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  • Organism:drosophila melanogaster (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pUASTattB_NSlmb-vhhGFP4
 
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RRID:Addgene_35577 NSlmb-vhhGFP4 Drosophila melanogaster Ampicillin PMID:22157958 Backbone Size:8500; Vector Backbone:pUASattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:02 0
pUAST_NSlmb-vhhGFP4
 
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RRID:Addgene_35575 NSlmb-vhhGFP4 Drosophila melanogaster Ampicillin PMID:22157958 Backbone Size:8800; Vector Backbone:pUAS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:06 0
pUASTattB_NSnoFbox-vhhGFP4
 
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RRID:Addgene_35578 NSnoFbox-vhhGFP4 Drosophila melanogaster Ampicillin PMID:22157958 Backbone Size:8500; Vector Backbone:pUASattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin F box domain of slmb deleted. 2026-08-15 01:14:02 0
pENTR-E2F1-PIP3A
 
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RRID:Addgene_37142 E2F1-P!P3A Drosophila melanogaster Kanamycin PMID:19081076 Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin I153, Y156, Y157 to Alanine 2026-08-15 01:14:13 0
pCaShs-hopscotch
 
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RRID:Addgene_37299 hopscotch Drosophila melanogaster Ampicillin PMID:7796812 Perrimon Lab plasmid #99 Backbone Size:8900; Vector Backbone:pCaSpeR-hs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:14 0
ovoD1 D1B2H (7.2 kb)
 
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RRID:Addgene_37282 ovoD1 genomic DNA fragment Drosophila melanogaster Ampicillin PMID:8306893 Perrimon Lab plasmid #25 Please see associated publication for cloning details Backbone Size:7815; Vector Backbone:pCaSpeR 2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:14 0
orthodenticle
 
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RRID:Addgene_37285 orthodenticle Drosophila melanogaster Ampicillin PMID:1979296 Perrimon Lab plasmid #46 Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBlueScript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:14 0
10XSTAT92E–luciferase
 
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RRID:Addgene_37393 SOCS36E enhancer fragment Drosophila melanogaster Ampicillin PMID:16055650 Perrimon Lab plasmid #446 A 441-bp genomic fragment in the enhancer of SOCS36E containing two potential STAT92E-binding sites was amplified by PCR, using five different sets of oligos: (1) CTGCAGGAACCACTCAGAGTGCCTGCGTGT (PstI), GAATTCATACAAAACTGTCTTAGGTGTTTA (EcoRI); (2) CTGCAGGAACCACTCAGAGTGCCTGCGTGT (PstI), CTGCAGATACAAAACTGTCTTAGGTGTTTA (PstI); (3) GAATTCGAACCACTCAGAGTGCCTGCGTGT (EcoRI), GAATTCATACAAAACTGTCTTAGGTGTTTA (EcoRI); (4) AGATCTGAACCACTCAGAGTGCCTGCGTGT (BglII), AGATCTATACAAAACTGTCTTAGGTGTTTA (BglII); (5) GCGGCCGCGAACCACTCAGAGTGCCTGCGTGT (NotI), GCGGCCGCATACAAAACTGTCTTAGGTGTTTA (NotI). Each amplified genomic fragment containing different restriction enzyme sites was sequentially subcloned into pUAST. The genomic fragment amplified using the first set of oligos was subcloned into the PstI/EcoRI sites of pUAST to generate 2XSTAT92E. The genomic fragment amplified using the second set of oligos was subcloned into the PstI site of 2XSTAT92E to generate 4XSTAT92E. The genomic fragment amplified using the third set of oligos was subcloned into the EcoRI site of 4XSTAT92E to generate 6XSTAT92E. The genomic fragment amplified using the fourth set of oligos was subcloned into the BglII site of 6XSTAT92E to generate 8XSTAT92E. Next, the hsp70 minimal promoter element was amplified from pUAST by PCR using oligos GCGGCCGCAGCGGAGACTCTAGCGAGCG (NotI) and CTCGAGAATTCCCTATTCAGAGTTCT (XhoI). This hsp70 minimal promoter was subcloned into the NotI/XhoI sites of 8XSTAT92E to generate 8XSTAT92E–hsp70. Again, the genomic fragment amplified using the fifth set of oligos was subcloned into the NotI site of 8XSTAT92E–hsp70 vector to generate 10XSTAT92E–hsp70. Finally, an XhoI/XbaI fragment containing the firefly luciferase gene from the pGL3–luciferase vector (Promega) was subcloned into the XhoI/XbaI sites of 10XSTAT92E–hsp70 to generate 10XSTAT92E–luciferase. Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression, Luciferase, JAK/STAT reporter construct; Bacterial Resistance:Ampicillin 2026-08-15 01:14:14 2
ovoD1 D1B2NR (18 kb)
 
