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Species: Danio rerio
Genetic Insert: Fgf2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16862139
Proper citation: RRID:Addgene_12309 Copy
Species: Danio rerio
Genetic Insert: rag1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS(-); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9089097
Proper citation: RRID:Addgene_11299 Copy
Species: Danio rerio
Genetic Insert: IgM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3948; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15685175
Comments: Clone A1 (exon 1-3) was amplified using primers zfCH1 (5'-TCAACCATCTGCGCCCCAGT) and cm3A (5'-TCCTGAGGCTTTGCACTT).
Proper citation: RRID:Addgene_11301 Copy
Species: Danio rerio
Genetic Insert: rag1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS(-); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9089097
Comments: This is a rag1 PCR product. To make probes: antisense HindIII - T7, sense XbaI - T3.
Proper citation: RRID:Addgene_11300 Copy
Species: Danio rerio
Genetic Insert: ZFERV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII-TOPO; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14694121
Comments: ZFERV PCR insert (the single 3' A overhangs on both ends are not included): 5'-TCTAAAGGAAAATGAACTTAACAGTTGCGAG (31 nt total upstream of the ZFERV sequence), CGAGgacaac agtggaatgg aatctttgtt aaatttagca
aagtacagta aactgaacag agtattgcga ata -3'
(73 nt total downstream of the ZFERV sequence).
A map of pCRII-TOPO is available at Addgene's vector DB.
Proper citation: RRID:Addgene_11192 Copy
Species: Danio rerio
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117766 Copy
Species: Danio rerio
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117768 Copy
Species: Danio rerio
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2329; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Control integrates at the gene cx43.4 using the guide RNA Target "5'-GGGCCATATCTTGCCCACGA-3'" for wide-spread RFP expression in zebrafish embryos from 12hrs post injection through 5 days development
Proper citation: RRID:Addgene_117801 Copy
Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117792 Copy
Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-eGFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117791 Copy
Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117793 Copy
Species: Danio rerio
Genetic Insert: mRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117784 Copy
Species: Danio rerio
Genetic Insert: mRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117786 Copy
Species: Danio rerio
Genetic Insert: gal4/VP16
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117781 Copy
Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-TagBFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117789 Copy
Species: Danio rerio
Genetic Insert: mRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-eGFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117788 Copy
Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117790 Copy
Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-eGFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117773 Copy
Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP, Oline tools and cloning help avalible at
Proper citation: RRID:Addgene_117775 Copy
Species: Danio rerio
Genetic Insert: 3x3 Stop sequence
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117772 Copy
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