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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00051062
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: mikSi1 II; ltIs38.
Alternate IDs:
Notes: Made_by: Anna Christina Erpf|"mikSi1 [sas-4p::dendra2::sas-4] inserted into ttTi5605 II. ltIs38 [pie-1p::GFP::PH(PLC1delta1) + unc-119(+)]. Photoconvertable tagged SAS-4 protein. Reference: Erpf AC & Mikeladze-Dvali T. (2020). Tracking of centriole inheritance in C. elegans. microPublication Biology. 10.17912/micropub.biology.000256."
Proper citation: RRID:WB-STRAIN:WBStrain00051062 Copy
http://www.wormbase.org/db/get?name=WBStrain00051061
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004879(smg-1)
Genomic Alteration: WBGene00004879(smg-1)
Availability: unknown
References:
Synonyms: smg-1(gk761853) I.
Alternate IDs:
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00051061 Copy
http://www.wormbase.org/db/get?name=WBStrain00051067
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001803(lite-1)
Genomic Alteration: WBGene00001803(lite-1)
Availability: unknown
References:
Synonyms: lite-1(xu492) X.
Alternate IDs:
Notes: Light sensation defect; loss of light sensation. lite-1(xu492) is a 2701 bp deletion generated by CRISPR/Cas9-based gene editing using the Fire Lab protocol (Arribere et al., 2014). Left flanking sequence: 5 CGTAAAAAACAACATGCCACCAC Right flanking sequence: 5' GGCGGCCACCTACGCCAGTA. Primer sequences used to detect the deletion: Forward (flanking): 5 GAAGAAAAGGCGGTGCAAAC; Reverse (flanking): 5 GAAGCAACAAGACGATCTCC; Forward (internal): 5 ATGATCGCAAAAATCCTGTCGAGTC. Wild-type product: 1972 bp; xu492 product: 1475 bp; both bands should be visible if heterozygous. Reference: Zhang W, et al. PLoS Genet. 2020 Dec 10;16(12):e1009257. doi: 10.1371/journal.pgen.1009257. eCollection 2020 Dec.
Proper citation: RRID:WB-STRAIN:WBStrain00051067 Copy
http://www.wormbase.org/db/get?name=WBStrain00051100
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
References:
Synonyms: C34D4.2(gk3801[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP]) IV.
Alternate IDs:
Notes: Homozygous viable. Deletion of 800 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break. Left flanking sequence: TAATTAGTCAGTATTTTTACTTGCCAGACG. Right flanking sequence: CGGACAAAGTTTTCTTGCTCCGAGGAAATC. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051100 Copy
http://www.wormbase.org/db/get?name=WBStrain00051066
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: Y74C10AL.2(ogr3) I.
Alternate IDs:
Notes: Short-lived at 25C. Sensitive to paraquat. ogr3 is a 1238 bp deletion; flanking sequences: aaaattttttaaaaaaatat - taaaatcttccaacaaaaaaa
Proper citation: RRID:WB-STRAIN:WBStrain00051066 Copy
http://www.wormbase.org/db/get?name=WBStrain00051065
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: T09F3.2(ogr2) II.
Alternate IDs:
Notes: Slow growth, Slow movement. T09F3.2 encodes a homolog of human mitochondrial pyrimidine nucleotide transporter. ogr2 is a 718 bp deletion; flanking sequences: gagtcggaagttctaattccaaatt - atcagtctaaatatcatttttcttctt
Proper citation: RRID:WB-STRAIN:WBStrain00051065 Copy
http://www.wormbase.org/db/get?name=WBStrain00051102
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00019077(zipt-11)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00019077(zipt-11)
Availability: unknown
References:
Synonyms: zipt-11(gk3833[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP]) I.
Alternate IDs:
Notes: Homozygous viable. Deletion of 1674 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break. Left flanking sequence: AATTTATCAGGCGCTTCTTGCGGCCACATT. Right flanking sequence: GGGTTAAACTCGGAAACTTGTTCACAACCC. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051102 Copy
http://www.wormbase.org/db/get?name=WBStrain00051101
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
References:
Synonyms: eif-3.G(gk3804[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ II.
