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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00051551
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00019496(frpr-14)
Genomic Alteration: WBGene00019496(frpr-14)
Availability: unknown
References:
Synonyms: syEx1787 [Pnpr-9::frpr-14 cDNA::SL2::GFP, Pofm-1::RFP]; sy1301
Alternate IDs:
Notes: Batch Strain object requested via get_NS_ids.pl
Proper citation: RRID:WB-STRAIN:WBStrain00051551 Copy
http://www.wormbase.org/db/get?name=WBStrain00051550
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00019445(frpr-12)
Genomic Alteration: WBGene00019445(frpr-12)
Availability: unknown
References:
Synonyms: frpr-12(sy1581) V.
Alternate IDs:
Notes: Batch Strain object requested via get_NS_ids.pl|"Made_by: Heenam Park"|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of frpr-12. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: GATTTTCCAACTTCTAATGTTCAAAGTTACACCTTGA right flanking sequence: ATGAAGTGTTTTCAATGGTTATGCTACCAATTATT inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: CATTGAAAACACTTCATTCA Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00051550 Copy
http://www.wormbase.org/db/get?name=WBStrain00051554
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00019496(frpr-14)
Genomic Alteration: WBGene00019496(frpr-14)
Availability: unknown
References:
Synonyms: syEx1803 [Phlh-34::Cbr-frpr-14 cDNA::SL2::GFP, Pofm-1::RFP]; sy1301
Alternate IDs:
Notes: Batch Strain object requested via get_NS_ids.pl
Proper citation: RRID:WB-STRAIN:WBStrain00051554 Copy
http://www.wormbase.org/db/get?name=WBStrain00051552
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00019496(frpr-14)
Genomic Alteration: WBGene00019496(frpr-14)
Availability: unknown
References:
Synonyms: syEx1789 [Pfrpr-14::frpr-14 gDNA::SL2::GFP, Phlh-34::mCherry-H2B, Pofm-1::RFP]; sy1301
Alternate IDs:
Notes: Batch Strain object requested via get_NS_ids.pl
Proper citation: RRID:WB-STRAIN:WBStrain00051552 Copy
http://www.wormbase.org/db/get?name=WBStrain00051559
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00019496(frpr-14)
Genomic Alteration: WBGene00019496(frpr-14)
Availability: unknown
References:
Synonyms: flp-6(ok3056); frpr-14(sy1301)
Alternate IDs:
Notes: Batch Strain object requested via get_NS_ids.pl
Proper citation: RRID:WB-STRAIN:WBStrain00051559 Copy
http://www.wormbase.org/db/get?name=WBStrain00051558
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00019496(frpr-14)
Genomic Alteration: WBGene00019496(frpr-14)
Availability: unknown
References:
Synonyms: flp-3(ok3265); frpr-14(sy1301)
Alternate IDs:
Notes: Batch Strain object requested via get_NS_ids.pl
Proper citation: RRID:WB-STRAIN:WBStrain00051558 Copy
http://www.wormbase.org/db/get?name=WBStrain00051544
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00020319(npr-31)
Genomic Alteration: WBGene00020319(npr-31)
Availability: unknown
References:
Synonyms: npr-31(sy1360) II.
Alternate IDs:
Notes: Batch Strain object requested via get_NS_ids.pl|"Made_by: Heenam Park & Mandy Tan"|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of npr-31. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: ggaaaaactagttataaaaatgttcagATCCCTTA right flanking sequence: TGTCCAGAAGCTCCCCTTCAGATCGATTACGTGC inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: AGGGGAGCTTCTGGACATAA Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00051544 Copy
http://www.wormbase.org/db/get?name=WBStrain00051542
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00020727(npr-21)
Genomic Alteration: WBGene00020727(npr-21)
Availability: unknown
References:
Synonyms: npr-21(sy1309) X.
