gkab469. doi: 10.1093/nar/gkab469. PMID: 34107044.

Proper citation: RRID:WB-STRAIN:WBStrain00052067 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052100

Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00022193(ppfr-4)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00022193(ppfr-4)
Availability: unknown
Source References: EMPTY
Synonyms: ppfr-4(gk5917[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ III.
Notes: Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 2578 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Please refer to supporting documents linked to the strain name in the CGC Strain Information display. Left flanking sequence: AAAAGTGATTTTGCGCCACTTTTTGAGCCT. Right flanking sequence: ACCAGCAACAAAGACAGTTCCAACACTTAA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"

Proper citation: RRID:WB-STRAIN:WBStrain00052100 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052068

Source Database: WormBase (WB)
Affected Genes: WBGene00001332(eri-1)|WBGene00011333(nrde-2)
Genomic Alteration: WBGene00001332(eri-1), WBGene00011333(nrde-2)
Availability: unknown
Source References: EMPTY
Synonyms: nrde-2(gg95) vbaIs54 II; eri-1(mg366) IV.
Notes: vbaIs54 [eef-1A.1p::YFP::nrde-3::SL2::sid-1] II. YFP::NRDE-3 localizes to the cytoplasm in most somatic tissues and upon exposure to dsRNA targetting a gene, moves to the nucleus in cells expressing the transgene. Superficially wild-type. Reference: Toudji-Zouaz A, et al. Nucleic Acids Research. 2021 Jun 9;gkab469. doi: 10.1093/nar/gkab469. PMID: 34107044.

Proper citation: RRID:WB-STRAIN:WBStrain00052068 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052061

Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:37934770
Synonyms: jsSi1579 jsSi1606 II.
Notes: jsSi1606 [loxP::unc-116(+)::FRT3] II. Single copy unc-116(+) insertion at the standard Chr II ttTi5605 mosSCI site. jsSi1579 is an RMCE landing pad inserted at a sgRNA site 45 bp from the ttTi5605 insertion site. It contains an rpl-28p::GFP reporter flanked by FRT and FRT3 sites and a loxP site (for more details about landing pads, see Nonet, 2020.Genetics or visit https:|"Supplementary_genotype jsSi1579 jsSi1606 [unc-116(+)] II"|"unc-116(+) single copy insertion."

Proper citation: RRID:WB-STRAIN:WBStrain00052061 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052063

Source Database: WormBase (WB)
Affected Genes: WBGene00003401(mpk-1)
Genomic Alteration: WBGene00003401(mpk-1)
Availability: unknown
Source References: EMPTY
Synonyms: narSi2 II; mpk-1(ga117) III.
Notes: narSi2 [mex-5p::GFP::mpk-1B + unc-119(+)] II. mpk-1(-) strain with germline-specific expression of GFP::MPK-1B. GFP::MPK-1B rescues fertility but the animals are still Vulvaless. Transgene uses codon-optimized version of GFP. Reference: Robinson-Thiewes et al. Cell Reports, In Press.

Proper citation: RRID:WB-STRAIN:WBStrain00052063 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052107

Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004151(pqn-68)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004151(pqn-68), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: pqn-68(gk5924[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP]) V.
Notes: Homozygous viable. Deletion of 925 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break. Please refer to supporting documents linked to the strain name in the CGC Strain Information display. Left flanking sequence: GAGTTGGTACATTTCTTGTGAAGGAGACCG. Right flanking sequence: AACTTTTGTCGATTTATGGATTTTGCATTA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"

Proper citation: RRID:WB-STRAIN:WBStrain00052107 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052108

Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: T26C5.4(gk5925[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ II.
Notes: Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 1296 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Please refer to supporting documents linked to the strain name in the CGC Strain Information display. Left flanking sequence: TAGCGGGGTATCTCCAGAGTTTGGATAATC. Right flanking sequence: CGGTGAAGCGAAGTGGGTGGATCTTTTTGA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"

Proper citation: RRID:WB-STRAIN:WBStrain00052108 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052109

Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: C16C10.4(gk5926[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP]) III.
Notes: Homozygous viable. Deletion of 897 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break. Please refer to supporting documents linked to the strain name in the CGC Strain Information display. Left flanking sequence: AAAGATTTGCGGTGAAAATCGAAATAAAAT. Right flanking sequence: AACGTAGTCCGATTCGCTAGACGCCTAACT. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"

Proper citation: RRID:WB-STRAIN:WBStrain00052109 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052102

Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: K11E4.2(gk5919[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP]) X.
Notes: Homozygous viable. Deletion of 1459 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break. Please refer to supporting documents linked to the strain name in the CGC Strain Information display. Left flanking sequence: TTGATTACTGTTATTTGGTAATGACTACCG. Right flanking sequence: GTGTTTTGAAACATCCAGTTGTGAGACGTC. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"