Alternate IDs:
Notes: Made_by: Vancouver KO Group|"[NOTE: Please see RG5004 for balanced version of this strain.] Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 717 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: TTTGTTATCCGATGGCCAAAAAATTCGCCT. Right flanking sequence: AATGATATCCGAATGTACCATATGGTTCTC. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation."
Proper citation: RRID:WB-STRAIN:WBStrain00051101 Copy
http://www.wormbase.org/db/get?name=WBStrain00051107
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
References:
Synonyms: C30E1.9(gk3854[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP]) X.
Alternate IDs:
Notes: Homozygous viable. Deletion of 11852 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break. Left flanking sequence: TGTTCCCTCACCCTTACTTTCGAATTCCCT. Right flanking sequence: TGGTTGTTTAATATGGTTATTCTTAAGGTA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051107 Copy
http://www.wormbase.org/db/get?name=WBStrain00051050
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00022296(xpc-1)
Genomic Alteration: WBGene00022296(xpc-1)
Availability: unknown
References:
Synonyms: xpc-1(tm3886) IV.
Alternate IDs:
Notes: Superficially wild-type. Deletion site verified by PCR. Reference: Meier B, et al. Genome Res. 2014 Oct;24(10):1624-36.
Proper citation: RRID:WB-STRAIN:WBStrain00051050 Copy
http://www.wormbase.org/db/get?name=WBStrain00051058
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00018908(fncm-1)
Genomic Alteration: WBGene00018908(fncm-1)
Availability: unknown
References:
Synonyms: fncm-1(tm3148) I.
Alternate IDs:
Notes: Superficially wild-type. Deletion site verified by PCR. Reference: Volkova NV, et al. Nat. Commun. 2020 May 1;11(1):2169.
Proper citation: RRID:WB-STRAIN:WBStrain00051058 Copy
http://www.wormbase.org/db/get?name=WBStrain00051057
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00020935(fnci-1)
Genomic Alteration: WBGene00020935(fnci-1)
Availability: unknown
References:
Synonyms: fnci-1(tm3081) I.
Alternate IDs:
Notes: Superficially wild-type. Deletion site verified by PCR. Reference: Volkova NV, et al. Nat. Commun. 2020 May 1;11(1):2169.
Proper citation: RRID:WB-STRAIN:WBStrain00051057 Copy
http://www.wormbase.org/db/get?name=WBStrain00051041
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00017546(rpa-1)|WBGene00019079(rpa-4)|WBGene00019767(rpa-2)
Genomic Alteration: WBGene00017546(rpa-1), WBGene00019079(rpa-4), WBGene00019767(rpa-2)
Availability: unknown
References:
Synonyms: rpa-4(iow128[Myc::rpa-4]) rpa-2(iow49[3xFLAG::rpa-2]) I; rpa-1(iow92[OLLAS::rpa-1]) II.
Alternate IDs:
Notes: CRISPR/Cas9 engineering used to insert N-terminal Myc tag into the endogenous rpa-4 locus, N-terminal 3xFLAG tag into the endogenous rpa-2 locus, and N-terminal OLLAS tag into the endogenous rpa-1 locus. SSM559 was generated by crossing rpa-2(iow49) with rpa-1(iow92), followed by CRISPR insertion of the Myc tag into rpa-4. Reference: Hefel et al., Nucleic Acids Res. 2021 Jan 21;gkaa1293. doi: 10.1093/nar/gkaa1293.|"Made_by: Adam Hefel"
Proper citation: RRID:WB-STRAIN:WBStrain00051041 Copy
http://www.wormbase.org/db/get?name=WBStrain00051040
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00017546(rpa-1)
Genomic Alteration: WBGene00017546(rpa-1)
Availability: unknown
References:
Synonyms: rpa-1(iow92[OLLAS::rpa-1]) II.
