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Species: Other
Genetic Insert: AsCas12f1
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_171610 Copy
Species: Streptomyces
Genetic Insert: Streptomyces codon optimized the FnCas12a mutant EP16
Vector Backbone Description: Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_177875 Copy
Species: Synthetic
Genetic Insert: XimD and PcPT
Vector Backbone Description: Vector Backbone:pSE152; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_169109 Copy
Species: Synthetic
Genetic Insert: D4-TheoR-cas9
Vector Backbone Description: Backbone Size:10994; Vector Backbone:pCRISPomyces-2; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_172353 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Jay Keasling lab; Backbone Size:4156; Vector Backbone:pAV-gapdh; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.11.16.384693v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172431 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pACEC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_110085 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_110088 Copy
Species: Synthetic
Genetic Insert: sSpCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_61736 Copy
Species: Synthetic
Genetic Insert: sSpCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_61737 Copy
Species: artificially synthesized, codon optimized gene from Strep pyogenes
Genetic Insert: Scocas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR, Bacterial Genome Editing; Bacterial Resistance:Apramycin
References:
Comments: More information is provided in the supplemental files. Please note that the sequence of the backbone (pKC1139) was obtained via Sanger sequencing and several regions may have discrepancies due to high GC content. These discrepancies have no functional consequences.
Proper citation: RRID:Addgene_62552 Copy
Species: E. coli
Genetic Insert: aac
Vector Backbone Description: Backbone Size:2140; Vector Backbone:pSG5; Vector Types:; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_69615 Copy
Species: E. coli
Genetic Insert: aac
Vector Backbone Description: Backbone Size:2140; Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_69994 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pIJ8668; Vector Types:Bacterial Expression, Streptomyces expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_78576 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5045
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
References:
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167365 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5058
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
References:
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167369 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5041
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
References:
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167363 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5037
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
References:
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167362 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5121
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
References:
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167388 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5114
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
References:
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167387 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5103
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
References:
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167385 Copy
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