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Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS415; Vector Types:Yeast Expression, Other, Gateway Destination; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14193 Copy
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134979 Copy
Species: Synthetic
Genetic Insert: Hygro-P2A-Clover_PUFa
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR4-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34819316
Proper citation: RRID:Addgene_183348 Copy
Species: Aequorea victoria
Genetic Insert: Venus
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:7470; Vector Backbone:pDEST15; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27455993
Comments: 5' cloning site: AgeI (unknown if destroyed), 3' cloning site: MluI (unknown if destroyed)
Proper citation: RRID:Addgene_128564 Copy
Vector Backbone Description: Vector Backbone:pUC57-R1R4; Vector Types:Other; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23066669
Proper citation: RRID:Addgene_105110 Copy
Vector Backbone Description: Vector Backbone:pXN21-Dest; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18428659
Proper citation: RRID:Addgene_105086 Copy
Vector Backbone Description: Vector Backbone:pFRT-EGFP-DEST; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28297718
Proper citation: RRID:Addgene_106348 Copy
Species: Synthetic
Genetic Insert: beta-lactamase/sfGFP
Vector Backbone Description: Vector Backbone:pGT-Kh2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30643213
Comments: See supplemental tables of the referenced paper for more information on each plasmid.
Proper citation: RRID:Addgene_122587 Copy
Vector Backbone Description: Backbone Size:7763; Vector Backbone:pDEST-CMV-C-EGFP; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_122845 Copy
Vector Backbone Description: Backbone Size:7720; Vector Backbone:pDEST-CMV-N-EGFP; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_122843 Copy
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLV-CMV-y/hNubI-tripleFLAG-linker-Gateway-PuroR; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123221 Copy
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLV-CMV-y/hNubI-tripleFLAG-linker-Gateway-PuroR; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123223 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms.
This vector does not contain a visual marker on the backbone and can be used when the gene of interest has visual phenotype.
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34914 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: New version of Puromycin resistance vector for drug selection in worms.
This vector replaces pBCN22-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells, and the visual marker that expresses well in non-elegans Caenorhabditis species).
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34918 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: New version of Puromycin resistance vector for drug selection in worms.
This vector replaces pBCN21-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells).
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34917 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms.
This vector contains a Pmyo-2::mCherry::myo-2_3'UTR pharyngeal marker on the backbone. Worm strains generated with vector can be crossed with strains generated with other visual markers such as in pBCN41-R4R3 which has a green fluorescent pharyngeal marker.
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34915 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: New version of Neomycin resistance vector for drug selection in worms.
This vector replaces pBCN24-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells, and the visual marker that expresses well in non-elegans Caenorhabditis species).
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34920 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11976; Vector Backbone:pBID-UASC-GRM; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of myc tagRFP (tRFP) fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). myc-tRFP is fused to the Cterminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Proper citation: RRID:Addgene_35203 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11145; Vector Backbone:pBID-UASC-G; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31transgenic Drosophila for GAL4 driven expression. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter(DSCP). Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_35202 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11240; Vector Backbone:pBID-UASC-FG; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of Flag epitope fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). 3 copies of the Flag epitope is fused to the N-terminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_35201 Copy
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