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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00051649
Source Database: WormBase (WB)
Affected Genes: WBGene00021811(ral-1)
Genomic Alteration: WBGene00021811(ral-1)
Availability: unknown
Source References: EMPTY
Synonyms: ral-1(re218[mKate2::3xFlag::ral-1]) III.
Notes: Made_by: Hanna Shin|"Ubiquitous expression and localization to plasma membranes. Derived in an N2 background. TD185 5 -GCCGGAAGAGTGATGAACCC- 3 TD186 5 -TAATGAGCTCGGAGACCATGGC- 3TD187 5 -CGCACCTCATCATACATGAACTGC- 3 Reference: Shin H, et al. Cell Rep. 2018 Sep 4;24(10):2669-2681. PMID: 30184501"
Proper citation: RRID:WB-STRAIN:WBStrain00051649 Copy
http://www.wormbase.org/db/get?name=WBStrain00051639
Source Database: WormBase (WB)
Affected Genes: WBGene00006831(unc-104)
Genomic Alteration: WBGene00006831(unc-104)
Availability: unknown
Source References: PMID:37651423
Synonyms: wrdSi23 I; unc-104(knu973[unc-104::AID]) II.
Notes: Made_by: Cori K. Cahoon|"Supplementary_genotype wrdSi23 [eft-3p::TIR1:F2A:mTagBFP:tbb2 3' UTR:: loxP] I; unc-104(knu973[unc-104::AID]) II"|"wrdSi23 [eft-3p::TIR1:F2A:mTagBFP:tbb2 3' UTR:: loxP] I. wrdSi23 is inserted at ttTi4348 (I: -5.32 cM). Pan-somatic expression of TIR1 co-factor for AID, and expression of AID-tagged blue protein in somatic nuclei. Auxin-inducible degradation (AID) tag inserted at C-terminus of endogenous unc-104 locus by CRISPR/Cas9. Can be used for auxin-induced immobilization of worms for live imaging. References: Cahoon CK and Libuda DE. bioRxiv 2021.05.25.445686; doi: https:"
Proper citation: RRID:WB-STRAIN:WBStrain00051639 Copy
http://www.wormbase.org/db/get?name=WBStrain00051690
Source Database: WormBase (WB)
Affected Genes: WBGene00014204(ccdc-47)
Genomic Alteration: WBGene00014204(ccdc-47)
Availability: unknown
Source References: EMPTY
Synonyms: ccdc-47(utx31[mNG::3xFlag::ccdc-47]) III.
Notes: Made_by: Anita Rhodes (Glow Worms '20)|"N-terminal tag of CCDC-47 via CRISPR/Cas9 knock-in of mNeonGreen at ccdc-47 locus. Insertion verified by PCR, Sanger sequencing, and fluorescence. Left flank: 5' gttaaatcactcaatttcgggtcgttcacc 3'; Right flank: 5' ATGAAGATAGTATGGATTTTCCTAATATTC 3' (3 silent mutations); sgRNA: cgttcaccATGAAAATCGTC; Cas9/sgRNA plasmid: pGLOW13; mNG^SEC^3xFlag plasmid: pGLOW17; SEC insertion allele strain (balanced): GLW38."
Proper citation: RRID:WB-STRAIN:WBStrain00051690 Copy
http://www.wormbase.org/db/get?name=WBStrain00051694
Source Database: WormBase (WB)
Affected Genes: WBGene00002008(hsp-4)
Genomic Alteration: WBGene00002008(hsp-4)
Availability: unknown
Source References: EMPTY
Synonyms: hsp-4(utx39[hsp-4::mNG::3xFlag]) II.
