Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Xenopus laevis
Genetic Insert: VSP1 C301S
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21618529
Proper citation: RRID:Addgene_51882 Copy
Species: Xenopus laevis
Genetic Insert: Engrailed-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Xenopus Probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1679005
Comments: In situ probe cut with Xba1, transcribe with T3.
Proper citation: RRID:Addgene_16950 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-5A
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8275867
Comments: Insert: cDNA from Melton lambda gt10 oocyte library.
Transcription: linearize with Xba1 and transcribe with SP6.
Plasmid History:
Constructed by Randall Moon on date of 9/15/90.
Notes for Use:
EcoR1 insert of lambda G6 of 2/11/90. Contains significant 5' UTR and is less translatable in vitro per ng RNA than Xwnt-5A Hind III/SP64T. However, the type of activity is the same in both constructs.
Citation: Moon et al., Development 119, 97 (1993)
Proper citation: RRID:Addgene_16985 Copy
Species: Xenopus laevis
Genetic Insert: banded hedgehog
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Xenopus Hedgehog. Antisense are generated as listed:
Xenopus banded hedgehog: Linearize clone XIL in Bluescript KS with BamH1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.
Xenopus sonic hedgehog: Linearize clone X32 in Bluescript KS with Bam H1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.
Xenopus cephalic hedgehog:Linearize clone X30 in pT7TS with Sal 1 and transcribe with T7.
Proper citation: RRID:Addgene_16984 Copy
Species: Xenopus laevis
Genetic Insert: Goosecoid
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Insert: coding region of Xenopus goosecoid in SP64T in sense (#9) & antisense (#2) orientation.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by Jan Christian by PCR.
Goosecoid cloned and characterized by lab of E. DeRobertis, UCLA.
Proper citation: RRID:Addgene_16979 Copy
Species: Xenopus laevis
Genetic Insert: Xbra
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:psp73; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Insert: full length Xbra from Jim Smith.
Antisense: linearize with EcoRV, transcribe with T7.
Plasmid History:
Constructed by Jan Christian.
Proper citation: RRID:Addgene_16978 Copy
Species: Xenopus laevis
Genetic Insert: noggin PCR clone
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pCSKA; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Insert: noggin PCR clone (coding region). In reverse orientation compared to CSKA promoter.
Plasmid History:
Constructed by Jan Christian.
Isolated and characterize by W. Smith and R. Harland.
Proper citation: RRID:Addgene_16998 Copy
Species: Xenopus laevis
Genetic Insert: noggin
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Insert: noggin PCR clone (coding region) in SP64T.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by Jan Christian.
Noggin isolated and chacterized by W. Smith and R. Harland.
Proper citation: RRID:Addgene_16997 Copy
Species: Xenopus laevis
Genetic Insert: Xbhh-C
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7671801
Comments: Expression ConstructXenopus Banded Hedgehog.
(a.k.a. Xbhh; hh1)
Insert: PCR of coding region, Genbank accession number U26404.
Antisense RNA: For in situ we use clone XIL in Bluescript KS. Linearize with Bam H1 and transcribe with T3.
Sense RNA: Linearize with Bam H1 and transcribe with T7.
References:
Ekker et al., Development 121, 2337-2347 (1995)
Lai et al., Development 121, 2349-2360 (1995)
Subclone Xbhh-N in pT7Ts.
Encodes amino terminal product with autoproteolytic cleavage. Linearize with BamH1 and transcribe with T7.
Subclone Xbhh-dominant negative-C in pT7TS:
Encodes a leader sequence, a few amino acids amino terminal to the autoproteolytic site, and the carboxy-terminal domain. Linearize with BamH1 and transcribe with T7.
Proper citation: RRID:Addgene_16995 Copy
Species: Xenopus laevis
Genetic Insert: Xbhh
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7671801
Comments: Expression Construct Xenopus Banded Hedgehog (a.k.a. Xbhh; hh1)
Insert: PCR of coding region, Genbank accession number U26404.
Antisense RNA: For in situ we use clone XIL in Bluescript KS. Linearize with Bam H1 and transcribe with T3.
Sense RNA: Linearize with Bam H1 and transcribe with T7.
References:
Ekker et al., Development 121, 2337-2347 (1995)
Lai et al., Development 121, 2349-2360 (1995)
Proper citation: RRID:Addgene_16993 Copy
Species: Xenopus laevis
Genetic Insert: beta-globin 5' UTR
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCD-PS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Description: This is an old but serviceable SV40 expression construct (pCD-PS) modified to give the Xenopus beta-globin untranslated regions and multiple cloning sites from pT7TS vector (Addgene plasmid 17091).
Reference: The vector was made by Julia Yang-Snyder.
Proper citation: RRID:Addgene_17120 Copy
Species: Xenopus laevis
Genetic Insert: Ecd8
Vector Backbone Description: Backbone Marker:R. Freeman/ S. Sokol; Backbone Size:3000; Vector Backbone:pXT7; Vector Types:Xenopus RNA expression ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10485853
Comments: Digest with Pst1, transcribe with T7 pol for sense RNA.
Proper citation: RRID:Addgene_32992 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt2b
Vector Backbone Description: Backbone Marker:R. Freeman/ S. Sokol; Backbone Size:3000; Vector Backbone:pXT7; Vector Types:RNA Synthesis and Injections; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9203142
Comments: Addgene quality control sequencing discovered a P159A point mutation. According to the depositing lab, this mutation is not a concern, as the product is functionally active.
Proper citation: RRID:Addgene_32988 Copy
Species: Xenopus laevis
Genetic Insert: Smurf1
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10458166
Proper citation: RRID:Addgene_117244 Copy
Species: Xenopus laevis
Genetic Insert: Tbx5-EnR
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10079235
Proper citation: RRID:Addgene_117249 Copy
Species: Xenopus laevis
Genetic Insert: BRAT
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9165117
Proper citation: RRID:Addgene_117251 Copy
Species: Xenopus laevis
Genetic Insert: Tbx5-EnR-GR
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10079235
Proper citation: RRID:Addgene_117250 Copy
Species: Xenopus laevis
Genetic Insert: Sizzled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9428410
Comments: This encodes a secreted
antagonist of Xwnt-8.
The entire open reading frame of sizzled was amplified by low-cycle number PCR using Pfu polymerase and cloned as a
BamHI-XhoI fragment into pCS2+ .
Proper citation: RRID:Addgene_16688 Copy
Species: Xenopus laevis
Genetic Insert: casein kinase 1, epsilon
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Description: From John Graff, in Peters et al 1999.
Notes for Use: Select clones with amp.
Reference: used in Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_16725 Copy
Species: Xenopus laevis
Genetic Insert: XCtBP
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid History:
Constructed by Jeff Brown.
Notes for Use:
The ~2 kb dropout of the Sal I digest of XTcf-3 interacting clone 2-1 (in pGBT9) was ligated into the XhoI site of pCS2P+. For CS2+MT XCtBP, the Sal I dropout was blunted with Klenow and cloned into the StuI site of pCS2+MT.
Proper citation: RRID:Addgene_16704 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the PRECISE-TBI Resources search. From here you can search through a compilation of resources used by PRECISE-TBI and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that PRECISE-TBI has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on PRECISE-TBI then you can log in from here to get additional features in PRECISE-TBI such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into PRECISE-TBI you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within PRECISE-TBI that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