Alternate IDs:
Notes: Made_by: Adam Hefel|"N-terminal OLLAS tag inserted into the endogenous rpa-1 locus using Crispr/Cas9. Generated in N2 background. Reference: Hefel et al., Nucleic Acids Res. 2021 Jan 21;gkaa1293. doi: 10.1093/nar/gkaa1293."
Proper citation: RRID:WB-STRAIN:WBStrain00051040 Copy
http://www.wormbase.org/db/get?name=WBStrain00051045
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004393(rnt-1)
Genomic Alteration: WBGene00004393(rnt-1)
Availability: unknown
References:
Synonyms: rnt-1(he305[rnt-1::eGFP::3xflag::loxP]) I.
Alternate IDs:
Notes: eGFP and 3xFlag tags inserted into endogenous rnt-1 locus. Superficially wild-type. Reference: Horst SEM, et al. Development 2019 Nov 18;146(22):dev180034.|"Made_by: Van den Heuvel lab"
Proper citation: RRID:WB-STRAIN:WBStrain00051045 Copy
http://www.wormbase.org/db/get?name=WBStrain00051044
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: hrtSi57 II; unc-119(ed3) III.
Alternate IDs:
Notes: hrtSi57 [gcy-36p::gcy-35::mKate2 + unc-119(+)] II. hrtSi57 inserted into ttTi5605. mKate2 inserted into gcy-35 after S671 to generate a functional expression construct (Gross et al., 2014). Reference: Harterink M, et al. J Cell Sci. 2018 Oct 22;131(20):jcs223107. PMID: 30254025.
Proper citation: RRID:WB-STRAIN:WBStrain00051044 Copy
http://www.wormbase.org/db/get?name=WBStrain00051043
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
References:
Synonyms: hrtSi27 II; unc-119(ed3) III.
Alternate IDs:
Notes: hrtSi27 [des-2p::CRE + unc-119(+)] II. hrtSi27 inserted into ttTi5605. CRE expression in PVD and FLP. Reference: Harterink M, et al. J Cell Sci. 2018 Oct 22;131(20):jcs223107. PMID: 30254025.
Proper citation: RRID:WB-STRAIN:WBStrain00051043 Copy
http://www.wormbase.org/db/get?name=WBStrain00051042
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001072(dpy-10)|WBGene00017546(rpa-1)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00017546(rpa-1)
Availability: unknown
References:
Synonyms: rpa-1(iow117)/mIn1[mIs14 dpy-10(e128)] II.
Alternate IDs:
Notes: Crispr/Cas9-engineered indel in the 5 region of rpa-1. Larval-lethal mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP iow117 homozygotes (larval lethal). Pick wild-type dim GFP and check for correct segregation of progeny to maintain. iow117 was generated in mre-11::GFP background and outcrossed to N2. Reference: Hefel et al., Nucleic Acids Res. 2021 Jan 21;gkaa1293. doi: 10.1093/nar/gkaa1293.|"Made_by: Adam Hefel"
Proper citation: RRID:WB-STRAIN:WBStrain00051042 Copy
http://www.wormbase.org/db/get?name=WBStrain00051048
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00013241(ung-1)
Genomic Alteration: WBGene00013241(ung-1)
Availability: unknown
References:
Synonyms: ung-1(tm2862) III.
Alternate IDs:
Notes: Superficially wild-type. Deletion site verified by PCR. Reference: Meier B, et al. Genome Res. 2014 Oct;24(10):1624-36.
Proper citation: RRID:WB-STRAIN:WBStrain00051048 Copy
http://www.wormbase.org/db/get?name=WBStrain00051139
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003062(lpd-6)|WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003062(lpd-6), WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
References:
Synonyms: lpd-6(gk5418[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ I.
Alternate IDs:
Notes: Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 3234 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: TAAATCCTCCATCACGATCTCCCGATCTTC. Right flanking sequence: CTGACGACAAGTTTTTTACCGCGATTTCCG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051139 Copy
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