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Species: Arabidopsis thaliana
Genetic Insert: UBQ10 promoter
Vector Backbone Description: Backbone Marker:Mark Curtis at ETH Zurich (Brand et al., Plant Physiology 2006); Vector Backbone:pLB12; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Comments: The vector pLB12 contains both an activator unit and a b-glucuronidase (GUS) reporter within a responder unit.
Proper citation: RRID:Addgene_53449 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter, RbcS3B (AT5g38410, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50260 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4672; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Proper citation: RRID:Addgene_103816 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4725; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Proper citation: RRID:Addgene_103815 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-iRFP713
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3945; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: Switched iRFP713 (PCR on Addgene #31857) for mCherry in pCRY2PHR-mCherry-N1 (Addgene #26866) using AgeI & NotI (AgeI site destroyed)
Proper citation: RRID:Addgene_103818 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-EYFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3945; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: Switched EYFP for mCherry in pCRY2PHR-mCherry-N1 (Addgene #26866)
Proper citation: RRID:Addgene_103817 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN-LacI
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3932; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: EGFP was cut out from the backbone (NheI/BamHI cuts); relevance of mutations unknown
Proper citation: RRID:Addgene_103814 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-iRFP713-VP16
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3945; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: aa412-482 of UL48 (transactivation domain); mCherry was cut out from the backbone (XhoI/NotI cuts)
Proper citation: RRID:Addgene_103823 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-mCherry-VP16
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3945; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: aa412-482 of UL48 (transactivation domain); mCherry was cut out from the backbone (XhoI/NotI cuts) and reinserted with the insert
Proper citation: RRID:Addgene_103821 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-EYFP-NLS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3945; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: Switched EYFP for mCherry in pCRY2PHR-mCherry-N1 (Addgene #26866) and inserted NLS by PCR
Proper citation: RRID:Addgene_103820 Copy
Species: Arabidopsis thaliana
Genetic Insert: thalianol synthase 1
Vector Backbone Description: Backbone Marker:Sergey V. Shestakov and Anne Karradt and Wolfgang Lockau; Vector Backbone:pVZ-spec; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:29281679
Comments: second gene: squalene epoxidase (SQE1)
third gene: coaR repressor protein from Synechoccystis sp. PCC6803
growth temperature (37C) referes to E. coli ; in Synechoccystis sp. PCC6803: 30 C
Proper citation: RRID:Addgene_103859 Copy
Species: Arabidopsis thaliana
Genetic Insert: lupeol synthase 1
Vector Backbone Description: Backbone Marker:Sergey V. Shestakov and Anne Karradt and Wolfgang Lockau; Vector Backbone:pVZ-spec; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:29281679
Comments: second gene: squalene epoxidase (SQE1)
third gene: coaR repressor protein from Synechoccystis sp. PCC6803
growth temperature (37C) referes to E. coli ; in Synechoccystis sp. PCC6803: 30 C
Proper citation: RRID:Addgene_103857 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2(neutral2-6)-mCherry
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28916751
Proper citation: RRID:Addgene_104181 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2low-mCherry
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28916751
Proper citation: RRID:Addgene_104065 Copy
Species: Arabidopsis thaliana
Genetic Insert: GAI-tagBFP-Sp dCas9-ABI
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:PB; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, PiggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27776111
Proper citation: RRID:Addgene_106895 Copy
Species: Arabidopsis thaliana
Genetic Insert: CPK30
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28489820
Comments: Addgene NGS results show that this plasmid contains amino acids 1-322 of the CPK30 translation
Proper citation: RRID:Addgene_107429 Copy
Species: Arabidopsis thaliana
Genetic Insert: CPK10
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28489820
Proper citation: RRID:Addgene_107428 Copy
Species: Arabidopsis thaliana
Genetic Insert: sgRNA-transRNA transcription by U6 promoter
Vector Backbone Description: Backbone Marker:Lampropoulos et al., 2013; Vector Backbone:pE000; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30083222
Proper citation: RRID:Addgene_104441 Copy
Species: Arabidopsis thaliana
Genetic Insert: sgRNA-transRNA transcription by U6 promoter
Vector Backbone Description: Backbone Marker:Lampropoulos et al., 2013; Vector Backbone:pD000; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30083222
Proper citation: RRID:Addgene_104440 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cry2-mCherry-PKA W196R/F327A
Vector Backbone Description: Backbone Marker:Chandra Tucker; Backbone Size:6219; Vector Backbone:pmCherryCry2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29104065
Proper citation: RRID:Addgene_104547 Copy
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