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RRID:Addgene_37280 ovoD1 genomic DNA fragment Drosophila melanogaster Ampicillin PMID:8306893 Perrimon Lab plasmid #23 Please see associated publication for cloning details Backbone Size:7815; Vector Backbone:pCaSpeR 2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:14 0
pUAS-hopscotch
 
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RRID:Addgene_37301 hopscotch Drosophila melanogaster Ampicillin PMID:7796812 Perrimon Lab plasmid #100 Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:14 0
pCaShs-Tum
 
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RRID:Addgene_37303 hopTum-1 Drosophila melanogaster Ampicillin PMID:7796812 Perrimon Lab plasmid #101 The pCaShs-Tum construct was generated by the replacement of a 220 bp SacII-BstEII fragment from pCaShs-hop (Addgene plasmid# 37299) with the same fragment of PCR-generated DNA from hopTum-1 hemizygous flies. This mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341. Backbone Marker:Addgene plasmid# 37299; Vector Backbone:pCaShs-hopscotch; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Tul-l mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341 2026-08-15 01:14:14 0
pUAS-Tum
 
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RRID:Addgene_37304 hopTum-1 Drosophila melanogaster Ampicillin PMID:7796812 Perrimon Lab plasmid #102 The pUAS-Tum was made by the insertion of the NotI-XbaI fragment from pCaShs-Tum (Addgene plasmid# 37303) into pUAST. Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin Tul-l mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341 2026-08-15 01:14:14 0
pCaSpeR-4 porcupine (genomic DNA)
 
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1+ mentions
RRID:Addgene_37376 porcupine Drosophila melanogaster Ampicillin PMID:8985181 Perrimon Lab plasmid #123 The EcoRI-XbaI genomic DNA covering the porc locus was cloned in pCaspeR4 to generate a genomic porc rescue construct. Backbone Size:7855; Vector Backbone:pCaSpeR-4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:14 1
porcupine
 
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RRID:Addgene_37377 porcupine Drosophila melanogaster Ampicillin PMID:8985181 Perrimon Lab plasmid #124 Vector Backbone:pNB40; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:14:14 0
tout velou (ttv)
 
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RRID:Addgene_37401 tout-velu Drosophila melanogaster Ampicillin PMID:9665133 Perrimon Lab plasmid #179 Vector Backbone:pNB40; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:14:14 0
pCFJ1209
 
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RRID:Addgene_44491 Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR] Drosophila melanogaster Ampicillin PMID:24820376 Backbone Marker:Invitrogen; Backbone Size:2445; Vector Backbone:pDESTR4-R3; Vector Types:Gateway expression clone; Bacterial Resistance:Ampicillin 2026-08-15 01:15:15 0
pCFJ1200
 
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RRID:Addgene_44485 Minimal Mos1, PuroR, peel-1, MCS Drosophila melanogaster Ampicillin PMID:24820376 Backbone Marker:Invitrogen; Backbone Size:2305; Vector Backbone:pDESTR4-R3; Vector Types:Multiple cloning site vector; Bacterial Resistance:Ampicillin 2026-08-15 01:15:15 0
pCFJ1202
 
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1+ mentions
RRID:Addgene_44484 Minimal Mos1, NeoR, peel-1, MCS Drosophila melanogaster Ampicillin PMID:24820376 Backbone Marker:Invitrogen; Backbone Size:2305; Vector Backbone:pDESTR4-R3; Vector Types:Multiple cloning site vector; Bacterial Resistance:Ampicillin 2026-08-15 01:15:16 1
pCFJ1272
 
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RRID:Addgene_44486 Minimal Mos1, MCS Drosophila melanogaster Ampicillin PMID:24820376 Backbone Marker:Invitrogen; Backbone Size:2305; Vector Backbone:pDESTR4-R3; Vector Types:Multiple cloning site vector; Bacterial Resistance:Ampicillin 2026-08-15 01:15:15 0
pCFJ914
 
Resource Report
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1+ mentions
RRID:Addgene_44489 Minimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR] Drosophila melanogaster Ampicillin PMID:24820376 Backbone Marker:Invitrogen; Backbone Size:2445; Vector Backbone:pDESTR4-R3; Vector Types:Gateway expression clone; Bacterial Resistance:Ampicillin 2026-08-15 01:15:15 2

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