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  • Organism:xenopus laevis (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pFIN-XOPS-tdTOM-MOPS-GFP
 
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RRID:Addgene_44347 XOPS-tdTOM Xenopus laevis Ampicillin PMID:20517486 Backbone Marker:LJ Chang; Backbone Size:8760; Vector Backbone:pTY; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:15:14 0
GFP-Intersectin Full Length
 
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RRID:Addgene_47407 Intersectin Xenopus laevis Kanamycin Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:42 0
M20 siamois-luciferase p01234
 
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RRID:Addgene_17157 siamois promoter Xenopus laevis Ampicillin PMID:9308964 Plasmid History: Constructed by David Kimelman, 6/6/96 XVII p. 98. Notes for Use: The -3.0kb-siamois promoter was directionally cloned into the KpnI and BglII sites of the luciferase reporter vector pGL3B (Promega) upstream of the luciferase ATG. The -0.8kb promoter construct was generated by digesting the -3.0kb/pGL3B construct with KpnI and at an internal EcoRI site to remove 2.2 kb of intervening sequence, filling in with DNA polymerase I large fragment and religating. The Kpn site was lost but the R1 site was regenerated in the ligation. The size of the insert is approximate. Citation: Brannon et al., Gene and Development 11:2359-2370, 1997. Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:10:17 0
3.8NBetaT-CAT
 
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1+ mentions
RRID:Addgene_17146 Beta-tubulin promoter Xenopus laevis Ampicillin Constructed Rebecca Beach and Paul A. Kreig. 3.8Kb fragment of the Xenopus neural Beta-tubulin promoter inserted (in several cloning steps) into the CAT expression vector pBLCAT3. The 5' end of the neural Beta-tubulin sequence is defined by the natural Xba1 site that occurs in the genomic DNA. The 3' end of the insert is produced by PCR primer located 8bp upstream of hte initiation ATP and 120bp downstream of the transcription start site. The neural Beta-tubulin sequences may be excised as a Hind3 fragment. Backbone Marker:ATCC; Backbone Size:4300; Vector Backbone:pBLCAT3; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:10:16 1
M10 NCAM Promoter
 
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RRID:Addgene_17147 NCAM promoter Xenopus laevis Ampicillin Plasmid History: Constructed Paul A. Kreig, July. Note for Use: This construction contains a 1.3 Kb Dra I fragment of the NCAM promoter and should be okay for general neural expression. The fragment is cloned into the Sma I site of Bluescript KS+. The NCAM promoter does not contain a TATA box and transcription starts at numerous sites over about 350 bases. All of the potential start sites are contained in this 1.3 Kb fragment, which ends in the 5' UTR of the NCAM gene. The Dra I fragment starts at the very beginning of the GenBank sequence (starting with aaa) and ends at base 1358 of the GenBank sequence. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:16 0
M11 Cardiac Actin Promoter pCarA
 
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1+ mentions
RRID:Addgene_17148 cardiac actin promoter Xenopus laevis Ampicillin Plasmid History: Constructed by Enrique Amaya. Note for Use: Constructed by cloning the 3270bp KpnI-SalI fragment from DNA#254 (from Tim Mohun) into KpnI-SalI digested pCSKA. Backbone Size:4000; Vector Backbone:pCSKA; Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:18 1
M12 pCarGFP2
 
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RRID:Addgene_17149 cardiac actin promoter Xenopus laevis Ampicillin Plasmid History: Constructed by Enrique Amaya. Note for Use: Generated by inserting the 1Kb HindIII-NotI fragment from pCSGFP2 into HindIII-NotI digested pCarG. This GFP constuct has been modified by Jim Haseloff and Jonathan Pines to be brighter and more soluble that wild-type GFP. Note: Uniques sites are underlined in Author's Map. Backbone Size:4000; Vector Backbone:pCSKA; Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:18 0
pGEX-4T-1-Xlrbpa-dsRBD(+6)
 
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RRID:Addgene_171548 Xlrbpa Xenopus laevis Ampicillin PMID:33751023 Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin a six amino acid_TRLTEG_insertion in the L1 region 2026-08-15 01:10:17 0
pGEX-4T-1-Xlrbpa
 
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RRID:Addgene_171547 Xlrbpa Xenopus laevis Ampicillin PMID:33751023 Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:17 0
-226bp goosecoid luciferase
 