Proper citation: RRID:WB-STRAIN:WBStrain00052102 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052104

Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: T25G3.3(gk5921[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ I.
Notes: Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 2406 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Please refer to supporting documents linked to the strain name in the CGC Strain Information display. Left flanking sequence: TATATATTTTTTTTCAGTCAAAATGCAATC. Right flanking sequence: ACTCGCTTGTTGTTCTGTTATCACATTTTG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"

Proper citation: RRID:WB-STRAIN:WBStrain00052104 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052070

Source Database: WormBase (WB)
Affected Genes: WBGene00022608(madf-9)
Genomic Alteration: WBGene00022608(madf-9)
Availability: unknown
Source References: EMPTY
Synonyms: madf-9(gk3057) IV.
Notes: Made_by: Vancouver KO Group|"madf-9. Homozygous viable deletion, detectable by nested PCR. External left primer: AACAAAACGCATAACCTCCG. External right primer: TCGGCCAAACTTCATTTTTC. Internal left primer: AAAGAGAGAAGAGGGAGCCG. Internal right primer: GGCCAAATCTTTGTGGTTTG. Internal WT amplicon: 1269 bp. Deletion size: 784 bp. Deletion left flank: CCTCCCAACCGCACACATACTACCAACGAT. Deletion right flank: TTAAATTGAGAAATGAAAAAAAAGGTCACG. Validation: gk3057 confirmed by CGH."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."

Proper citation: RRID:WB-STRAIN:WBStrain00052070 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052071

Source Database: WormBase (WB)
Affected Genes: WBGene00022681(ZK185.1)
Genomic Alteration: WBGene00022681(ZK185.1)
Availability: unknown
Source References: EMPTY
Synonyms: ZK185.1(gk3229) IV.
Notes: Made_by: Vancouver KO Group|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"ZK185.1. Homozygous viable deletion, detectable by nested PCR. External left primer: TCCAAGTCAGCGCCTCTTAT. External right primer: AATTCAGCGAAAATGATCCG. Internal left primer: TTGGCGATGAACGTACCATA. Internal right primer: AATCGTGTAGCTGGTGGAGG. Internal WT amplicon: 1766 bp. Deletion size: 472 bp. Deletion left flank: TTCACAAAAAGTTAGATAAAAAGGTTGTGC. Deletion right flank: CAGCTAGTTTTTGTGCATTTTCTCAGATGT. Insertion sequence at break: GGCTAGTTTTTGTG. Validation: gk3229 confirmed by CGH."

Proper citation: RRID:WB-STRAIN:WBStrain00052071 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052056

Source Database: WormBase (WB)
Affected Genes: WBGene00006932(vps-34)
Genomic Alteration: WBGene00006932(vps-34)
Availability: unknown
Source References: EMPTY
Synonyms: vps-34(udn82) I.
Notes: Made_by: UDN Screening Center|"vps-34 [Y752Y] #1. Control edit for vps-34(udn80). StyI restriction site created by synonymous changes for ease for genotyping."

Proper citation: RRID:WB-STRAIN:WBStrain00052056 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052051

Source Database: WormBase (WB)
Affected Genes: WBGene00004268(rab-5)
Genomic Alteration: WBGene00004268(rab-5)
Availability: unknown
Source References: EMPTY
Synonyms: rab-5(udn11) I; udnSi38 II.
Notes: Made_by: UDN Screening Center|"udnSi38 [rab5p::rab-5] II. rab-5[D135D]. rab-5 Control edit #1 with a singlecopy of wild-type rab-5 integrated into chromosome II at ttTi5605 site (II: 0.77). Maintain at 20 degrees. Wild-type looking."

Proper citation: RRID:WB-STRAIN:WBStrain00052051 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052053

Source Database: WormBase (WB)
Affected Genes: WBGene00021461(nekl-1)
Genomic Alteration: WBGene00021461(nekl-1)
Availability: unknown
Source References: EMPTY
Synonyms: nekl-1(udn68) I.
Notes: Made_by: UDN Screening Center|"nekl-1 Variant edit H292Q. AvaII restriction site created by synonymous changes for ease of genotyping. Wild-type looking."

