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Species: Homo sapiens
Genetic Insert: erythropoietin(EPO)
Vector Backbone Description: Vector Backbone:pLV-EF1alpha; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28602759
Proper citation: RRID:Addgene_139057 Copy
Genetic Insert: 3XHA-EGFP-PEX26
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32417403
Proper citation: RRID:Addgene_139054 Copy
Species: Mus musculus
Genetic Insert: PrP
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pCB6+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11742063
Comments: Full length mouse PrP with 3F4 epitag was cloned into HindIII and BamHI sites of mammalian cell expression vector pCB6+.
Proper citation: RRID:Addgene_13917 Copy
Species: Homo sapiens
Genetic Insert: MFN2
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22748923
Proper citation: RRID:Addgene_139192 Copy
Species: Staphylococcus pyogenes
Genetic Insert: dSpCas9-VPR
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32130891
Proper citation: RRID:Addgene_139090 Copy
Species: Staphylococcus pyogenes
Genetic Insert: KRAB-dxCas9-NG
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32130891
Proper citation: RRID:Addgene_139097 Copy
Species: Homo sapiens
Genetic Insert: iRFP:MTOR(2021-2113):[GGSA]2:GOLGB1(3097-3226)
Vector Backbone Description: Vector Backbone:piRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139313 Copy
Species: Mus musculus
Genetic Insert: mCherry:Mus musculus Prkd1(138-343)
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139314 Copy
Genetic Insert: hChR2(H134R)-P2A-EYFP
Vector Backbone Description: Vector Backbone:pAAV-EF1a-DIO; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32073399
Proper citation: RRID:Addgene_139283 Copy
Species: Mus musculus
Genetic Insert: Wnt-4
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10862751
Comments: Mouse Wnt-4 full-length ORF:
5’BsaI-primer: GGTCTCCATG AGCCCCCGTT CGTGC
3’EcoRI-primer: CTGAATTCAC CGGCACGTGT GCATCTCC.
Proper citation: RRID:Addgene_13937 Copy
Genetic Insert: linker with pelB-leader, cloning site, flag-tag and p3
Vector Backbone Description: Vector Backbone:fADL-1e; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32872428
Proper citation: RRID:Addgene_139441 Copy
Species: Synthetic
Genetic Insert: no sgRNA inserted; resistance gene: neoR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52963); Vector Backbone:LentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Comments: Generated by replacing PuroR gene with NeoR gene in LentiGuide-Puro from Feng Zhang (Addgene plasmid # 52963).
Proper citation: RRID:Addgene_139449 Copy
Species: Homo sapiens
Genetic Insert: FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform)
Vector Backbone Description: Backbone Marker:PMID: 24048477; Vector Backbone:RRL.sin.cPPT.CMV/IRES-puro.WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139447 Copy
Species: Synthetic
Genetic Insert: Non-targeting sgRNA inserted; resistance gene: puroR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52963); Vector Backbone:LentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139456 Copy
Genetic Insert: DTECT ligation product
Vector Backbone Description: Backbone Size:3500; Vector Backbone:TOPO; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139333 Copy
Species: Synthetic
Genetic Insert: Non-targeting sgRNA inserted; resistance gene: puroR
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52963); Vector Backbone:LentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139455 Copy
Species: Synthetic
Genetic Insert: Curlcake4 with T7 promoter
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501426
Proper citation: RRID:Addgene_139343 Copy
Species: Synthetic
Genetic Insert: Curlcake2 with T7 promoter
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501426
Proper citation: RRID:Addgene_139341 Copy
Species: Homo sapiens
Genetic Insert: TYK2 cDNA with 267nt of 5' UTR
Vector Backbone Description: Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31961910
Proper citation: RRID:Addgene_139344 Copy
Species: Synthetic
Genetic Insert: Scarlet 10AA Neon green
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32191861
Comments: we used #98839 (mScarlet-I-mTurquoise2) as a base for the vector and replaced Turquoise2 with Neon green.
Proper citation: RRID:Addgene_139364 Copy
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