Notes: C-terminal tag of HSP-4 via CRISPR/Cas9 knock-in of mNeonGreen at hsp-4 locus. Insertion verified by PCR and fluorescence. Left flank: 5' TCGGCCGGAGGACAAGGAGAACAAGCTTCTGAGGAGCCATCGGAGGATCATGATGAACTG 3' (1 silent mutation); Right flank: 5' TAAaatattaattgccttcaactacttgct 3'; sgRNA: CGTCTCCAAACTTTACTCGG; Cas9/sgRNA plasmid: pGLOW44; mNG^SEC^3xFlag plasmid: pGLOW61; SEC insertion allele strain: GLW46.|"Made_by: Quincy Martin (Glow Worms '21)"
Proper citation: RRID:WB-STRAIN:WBStrain00051694 Copy
http://www.wormbase.org/db/get?name=WBStrain00051693
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: ZK1058.9(utx37[ZK1058.9::mNG::3xFlag]) III
Notes: C-terminal tag of ZK1058.9 via CRISPR/Cas9 knock-in of mNeonGreen at ZK1058.9 locus. Insertion verified by PCR and fluorescence. Left flank: 5' AGCTCAACGGCTAGCTGGTCTCGTTATTAT 3' (1 silent mutation); Right flank: 5' TAAtgaattttcctccaacttttgtcctct 3'; sgRNA: AATAACGAGACCAGCTAG (18 bp); Cas9/sgRNA plasmid: pGLOW58; mNG^SEC^3xFlag plasmid: pGLOW68; SEC insertion allele strain: GLW44.|"Made_by: Dan Tatulescu (Glow Worms '21)"
Proper citation: RRID:WB-STRAIN:WBStrain00051693 Copy
http://www.wormbase.org/db/get?name=WBStrain00051692
Source Database: WormBase (WB)
Affected Genes: WBGene00011036(edc-3)
Genomic Alteration: WBGene00011036(edc-3)
Availability: unknown
Source References: EMPTY
Synonyms: edc-3(utx35[mNG::3xFlag::edc-3]) I.
Notes: Made_by: Diya Sharma (Glow Worms '21)|"N-terminal tag of EDC-3 via CRISPR/Cas9 knock-in of mNeonGreen at edc-3 locus. Insertion verified by PCR and fluorescence. Left flank: 5' ggttccaattcttctgatttcaaattaaaa 3'; Right flank: 5' ATGGATGACAAACTCATTGGAAGCGTCATCTCTACGGAGACAAAGGACGG 3' (7 silent mutations); sgRNA: ATATCAACCGAAACTAAAGA; Cas9/sgRNA plasmid: pGLOW81; mNG^SEC^3xFlag plasmid: pGLOW103; SEC insertion allele strain: GLW42."
Proper citation: RRID:WB-STRAIN:WBStrain00051692 Copy
http://www.wormbase.org/db/get?name=WBStrain00051691
Source Database: WormBase (WB)
Affected Genes: WBGene00003607(nhr-8)
Genomic Alteration: WBGene00003607(nhr-8)
Availability: unknown
Source References: EMPTY
Synonyms: nhr-8(utx33[mNG::3xFlag::nhr-8]) IV.
Notes: Made_by: Gillian Witten (Glow Worms '21)|"N-terminal tag of NHR-8 via CRISPR/Cas9 knock-in of mNeonGreen at nhr-8 locus. Insertion verified by PCR and Sanger sequencing. Left flank: 5' taatcactaaaacaaaaatttcgtcattcc 3'; Right flank: 5' ATGCCTTCGTCTTCTCCATCGATGGACGAG 3'; sgRNA: CGATGGAGAAGACGAAGGCA; Cas9/sgRNA plasmid: pGLOW55; mNG^SEC^3xFlag plasmid: pGLOW56; SEC insertion allele strain: GLW40."
Proper citation: RRID:WB-STRAIN:WBStrain00051691 Copy
http://www.wormbase.org/db/get?name=WBStrain00051698
Source Database: WormBase (WB)
Affected Genes: WBGene00004931(sod-2)
Genomic Alteration: WBGene00004931(sod-2)
Availability: unknown
Source References: EMPTY
Synonyms: sod-2(utx53[mScarlet::3xMyc::sod-2]) I.