Proper citation: RRID:WB-STRAIN:WBStrain00052053 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052059

Source Database: WormBase (WB)
Affected Genes: WBGene00001078(dpy-19)|WBGene00001609(glp-1)|WBGene00006840(unc-116)
Genomic Alteration: WBGene00001078(dpy-19), WBGene00001609(glp-1), WBGene00006840(unc-116)
Availability: unknown
Source References: PMID:37934770
Synonyms: unc-116(gk5722udn86)/qC1 [dpy-19(e1259) glp-1(q339)] nIs189 III.
Notes: Made_by: UDN Screening Center|"nIs189 [myo-2::GFP] integrated in or near qC1. No recombination seen between nIs189 and qC1; fails to complemement all markers on qC1. Pick wild-type GFP+ to maintain. Heterozygotes are slightly dumpy GFP+ (pharynx), and segregate wild-type GFP+ heterozygotes, Dpy Sterile GFP+, and non-GFP udn45 homozygotes (larval arrest; very few escapers grow into Dumpy Unc adults). Variant edit allele T90I. TspRI restriction site created by synonymous changes for ease of genotyping. Derived from VC4653; selection cassette in VC4653 was removed, and then crossed with qC1 nIs189 [myo-2::GFP] banlancer."|"nIs189 [myo-2::GFP] integrated in or near qC1. No recombination seen between nIs189 and qC1; fails to complement all markers on qC1. Pick wild-type GFP+ to maintain. Heterozygotes are wild-type GFP+ (pharynx), and segregate wild-type GFP+ heterozygotes, Dpy Sterile GFP+ (qC1 homozygotes), and non-GFP gk5722udn86 homozygotes (larval arrest; few escapers). Derived from VC4653; selection cassette in VC4653 was removed, and then crossed with qC1 nIs189 [myo-2::GFP] balancer."|"Null deletion."|"Supplementary_genotype unc-116(gk5722udn86)/qC1 [dpy-19(e1259) glp-1(q339)] nIs189 III"

Proper citation: RRID:WB-STRAIN:WBStrain00052059 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052060

Source Database: WormBase (WB)
Affected Genes: WBGene00006784(unc-49)|WBGene00013422(popl-5)
Genomic Alteration: WBGene00006784(unc-49), WBGene00013422(popl-5)
Availability: unknown
Source References: EMPTY
Synonyms: popl-5(udn113)/tmC29 [unc-49(tmIs1259)] III.
Notes: Made_by: UDN Screening Center|"popl-5 [S99I]/tmC29 III. Variant edit. Lethal mutation balanced by tmC29. Balancer marked with myo-2p::GFP. Heterozygotes are WT with pharyngeal GFP, and segregate GFP+ heterozygotes, non-GFP popl-5 [S99I] homozygotes (larval lethal), and Unc GFP+ tmC29 homozygotes. Pick fertile wild-type GFP+ to maintain. NOTE: udn113 homozygotes are partially L3 larval lethal: some homozygotes can develop into sterile adults with protruding vulvae. Pick fertile GFP+ to maintain. AvaII site added in S99I allele for ease of genotyping. Reference: Huang et al. 2022. PMID: 35121658"|"popl-5 [S99I]/tmC29 III. Variant edit. Lethal mutation balanced by tmC29. Balancer marked with myo-2p::GFP. Heterozygotes are WT with pharyngeal GFP, and segregate GFP+ heterozygotes, non-GFP popl-5 [S99I] homozygotes (larval lethal), and Unc GFP+ tmC29 homozygotes. Pick fertile wild-type GFP+ to maintain. NOTE: udn113 homozygotes are partially L3 laval lethal: some homozygotes can develop into sterile adults with protruding vulvae. Pick fertile GFP+ to maintain. AvaII site added in S99I allele for ease of genotyping. Reference: Huang et al. 2022. PMID: 35121658"

Proper citation: RRID:WB-STRAIN:WBStrain00052060 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052043

Source Database: WormBase (WB)
Affected Genes: WBGene00001178(egl-9)|WBGene00002632(let-413)
Genomic Alteration: WBGene00001178(egl-9), WBGene00002632(let-413)
Availability: unknown
Source References: EMPTY
Synonyms: let-413(udn27)/tmC3[egl-9(tmIs1230)] V.
Notes: let-413 [L248P]. Variant edit. Homozygous lethal or sterile deletion balanced by tmC3. Heterozygotes are wild-type mCherry+ and segregate mCherry+ heterozygotes, udn27 homozygotes (arrest stage unknown), and mCherry+ tmC3 homozygotes (Unc-23 Lon-3). Pick viable fertile mCherry+ animals to maintain. ApoI-HF restriction site created by synonymous changes for ease of genotyping.|"Made_by: UDN Screening Center"

Proper citation: RRID:WB-STRAIN:WBStrain00052043 Copy   

•   Source: Integrated Animals

http://www.wormbase.org/db/get?name=WBStrain00052045

Source Database: WormBase (WB)
Affected Genes: WBGene00002632(let-413)
Genomic Alteration: WBGene00002632(let-413)
Availability: unknown
Source References: EMPTY
Synonyms: let-413(udn32) V.
Notes: let-413 [L173M]. Variant edit. DdeI restriction site created by synonymous changes for ease of genotyping. Wild-type looking.|"Made_by: UDN Screening Center"

Proper citation: RRID:WB-STRAIN:WBStrain00052045 Copy   

•   Source: Integrated Animals