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RRID:Addgene_17158 goosecoid promoter Xenopus laevis Ampicillin PMID:8543150 Generated by PCR using the 3' primer (nucleotides - 5 to + 12 relative to the transcription start site) and 5' primer to -226. The 3' primer contained a HindIII restriction site at its 5' terminus and the 5' primer contained a BamHI restriction site at its 5' terminus for directional cloning into pOLuc. Backbone Size:0; Vector Backbone:pOLuc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:10:17 0
-1.5 kb goosecoid luciferase
 
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RRID:Addgene_17159 goosecoid promoter Xenopus laevis Ampicillin PMID:8543150 The - 1500gsc/Luc reporter was constructed from a blunt-ended EcoRV-PstI 1.5kb Xenopus gsc gene fragment carrying 3 bp of exon 1 and 5'-promoter sequences inserted into the SmaI site of the promoterless luciferase vector pOLuc (de Wet et al. 1987). Backbone Size:0; Vector Backbone:pOLuc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:10:17 0
M35 Dpr yeast clone
 
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RRID:Addgene_17191 XDpr Xenopus laevis Ampicillin PMID:11970895 Description: Dpr yeast clone in original prey vector. 1.6kb EcoR1 fragment corresponds to C-terminal 1.2 kb of Xenopus Dpr and 3' UTR in pGAD vector. Reference: Cheyette, B. et al., 2002, Developmental Cell 2: 449-461. Backbone Marker:Clontech; Backbone Size:8000; Vector Backbone:pGAD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:20 0
M36 Dpr C-terminal GST
 
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RRID:Addgene_17192 XDpr Xenopus laevis Ampicillin PMID:11970895 Description: Coding sequence of original XDpr prey clone (M35) cloned into pGEX vector as a 1.2 kb EcoR1-Xba1 fragment (Xba site is DAM methylated). Reference: Cheyette, B. et al., 2002, Developmental Cell 2: 449-461. Backbone Size:0; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:20 0
M61 pPET28c-Xenopus beta catenin domains R10C
 
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RRID:Addgene_17202 beta catenin Xenopus laevis Kanamycin Description: This is an amino terminal HIS tag, then beta catenin domains R10C, subcloned into BamH1 site. Reference: Made by Ken-Ichi Takemaru. Contains ARM domains 8-10 and C-terminal end of protein (starts with aa519) Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin domains R10C 2026-08-15 01:10:21 0
pCS2+-wbp2nlT45A
 
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RRID:Addgene_172464 wbp2nlT45A Xenopus laevis Ampicillin PMID:28663133 we cloned this plasmid Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin changed Threonine at aa45 to Alanine 2026-08-15 01:10:24 0
pCS2-xFAM3b.L-HA
 
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RRID:Addgene_172852 xFAM3B.L Xenopus laevis Ampicillin PMID:30209221 Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:28 0
XE247 Xenopus CK1 epsilon K to R CS2+
 
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RRID:Addgene_16725 casein kinase 1, epsilon Xenopus laevis Ampicillin PMID:15539487 Description: From John Graff, in Peters et al 1999. Notes for Use: Select clones with amp. Reference: used in Waxman JS, Hocking AM, Stoick CL, Moon RT. Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Development. 2004 Dec;131(23):5909-21. Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin K38R 2026-08-15 01:09:42 0
XE90 pCS2P+ XCtBP
 
Resource Report
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RRID:Addgene_16704 XCtBP Xenopus laevis Ampicillin Plasmid History: Constructed by Jeff Brown. Notes for Use: The ~2 kb dropout of the Sal I digest of XTcf-3 interacting clone 2-1 (in pGBT9) was ligated into the XhoI site of pCS2P+. For CS2+MT XCtBP, the Sal I dropout was blunted with Klenow and cloned into the StuI site of pCS2+MT. Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:41 0
XE51 XPKC-alpha
 
Resource Report
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RRID:Addgene_16746 XPKC-Alpha Xenopus laevis Ampicillin PMID:1547501 SmaI linearize, SP6 for sense RNA. Notes for Use: Ref: used in Otte and Moon, Cell 68:1021-1029, 1992. Backbone Marker:Promega; Backbone Size:2869; Vector Backbone:pGem2; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:44 0
XE105 XPKC alpha-myc-CS2+
 
Resource Report
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RRID:Addgene_16745 XPKC-alpha-myc Xenopus laevis Ampicillin PMID:10395542 Insert: Xenopus PKC alpha with a c-myc epitope at carboxy terminus. For transcription cut with Not 1 and use SP6. Notes for Use: Ref: Sheldahl et al., Current Biology 9:695-698, 1999. Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:44 0

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