Notes: Made_by: Param Kapoor (Glow Worms '21)|"N-terminal tag of SOD-2 via CRISPR/Cas9 knock-in of mScarlet at sod-2 locus. Insertion verified by PCR and fluorescence. Left flank: 5' attgaatattttaattatttgcagccgaaa 3'; Right flank: 5' ATGCTTCAAAACACGGTTCGCTGTGTCTCA 3 (1 silent mutation); sgRNA: AAGCTTTGAGACACAGCGAA; Cas9/sgRNA plasmid: pGLOW98; mScarlet^SEC^3xMyc plasmid: pGLOW111; SEC insertion allele strain: GLW64."
Proper citation: RRID:WB-STRAIN:WBStrain00051698 Copy
http://www.wormbase.org/db/get?name=WBStrain00051697
Source Database: WormBase (WB)
Affected Genes: WBGene00004143(pqn-59)
Genomic Alteration: WBGene00004143(pqn-59)
Availability: unknown
Source References: EMPTY
Synonyms: pqn-59(utx51[mNG::3xFlag::pqn-59]) I.
Notes: Made_by: Persephone Alicea (Glow Worms '20)|"N-terminal tag of PQN-59 via CRISPR/Cas9 knock-in of mNeonGreen at pqn-59 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gaccctttttatttataatttgttttcaga 3'; Right flank: 5' ATGGGTATTAAAGGCGACAAAAAAGCTACT 3 (1 silent mutation); sgRNA: TGCAAGTCGCGCTTGATCGC; Cas9/sgRNA plasmid: pGLOW23; mNG^SEC^3xFlag plasmid: pGLOW24; SEC insertion allele strain (balanced): GLW62."
Proper citation: RRID:WB-STRAIN:WBStrain00051697 Copy
http://www.wormbase.org/db/get?name=WBStrain00051730
Source Database: WormBase (WB)
Affected Genes: WBGene00001196(egl-30)
Genomic Alteration: WBGene00001196(egl-30)
Availability: unknown
Source References: EMPTY
Synonyms: egl-30(pe914) I.
Notes: Presumptive gain-of-function mutant shows defects in salt-food associative learning and olfactory learning. Reference: Tomioka M, et al. 2006 Sep 7;51(5):613-25. PMID: 16950159
Proper citation: RRID:WB-STRAIN:WBStrain00051730 Copy
http://www.wormbase.org/db/get?name=WBStrain00051696
Source Database: WormBase (WB)
Affected Genes: WBGene00000216(asp-3)
Genomic Alteration: WBGene00000216(asp-3)
Availability: unknown
Source References: EMPTY
Synonyms: asp-3(utx47[mNG::3xFlag::asp-3]) X.
Notes: Made_by: Stephen Pullman (Glow Worms '21)|"N-terminal tag of ASP-3 via CRISPR/Cas9 knock-in of mNeonGreen at asp-3 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gcgctgcttctcaattagtgataacgcacc 3'; Right flank: 5' ATGTCGGGCCGCGTTTTCCTTCTTCTGGCT 3'; sgRNA: tagtgataacgcaccATGT (19 bp); Cas9/sgRNA plasmid: pGLOW74; mNG^SEC^3xFlag plasmid: pGLOW96; SEC insertion allele strain: GLW58."
Proper citation: RRID:WB-STRAIN:WBStrain00051696 Copy
http://www.wormbase.org/db/get?name=WBStrain00051735
Source Database: WormBase (WB)
Affected Genes: WBGene00000898(daf-2)
Genomic Alteration: WBGene00000898(daf-2)
Availability: unknown
Source References: EMPTY
Synonyms: daf-2(pe1230) III.
