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http://www.salk.edu/science/core-facilities/media-preparation-core/
Core facility that provides media, plates, and commonly used buffers for molecular biology applications and culturing mammalian cells, C. elegans, yeast, and bacteria. The Media Preparation Core Facility is equipped with 3 automatic plate pourers supported by 2 programmable media kettles capable of producing up to 9 liters of agar or nutrient media per run under gentle and reproducible conditions. Plates are available in 6 cm or 10 cm formats.
Proper citation: Salk Institute Media Preparation Core Facility (MPRP) (RRID:SCR_014844) Copy
http://www.sbpdiscovery.org/technology/sr/Pages/LaJolla_StemCells.aspx
THIS RESOURCE IS NO LONGER IN SERVICE, documented August 23, 2016. The former functions of this facility are split into two separate operations. The first is the generation and characterization of induced Pluripotent Stem Cells (iPSCs) is now being performed on a collaborative basis for both internal and external investigators with the Snyder lab. The second is a shared laboratory dedicated to the culture and analysis of stem cells that is available to SBP investigators.
Proper citation: Sanford Burnham Prebys Medical Discovery Institute Stem Cell Core (RRID:SCR_014856) Copy
http://www.sbpdiscovery.org/technology/sr/Pages/LaJolla_TumorAnalysis.aspx
Facility that provides analysis of animal models of human cancer and other diseases. It provides investigators access to a wide variety of human cancer cell lines for xenograft studies, some primary human xenograft models and additional transgenic mouse solid tumors and leukemia models. The core also offers serial passaging of tumors and derivation of 2D and 3D cultures from xenograft tumors, including patient-derived xenograft (PDX) models. These short-term cell cultures established from PDXs enable in vitro analysis including high throughput screening with compounds or RNAi for functional characterization.
Proper citation: Sanford Burnham Prebys Medical Discovery Institute Tumor Analysis (RRID:SCR_014858) Copy
Center for resources to facilitate basic and preclinical applications of the transgenic Huntington's disease (HD) monkey model to advance scientific knowledge and the discovery of a cure for HD. Their resources include longitudinal biomaterials and MRI data, postmortem biomaterials, and research proposal assistance.
Proper citation: Transgenic Huntington's Disease Monkey Resources (RRID:SCR_014560) Copy
Center that imports, archives, maintains, and distributes mutant mouse alleles as live mice, frozen germplasm, stem cells, and molecular vectors for use in biomedical research. The MMRRC Davis receives transgenics, knockouts, and other kinds of mutant mouse lines at no cost to the donor, and after re-derivation and cryopreservation, distributes breeding stock, germplasm, cells, or tissues of genetically-defined and pathogen-free mice for a small fee to requesting investigators.
Proper citation: University of California at Davis Mutant Mouse Resource and Research Center (RRID:SCR_016448) Copy
http://www.mmrrc.missouri.edu/
Center that supplies mice and conducts research projects focused on the role of mice as animal models. Some of these projects include refinement of models to ensure study reproducibility, as well as development and improvement of economical methods for cryopreservation of mouse strains.
Proper citation: Mutant Mouse Resource and Research Center - University of Missouri (RRID:SCR_016447) Copy
http://www.vet.upenn.edu/research/core-resources-facilities/referral-center-for-animal-models
Center that aims to discover, characterize, maintain breeding colonies, and make available dog and cat models with hereditary diseases homologous to those found in human patients that can be used to translate preclinical trials from kennel to clinic. The animal models represent true orthologs of their respective human disease, involving defects in homologous genes resulting in similar molecular, biochemical, pathological, and clinical phenotype as in human patients.
Proper citation: Referral Center for Animal Models of Human Genetic Diseases (RRID:SCR_016453) Copy
Commercial provider of antibodies, antigens and recombinant proteins from Braintree, Massachusetts . Biotechnology company which provides custom oligonucleotides synthesis service.
Proper citation: kbDNA Inc. (RRID:SCR_016570) Copy
One of nine Councils within United Kingdom Research and Innovation, an organisation that brings together the UK’s Research Councils, Innovate UK and Research England. Works in partnership with universities, research organisations, businesses, charities, and government to create the environment for research and innovation.