Alternate IDs:
Notes: Batch Strain object requested via get_NS_ids.pl|"Made_by: Heenam Park & Mandy Tan"|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of npr-21. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: CTGTGTATCCTACCTTTTTGTCTTTCTGGCCACTAT right flanking sequence: AATCGgtatgccaaggatctttgttcatattttta inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: GTCTTTCTGGCCACTATAAT Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00051542 Copy
http://www.wormbase.org/db/get?name=WBStrain00051548
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00009619(npr-6)
Genomic Alteration: WBGene00009619(npr-6)
Availability: unknown
References:
Synonyms: npr-6(sy1571) X.
Alternate IDs:
Notes: Batch Strain object requested via get_NS_ids.pl|"Made_by: Heenam Park"|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of npr-6. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: gtaatttttattttaaaaaaacacgtttcagAACC right flanking sequence: CCATGGAAATGGAATATTTCCGCCCATTCTTTATTTC inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: AAACACGTTTCAGAACCCCA Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00051548 Copy
http://www.wormbase.org/db/get?name=WBStrain00051546
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00007346(frpr-2)
Genomic Alteration: WBGene00007346(frpr-2)
Availability: unknown
References:
Synonyms: frpr-2(sy1364) X.
Alternate IDs:
Notes: Batch Strain object requested via get_NS_ids.pl|"Made_by: Heenam Park"|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of frpr-2. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: GTTATGCGTGAGTGTGAATGCCTTCATGAGCCGATA right flanking sequence: GAGGGGTACGCGGGAGTTGCCAATCTGgtaagtatac inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: ACTCCCGCGTACCCCTCTAT Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00051546 Copy
http://www.wormbase.org/db/get?name=WBStrain00051545
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00018728(frpr-8)
Genomic Alteration: WBGene00018728(frpr-8)
Availability: unknown
References:
Synonyms: frpr-8(sy1363)
Alternate IDs:
Notes: Batch Strain object requested via get_NS_ids.pl
Proper citation: RRID:WB-STRAIN:WBStrain00051545 Copy
http://www.wormbase.org/db/get?name=WBStrain00051549
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00019496(frpr-14)
Genomic Alteration: WBGene00019496(frpr-14)
Availability: unknown
References:
Synonyms: syEx1785 [Pfrpr-14::frpr-14 gDNA::SL2::GFP, Pnpr-9::mCherry-H2B, Pofm-1::RFP]; sy1301
Alternate IDs:
Notes: Batch Strain object requested via get_NS_ids.pl
Proper citation: RRID:WB-STRAIN:WBStrain00051549 Copy
http://www.wormbase.org/db/get?name=WBStrain00051652
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: unc-119(ox819 oxTi1126) III.
Alternate IDs:
Notes: oxTi1126 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + myo-2p::2xNLS::cyOFP::let-858 3'UTR + lox2272] III. Knock-in into previously modified unc-119(ox819) endogenous locus. Cas9 insertion marked with myo-2p::cyOFP (cyan-excitable Orange Fluorescent Protein), a long-Stokes-shift fluorescent protein that is spectrally separable from common green and red fluorophores. The Cas9 transgene was optimized for germline expression by including 4 large PATC-rich introns from smu-2. Lower activity than other Cas9 strains, but useful because Cas9, Cre, and unc-119 are in a single unit. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00051652 Copy
http://www.wormbase.org/db/get?name=WBStrain00051658
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: unc-119(ox819) III; W03F9.11(oxTi1121) V.
Alternate IDs:
Notes: oxTi1121 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + lox2272]) V. The Cas9 transgene was optimized for germline expression by including 4 large PATC-rich introns from smu-2. Inserted into W03F9.11. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00051658 Copy
http://www.wormbase.org/db/get?name=WBStrain00051656
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: W01A8.6(oxTi1128) I; unc-119(ox819) III.
Alternate IDs:
Notes: oxTi1128 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + myo-2p::2xNLS::cyOFP::let-858 3'UTR + lox2272]) I. Cas9 insertion marked with myo-2p::cyOFP (cyan-excitable Orange Fluorescent Protein), a long-Stokes-shift fluorescent protein that is spectrally separable from common green and red fluorophores. Inserted into W01A8.6. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00051656 Copy
http://www.wormbase.org/db/get?name=WBStrain00051641
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006831(unc-104)
Genomic Alteration: WBGene00006831(unc-104)
Availability: unknown
References:
Synonyms: reSi7 I; unc-104(knu973[unc-104::AID]) II.