Notes: Daf-c phenotype at 25C; normal development at 20C. Defects in salt-starve associative learning and olfactory learning. Reference: Ohno H, et al. Science. 2014 Jul 18;345(6194):313-7. PMID: 25035490
Proper citation: RRID:WB-STRAIN:WBStrain00051735 Copy
http://www.wormbase.org/db/get?name=WBStrain00051734
Source Database: WormBase (WB)
Affected Genes: WBGene00000913(daf-18)
Genomic Alteration: WBGene00000913(daf-18)
Availability: unknown
Source References: EMPTY
Synonyms: daf-18(pe408) IV.
Notes: pe408 suppresses the defects in salt-starve associative learning of casy-1(tm718). Reference: Ohno H, et al. Science. 2014 Jul 18;345(6194):313-7. PMID: 25035490
Proper citation: RRID:WB-STRAIN:WBStrain00051734 Copy
http://www.wormbase.org/db/get?name=WBStrain00051699
Source Database: WormBase (WB)
Affected Genes: WBGene00002230(klp-20)|WBGene00006960(xbx-1)|WBGene00017973(ift-81)
Genomic Alteration: WBGene00002230(klp-20), WBGene00006960(xbx-1), WBGene00017973(ift-81)
Availability: unknown
Source References: EMPTY
Synonyms: klp-20(cas447[klp-20::gfp]) III; xbx-1(cas502[xbx-1::tagRFP]) V; ift-81(cas498[ift-81::tagBFP]) X.
Notes: Constructed by crossing individual fluorescence knock-in worms. GFP inserted into the endogenous klp-20 gene at its C-terminus, tagRFP::3xFlag inserted into the endogenous xbx-1 gene at its C-terminus and tagBFP inserted into the endogenous ift-81 gene at its C-terminus by Cas9-triggered homologous recombination. GFP enriched at the proximal region (middle segment) of amphid or phasmid sensory cilia while red and blue fluorescence enriched along the whole cilia. Very weak fluorescence in the cell bodies of ciliated neurons.
Proper citation: RRID:WB-STRAIN:WBStrain00051699 Copy
http://www.wormbase.org/db/get?name=WBStrain00051732
Source Database: WormBase (WB)
Affected Genes: WBGene00004036(plc-1)
Genomic Alteration: WBGene00004036(plc-1)
Availability: unknown
Source References: EMPTY
Synonyms: plc-1(pe1238) X.
Notes: Made_by: Iwata R.|"Presumptive null allele of plc-1. Shows a preference for low salt concentrations. Reference: Kunitomo H, et al., 2013, Nat Commun. 2013;4:2210. doi: 10.1038/ncomms3210."
Proper citation: RRID:WB-STRAIN:WBStrain00051732 Copy
http://www.wormbase.org/db/get?name=WBStrain00051737
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: peIs2113.
Notes: Made_by: Jang Moon Sun|"peIs2113 [gcy-21p::mCaspase + tax-4p::NLS::YC2.60 + lin-44p::GFP]. Genetic ablation of ASG neurons by specific expression of caspase. tax-4p::YC2.60 facilitates calcium imaging. Reference: Jang MS, et al. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18673-18683. PMID: 31455735"
Proper citation: RRID:WB-STRAIN:WBStrain00051737 Copy
http://www.wormbase.org/db/get?name=WBStrain00051683
Source Database: WormBase (WB)
Affected Genes: WBGene00001073(dpy-11)|WBGene00006761(unc-24)|WBGene00008140(xpf-1)|WBGene00008346(C56A3.8)
Genomic Alteration: WBGene00001073(dpy-11), WBGene00006761(unc-24), WBGene00008140(xpf-1), WBGene00008346(C56A3.8)
Availability: unknown
Source References: EMPTY
Synonyms: xpf-1(e1487) II; unc-24(e138)/nt1[let (m435)] IV; dpy-11(e224) C56A3.8(t2055)/nt1[let (m435)] V.