Proper citation: Science and Technology Facilities Council (RRID:SCR_016713) Copy
THIS RESOURCE IS NO LONGER IN SERVICE. Documented on November 14,2023.Automated preparation system that isolates and prepares single cell templates for analysis. It can analyze mRNA and DNA sequencing, epigenetics or miRNA expression. It can extract, reverse transcribe, amplify, and analyze gene expression.
Proper citation: Fluidigm: C1 Single-Cell Automated Preparation System (RRID:SCR_018578) Copy
Sliding microtome from Leica Biosystems with low maintenance cross roller bearing slideways designed to easily section either paraffin embedded or fixed, frozen specimens. Features knife holder for both standard knives and disposable blades. Please note: this product is discontinued and no longer available for sale.
Proper citation: Leica: SM2000 R Sliding microtome (RRID:SCR_018456) Copy
https://www.marshallscientific.com/Fisher-Scientific-PowerGen-125-Homogenizer-p/fs-pg125.htm
Homogenizer with speeds from 8,000 to 30,000 RPM for volumes of up to 100ml. Homogenizer for production of dispersions and emulsions, plant tissues, human tissues, animal tissues, resins, and pigments.
Proper citation: Thermo Fisher: n125 Homogenizer (RRID:SCR_018576) Copy
https://www.marshallscientific.com/Fisher-Scientific-Isotemp-280A-Vacuum-Oven-p/fs-280a.htm
Fisher Scientific Isotemp 280A is a Vacuum Oven offers a temperature range from ambient up to 200C with internal dimensions of 11.125"D x 9.75"W x 9.75"H. Front-mounted power indicator and dial thermometer with top-mounted analog vacuum gauge. The 280A's hydraulic thermostat controls temperature. Large glass panel front door and stainless steel chamber.
Proper citation: Thermo Fisher: Isotemp 280A Vacuum Oven (RRID:SCR_018575) Copy
https://www.chiropracticoutfitters.com/9W-Pilot-Laser-p/pilot-diode-laser.htm
Diode laser, 810 nm wavelength with manufacturer from CAO group, USA. Commodity code: 002-00102. Used for rehabilitation procedures to promote healing, reduce pain and inflammation.
Proper citation: Pilot: 9W Pilot Diode Laser (RRID:SCR_018458) Copy
https://www.gilson.com/verity-271-hplc-system.html
HPLC liquid handler with midsize bed capacity and fraction collector. It has maximum flow rates up to 200 mL per min, injection loop volumes of 2 uL to 25 mL, and max sample mass of 15 grams per injection.
Proper citation: Gilson: GX-271 HPLC System (RRID:SCR_018579) Copy
Fully automated Leica VT1200 S vibrating blade microtome designed for sectioning for cutting fresh and fixed tissue in neuroscience by Leica Biosystems.
Proper citation: Leica: VT1200S vibratome (RRID:SCR_018453) Copy
https://precisionary.com/compresstome-vf-300-vibrating-microtome/
Vibrating microtome for cutting tissue slices, similar to cryostats and vibrating microtomes. Fully automated for sectioning fixed and live tissues, with down to 1 um precision. Can cut tissue slice thicknesses ranging from 3 um to 2000 um, at 1 um intervals.
Proper citation: Precisionary: Compresstome VF-300 (RRID:SCR_018452) Copy
https://pubmed.ncbi.nlm.nih.gov/25172804/
Probe uses semi permeable membrane with pore size of 30 kDa which enables small hydrophilic compounds from brain extracellular space to diffuse passively into perfusate. Used to measure extracellular ACh level and glucose metabolites directly without purification.
Proper citation: Custom made 30 kDa microdialysis probe (RRID:SCR_018571) Copy
http://www.limef.com/Downloads/752-752M%20manual.pdf
Vibrating microtome manufactured by Campden Instruments. 752 Vibroslice tissue cutter for manual tissue transport.
Proper citation: Campden Instruments: Vibroslice vibrating microtome (RRID:SCR_018460) Copy
EImaging scanner that images fluorescent and radioisotopic samples. It can be used for protein electrophoresis, genomics array analysis, fluorescence, radioisotope detection, and chemiluminescence detection by direct detection method.
Proper citation: Fujifilm: FLA-5100 Scanner (RRID:SCR_018581) Copy
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