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http://www.addgene.org/44347

Species: Xenopus laevis
Genetic Insert: XOPS-tdTOM
Vector Backbone Description: Backbone Marker:LJ Chang; Backbone Size:8760; Vector Backbone:pTY; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20517486

Proper citation: RRID:Addgene_44347 Copy   

•   Source: Addgene

http://www.addgene.org/47407

Species: Xenopus laevis
Genetic Insert: Intersectin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_47407 Copy   

•   Source: Addgene

http://www.addgene.org/17157

Species: Xenopus laevis
Genetic Insert: siamois promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9308964
Comments: Plasmid History: Constructed by David Kimelman, 6/6/96 XVII p. 98. Notes for Use: The -3.0kb-siamois promoter was directionally cloned into the KpnI and BglII sites of the luciferase reporter vector pGL3B (Promega) upstream of the luciferase ATG. The -0.8kb promoter construct was generated by digesting the -3.0kb/pGL3B construct with KpnI and at an internal EcoRI site to remove 2.2 kb of intervening sequence, filling in with DNA polymerase I large fragment and religating. The Kpn site was lost but the R1 site was regenerated in the ligation. The size of the insert is approximate. Citation: Brannon et al., Gene and Development 11:2359-2370, 1997.

Proper citation: RRID:Addgene_17157 Copy   

•   Source: Addgene

http://www.addgene.org/17146

Species: Xenopus laevis
Genetic Insert: Beta-tubulin promoter
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4300; Vector Backbone:pBLCAT3; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Constructed Rebecca Beach and Paul A. Kreig. 3.8Kb fragment of the Xenopus neural Beta-tubulin promoter inserted (in several cloning steps) into the CAT expression vector pBLCAT3. The 5' end of the neural Beta-tubulin sequence is defined by the natural Xba1 site that occurs in the genomic DNA. The 3' end of the insert is produced by PCR primer located 8bp upstream of hte initiation ATP and 120bp downstream of the transcription start site. The neural Beta-tubulin sequences may be excised as a Hind3 fragment.

Proper citation: RRID:Addgene_17146 Copy   

•   Source: Addgene

http://www.addgene.org/17147

Species: Xenopus laevis
Genetic Insert: NCAM promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin
Comments: Plasmid History: Constructed Paul A. Kreig, July. Note for Use: This construction contains a 1.3 Kb Dra I fragment of the NCAM promoter and should be okay for general neural expression. The fragment is cloned into the Sma I site of Bluescript KS+. The NCAM promoter does not contain a TATA box and transcription starts at numerous sites over about 350 bases. All of the potential start sites are contained in this 1.3 Kb fragment, which ends in the 5' UTR of the NCAM gene. The Dra I fragment starts at the very beginning of the GenBank sequence (starting with aaa) and ends at base 1358 of the GenBank sequence.

Proper citation: RRID:Addgene_17147 Copy   

•   Source: Addgene

http://www.addgene.org/17148

Species: Xenopus laevis
Genetic Insert: cardiac actin promoter
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCSKA; Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin
Comments: Plasmid History: Constructed by Enrique Amaya. Note for Use: Constructed by cloning the 3270bp KpnI-SalI fragment from DNA#254 (from Tim Mohun) into KpnI-SalI digested pCSKA.

Proper citation: RRID:Addgene_17148 Copy   

•   Source: Addgene

http://www.addgene.org/17149

Species: Xenopus laevis
Genetic Insert: cardiac actin promoter
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCSKA; Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin
Comments: Plasmid History: Constructed by Enrique Amaya. Note for Use: Generated by inserting the 1Kb HindIII-NotI fragment from pCSGFP2 into HindIII-NotI digested pCarG. This GFP constuct has been modified by Jim Haseloff and Jonathan Pines to be brighter and more soluble that wild-type GFP. Note: Uniques sites are underlined in Author's Map.

Proper citation: RRID:Addgene_17149 Copy   

•   Source: Addgene

http://www.addgene.org/171548

Species: Xenopus laevis
Genetic Insert: Xlrbpa
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751023

Proper citation: RRID:Addgene_171548 Copy   

•   Source: Addgene

http://www.addgene.org/171547

Species: Xenopus laevis
Genetic Insert: Xlrbpa
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751023

Proper citation: RRID:Addgene_171547 Copy   

•   Source: Addgene

http://www.addgene.org/17158

Species: Xenopus laevis
Genetic Insert: goosecoid promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pOLuc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8543150
Comments: Generated by PCR using the 3' primer (nucleotides - 5 to + 12 relative to the transcription start site) and 5' primer to -226. The 3' primer contained a HindIII restriction site at its 5' terminus and the 5' primer contained a BamHI restriction site at its 5' terminus for directional cloning into pOLuc.