Notes: him-9 is other name for xpf-1|"Made_by: Vancouver KO Group"|"Maternal effect lethal mutation linked to dpy-11(e224) and pseudolinked to unc-24(e138), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t2055 homozygotes that produce unfertilized oocytes. GE2886 is also homozygous for him-9(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation."|"Maternal effect lethal mutation linked to dpy-11(e224) and pseudolinked to unc-24(e138), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t2055 homozygotes that produce unfertilized oocytes. GE2886 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9."
Proper citation: RRID:WB-STRAIN:WBStrain00051683 Copy
http://www.wormbase.org/db/get?name=WBStrain00051682
Source Database: WormBase (WB)
Affected Genes: WBGene00001073(dpy-11)|WBGene00006761(unc-24)|WBGene00008140(xpf-1)|WBGene00020675(spe-51)
Genomic Alteration: WBGene00001073(dpy-11), WBGene00006761(unc-24), WBGene00008140(xpf-1), WBGene00020675(spe-51)
Availability: unknown
Source References: EMPTY
Synonyms: xpf-1(e1487) II; unc-24(e138) T22B11.1(t1786)/nt1[let (m435)] IV; dpy-11(e224)/nt1[let (m435)] V.
Notes: him-9 is other name for xpf-1|"Made_by: Vancouver KO Group"|"Temperature-sensitive maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1786 homozygotes that produce unfertilized oocytes at restrictive temperature. GE2827 is also homozygous for him-9(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation."|"Temperature-sensitive maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1786 homozygotes that produce unfertilized oocytes at restrictive temperature. GE2827 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9."
Proper citation: RRID:WB-STRAIN:WBStrain00051682 Copy
http://www.wormbase.org/db/get?name=WBStrain00051681
Source Database: WormBase (WB)
Affected Genes: WBGene00001073(dpy-11)|WBGene00006761(unc-24)|WBGene00008140(xpf-1)|WBGene00008346(C56A3.8)
Genomic Alteration: WBGene00001073(dpy-11), WBGene00006761(unc-24), WBGene00008140(xpf-1), WBGene00008346(C56A3.8)
Availability: unknown
Source References: EMPTY
Synonyms: xpf-1(e1487) II; unc-24(e138)/nt1[let (m435)] IV; dpy-11(e224) C56A3.8(t2029)/nt1[let (m435)] V.
Notes: him-9 is other name for xpf-1|"Made_by: Vancouver KO Group"|"Maternal effect lethal mutation linked to dpy-11(e224) and pseudolinked to unc-24(e138), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t2029 homozygotes that produce unfertilized oocytes. GE2734 is also homozygous for him-9(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation."|"Maternal effect lethal mutation linked to dpy-11(e224) and pseudolinked to unc-24(e138), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t2029 homozygotes that produce unfertilized oocytes. GE2734 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9."
Proper citation: RRID:WB-STRAIN:WBStrain00051681 Copy
http://www.wormbase.org/db/get?name=WBStrain00051686
Source Database: WormBase (WB)
Affected Genes: WBGene00001946(his-72)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00001946(his-72), WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: muIs252 II; unc-119(ed3) his-72(utx21[his-72::wrmScarlet11::3xMyc]) III.
Notes: Made_by: Ella DeMott (Glow Worms '20)|"muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. C-terminal tag of HIS-72 via CRISPR/Cas9 knock-in of wrmScarlet11 into endogenous his-72 locus. Genetic background: strain CF4582. Insertion verified by PCR and fluorescence. Left flank: 5' CTCGCCAGACGCATTCGCGGAGAACGTGCT 3' (one silent mutation); Right flank: 5' TAAgctccatcaccaattctcgaagcactt 3'; sgRNA: GAGCTTAAGCACGTTCTCCG; Cas9/sgRNA plasmid: pGLOW87; wrmScarlet11^SEC^3xMyc plasmid: pGLOW88; SEC insertion allele strain: GLW26"
Proper citation: RRID:WB-STRAIN:WBStrain00051686 Copy
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