Alternate IDs:
Notes: Made_by: Cori K. Cahoon|"reSi7 [rgef-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Neuronal-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in neuronal nuclei. Auxin-inducible degradation (AID) tag inserted at C-terminus of endogenous unc-104 locus by CRISPR/Cas9. Can be used for auxin-induced immobilization of worms for live imaging. Strain generated by crossing endogenously tagged unc-104::AID into DV3805. reSi7 is at -5.32 cM. References: Cahoon CK and Libuda DE. bioRxiv 2021.05.25.445686; doi: https:"
Proper citation: RRID:WB-STRAIN:WBStrain00051641 Copy
http://www.wormbase.org/db/get?name=WBStrain00051640
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006757(unc-18)
Genomic Alteration: WBGene00006757(unc-18)
Availability: unknown
References:
Synonyms: wrdSi23 I; unc-18(knu969[unc-18::AID]) X.
Alternate IDs:
Notes: Made_by: Cori K. Cahoon|"wrdSi23 [eft-3p::TIR1:F2A:mTagBFP:tbb2 3' UTR:: loxP] I. wrdSi23 is inserted at ttTi4348 (I: -5.32 cM). Pan-somatic expression of TIR1 co-factor for AID, and expression of AID-tagged blue protein in somatic nuclei. Auxin-inducible degradation (AID) tag inserted at C-terminus of endogenous unc-18 locus by CRISPR/Cas9. Can be used for auxin-induced immobilization of worms for live imaging. References: Cahoon CK and Libuda DE. bioRxiv 2021.05.25.445686; doi: https:"
Proper citation: RRID:WB-STRAIN:WBStrain00051640 Copy
http://www.wormbase.org/db/get?name=WBStrain00051646
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001946(his-72)|WBGene00003401(mpk-1)
Genomic Alteration: WBGene00001946(his-72), WBGene00003401(mpk-1)
Availability: unknown
References:
Synonyms: his-72(cp76[mNeonGreen::3xFlag::his-72]) mpk-1(re172[mpk-1::mKate2::3xFlag]) III.
Alternate IDs:
Notes: Green nuclei and ubiquitous cytosolic red expression, typically excluded from nuclei but with activity-dependent translocation into nuclei. Derived in an N2 background.C-terminally tagged mpk-1 is detectable by triplex PCR:mpk-1 genotyping FW: ACCAAAACAACCATGGGCTCGmpk-1 genotyping RV-1: GCTCCAAGTATGGGTGAGCCmpk-1 genotyping RV-2: GGTTCCCTCGTATGGCTTTCCReference: Neal R, et al. (2021). Nuclear translocation of tagged endogenous ERK/MPK-1 MAP Kinase denotes a subset of activation events in C. elegans development.|"Made_by: Neal Rasmussen"
Proper citation: RRID:WB-STRAIN:WBStrain00051646 Copy
http://www.wormbase.org/db/get?name=WBStrain00051645
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006751(unc-11)
Genomic Alteration: WBGene00006751(unc-11)
Availability: unknown
References:
Synonyms: unc-11(vg103[unc-11::GFP]) I.
Alternate IDs:
Notes: Superficially wild-type. Endogenously-tagged UNC-11::GFP is expressed in most, if not all, neurons, epithelial cells and coelomocytes, and should be visible in the nerve ring through a dissecting scope..
Proper citation: RRID:WB-STRAIN:WBStrain00051645 Copy
http://www.wormbase.org/db/get?name=WBStrain00051644
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00015814(oatr-1)
Genomic Alteration: WBGene00015814(oatr-1)
Availability: unknown
References:
Synonyms: orn-1(tm5454) III.
Alternate IDs:
Notes: Reference: Bailly AP, et al. Cell Rep. 2019 Oct 1;29(1):212-224.e8. doi: 10.1016/j.celrep.2019.08.070. PMID: 31577950
Proper citation: RRID:WB-STRAIN:WBStrain00051644 Copy
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