Proper citation: RRID:Addgene_17158 Copy   

•   Source: Addgene

http://www.addgene.org/17159

Species: Xenopus laevis
Genetic Insert: goosecoid promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pOLuc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8543150
Comments: The - 1500gsc/Luc reporter was constructed from a blunt-ended EcoRV-PstI 1.5kb Xenopus gsc gene fragment carrying 3 bp of exon 1 and 5'-promoter sequences inserted into the SmaI site of the promoterless luciferase vector pOLuc (de Wet et al. 1987).

Proper citation: RRID:Addgene_17159 Copy   

•   Source: Addgene

http://www.addgene.org/17191

Species: Xenopus laevis
Genetic Insert: XDpr
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8000; Vector Backbone:pGAD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11970895
Comments: Description: Dpr yeast clone in original prey vector. 1.6kb EcoR1 fragment corresponds to C-terminal 1.2 kb of Xenopus Dpr and 3' UTR in pGAD vector. Reference: Cheyette, B. et al., 2002, Developmental Cell 2: 449-461.

Proper citation: RRID:Addgene_17191 Copy   

•   Source: Addgene

http://www.addgene.org/17192

Species: Xenopus laevis
Genetic Insert: XDpr
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11970895
Comments: Description: Coding sequence of original XDpr prey clone (M35) cloned into pGEX vector as a 1.2 kb EcoR1-Xba1 fragment (Xba site is DAM methylated). Reference: Cheyette, B. et al., 2002, Developmental Cell 2: 449-461.

Proper citation: RRID:Addgene_17192 Copy   

•   Source: Addgene

http://www.addgene.org/17202

Species: Xenopus laevis
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:pET-28 c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Description: This is an amino terminal HIS tag, then beta catenin domains R10C, subcloned into BamH1 site. Reference: Made by Ken-Ichi Takemaru. Contains ARM domains 8-10 and C-terminal end of protein (starts with aa519)

Proper citation: RRID:Addgene_17202 Copy   

•   Source: Addgene

http://www.addgene.org/172464

Species: Xenopus laevis
Genetic Insert: wbp2nlT45A
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28663133
Comments: we cloned this plasmid

Proper citation: RRID:Addgene_172464 Copy   

•   Source: Addgene

http://www.addgene.org/172852

Species: Xenopus laevis
Genetic Insert: xFAM3B.L
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30209221

Proper citation: RRID:Addgene_172852 Copy   

•   Source: Addgene

http://www.addgene.org/16725

Species: Xenopus laevis
Genetic Insert: casein kinase 1, epsilon
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Description: From John Graff, in Peters et al 1999. Notes for Use: Select clones with amp. Reference: used in Waxman JS, Hocking AM, Stoick CL, Moon RT. Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Development. 2004 Dec;131(23):5909-21.

Proper citation: RRID:Addgene_16725 Copy   

•   Source: Addgene

http://www.addgene.org/16704

Species: Xenopus laevis
Genetic Insert: XCtBP
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid History: Constructed by Jeff Brown. Notes for Use: The ~2 kb dropout of the Sal I digest of XTcf-3 interacting clone 2-1 (in pGBT9) was ligated into the XhoI site of pCS2P+. For CS2+MT XCtBP, the Sal I dropout was blunted with Klenow and cloned into the StuI site of pCS2+MT.

Proper citation: RRID:Addgene_16704 Copy   

•   Source: Addgene

http://www.addgene.org/16746

Species: Xenopus laevis
Genetic Insert: XPKC-Alpha
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2869; Vector Backbone:pGem2; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1547501
Comments: SmaI linearize, SP6 for sense RNA. Notes for Use: Ref: used in Otte and Moon, Cell 68:1021-1029, 1992.

Proper citation: RRID:Addgene_16746 Copy   

•   Source: Addgene

http://www.addgene.org/16745

Species: Xenopus laevis
Genetic Insert: XPKC-alpha-myc
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395542
Comments: Insert: Xenopus PKC alpha with a c-myc epitope at carboxy terminus. For transcription cut with Not 1 and use SP6. Notes for Use: Ref: Sheldahl et al., Current Biology 9:695-698, 1999.

Proper citation: RRID:Addgene_16745 Copy   

•   Source: Addgene